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2451 Lambda-PRL2 Arabidopsis thaliana cDNA clone 81A8T7, mRNA sequence

GenBank: T20443.1

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LOCUS       T20443                   389 bp    mRNA    linear   EST 28-JAN-2011
DEFINITION  2451 Lambda-PRL2 Arabidopsis thaliana cDNA clone 81A8T7, mRNA
            sequence.
ACCESSION   T20443
VERSION     T20443.1
DBLINK      BioSample: SAMN00154689
KEYWORDS    EST.
SOURCE      Arabidopsis thaliana (thale cress)
  ORGANISM  Arabidopsis thaliana
            Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
            Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae;
            Pentapetalae; rosids; malvids; Brassicales; Brassicaceae;
            Camelineae; Arabidopsis.
REFERENCE   1  (bases 1 to 389)
  AUTHORS   Newman,T., deBruijn,F.J., Green,P., Keegstra,K., Kende,H.,
            McIntosh,L., Ohlrogge,J., Raikhel,N., Somerville,S., Thomashow,M.,
            Retzel,E. and Somerville,C.
  TITLE     Genes galore: a summary of methods for accessing results from
            large-scale partial sequencing of anonymous Arabidopsis cDNA clones
  JOURNAL   Plant Physiol. 106, 1241-1255 (1994)
   PUBMED   7846151
COMMENT     Contact: Thomas Newman
            MSU-DOE Plant Research Laboratory
            Michigan State University
            MSU-DOE-PRL, Michigan State University,Plant Biology Bldg.,E.
            Lansing,Mi
            Tel: 517-353-0854
            Fax: 517-353-9168
            Email: 22313tcn@ibm.cl.msu.edu
            Seq primer: T7.
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Arabidopsis thaliana"
                     /mol_type="mRNA"
                     /db_xref="taxon:3702"
                     /clone="81A8T7"
                     /clone_lib="SAMN00154689 Lambda-PRL2"
                     /ecotype="Columbia"
                     /note="Vector: lambda Zip-Lox; Site_1: Sal; Site_2: Not;
                     Lambda PRL2 is a cDNA library derived from equal
                     quantities of 4 pools of mRNA. The mRNA sources were 1) 7
                     day germinated etiolated seedlings; 2) tissue culture
                     grown roots; 3) staged plants half with 24 hour light
                     cycle, half on 16 hr light, 8 hour dark- rosettes; 4) same
                     plants as 3 but aerial tissue (stems, flowers and
                     siliques. The vector is BRL's lambda Zip-Lox. The cDNA
                     inserts were directionally cloned with Sal-Not arms using
                     oligo dT primed cDNA."
ORIGIN      
        1 tgtttcccaa aatcccaaat atgtgactcc aancgacaag ttcttctata aaaagtaact
       61 cctctatact gtgaacttct agaccttggt cctctcngac tctttttaac ctgagccgga
      121 accggagccg gagtcggaac cggagttacc aatttcgttt ctccgtcacc aatanggtca
      181 aaagaaagat ctatccagtt tctagagctt gatgatcctt caacatctcg tagatgtcca
      241 cagtctccac tcaccggaaa aaactcttta gtnacggaag ctgaagcggc ggggctttcg
      301 tctccgccgc tactacttcc gacttttaaa atatcgaaac tgagagtgaa agctcgagta
      361 gagcaagagt cttcgtctcc atcggcatt
//
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