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16551 Lambda-PRL2 Arabidopsis thaliana cDNA clone 191C12T7, mRNA sequence

GenBank: R90196.1

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LOCUS       R90196                   479 bp    mRNA    linear   EST 28-JAN-2011
DEFINITION  16551 Lambda-PRL2 Arabidopsis thaliana cDNA clone 191C12T7, mRNA
            sequence.
ACCESSION   R90196
VERSION     R90196.1
DBLINK      BioSample: SAMN00154689
KEYWORDS    EST.
SOURCE      Arabidopsis thaliana (thale cress)
  ORGANISM  Arabidopsis thaliana
            Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
            Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae;
            Pentapetalae; rosids; malvids; Brassicales; Brassicaceae;
            Camelineae; Arabidopsis.
REFERENCE   1  (bases 1 to 479)
  AUTHORS   Newman,T., deBruijn,F.J., Green,P., Keegstra,K., Kende,H.,
            McIntosh,L., Ohlrogge,J., Raikhel,N., Somerville,S., Thomashow,M.,
            Retzel,E. and Somerville,C.
  TITLE     Genes galore: a summary of methods for accessing results from
            large-scale partial sequencing of anonymous Arabidopsis cDNA clones
  JOURNAL   Plant Physiol. 106, 1241-1255 (1994)
   PUBMED   7846151
COMMENT     Contact: Thomas Newman
            MSU-DOE Plant Research Laboratory
            Michigan State University
            MSU-DOE-PRL, Michigan State University,Plant Biology Bldg.,E.
            Lansing,Mi
            Tel: 517-353-0854
            Fax: 517-353-9168
            Email: 22313tcn@ibm.cl.msu.edu
            Seq primer: T7 dye primer.
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Arabidopsis thaliana"
                     /mol_type="mRNA"
                     /db_xref="taxon:3702"
                     /clone="191C12T7"
                     /clone_lib="SAMN00154689 Lambda-PRL2"
                     /ecotype="Columbia"
                     /note="Vector: lambda Zip-Lox; Site_1: Sal; Site_2: Not;
                     Lambda PRL2 is a cDNA library derived from equal
                     quantities of 4 pools of mRNA. The mRNA sources were 1) 7
                     day germinated etiolated seedlings; 2) tissue culture
                     grown roots; 3) staged plants half with 24 hour light
                     cycle, half on 16 hr light, 8 hour dark- rosettes; 4) same
                     plants as 3 but aerial tissue (stems, flowers and
                     siliques. The vector is BRL's lambda Zip-Lox. The cDNA
                     inserts were directionally cloned with Sal-Not arms using
                     oligo dT primed cDNA."
ORIGIN      
        1 gacagacaaa acactcacca ccctcaccaa acagtctctn ttcaactcta aaatcaggtg
       61 gcaaaacaaa atcaatggct ctagtggtgt caggtggcaa agcagccatg atgccgcttc
      121 atgccaattn ttttcctcta agtatcaaca ccaaatcaag agtactaagt gcatcagcat
      181 ttccactatt ctggtccacn cctcacctcc cttccagatc cctctccatc cgcctctggc
      241 cgaatgtttc cnggtctctc actgtggtat cttctgtttt gtcagaggnc agagctacta
      301 acgttagtgg ggtctggggc agatgcattc aagctgnctt acttggnggg aatagctggc
      361 tatgggaaca gctgggctna aaacctagtt gatccggttc ttntgggaat ttgggctttg
      421 ggatnccntg gtttttnatg ntnccagngg tttttnaggg cttcaaggtt nntgncccc
//
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