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yj54e12.s1 Soares breast 2NbHBst Homo sapiens cDNA clone IMAGE:152590 3', mRNA sequence

GenBank: R50116.1

FASTA Graphics 

LOCUS       R50116                   460 bp    mRNA    linear   EST 18-MAY-1995
DEFINITION  yj54e12.s1 Soares breast 2NbHBst Homo sapiens cDNA clone
            IMAGE:152590 3', mRNA sequence.
ACCESSION   R50116
VERSION     R50116.1
DBLINK      BioSample: SAMN00154892
KEYWORDS    EST.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Hillier,L., Clark,N., Dubuque,T., Elliston,K., Hawkins,M.,
            Holman,M., Hultman,M., Kucaba,T., Le,M., Lennon,G., Marra,M.,
            Parsons,J., Rifkin,L., Rohlfing,T., Soares,M., Tan,F.,
            Trevaskis,E., Waterston,R., Williamson,A., Wohldmann,P. and
            Wilson,R.
  TITLE     The WashU-Merck EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Wilson RK
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: est@watson.wustl.edu
            Insert Size: 941
            High quality sequence stops: 286 Source: IMAGE Consortium, LLNL
            This clone is available royalty-free through LLNL ; contact the
            IMAGE Consortium (info@image.llnl.gov) for further information.
            Insert Length: 941   Std Error: 0.00
            Seq primer: Promega -21m13
            High quality sequence stop: 286.
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="GDB:564815"
                     /db_xref="taxon:9606"
                     /clone="IMAGE:152590"
                     /sex="female"
                     /clone_lib="SAMN00154892 Soares breast 2NbHBst"
                     /dev_stage="adult"
                     /lab_host="DH10B (ampicillin resistant)"
                     /note="Organ: breast; Vector: pT7T3D (Pharmacia) with a
                     modified polylinker; Site_1: Not I; Site_2: Eco RI; 1st
                     strand cDNA was primed with a Not I - oligo(dT) primer [5'
                     TGTTACCAATCTGAAGTGGGAGCGGCCGCCCTTTTTTTTTTTTTTTTTTT 3'],
                     double-stranded cDNA was ligated to Eco RI adaptors
                     (Pharmacia), digested with Not I and cloned into the Not I
                     and Eco RI sites of a modified pT7T3 vector (Pharmacia).
                     Library went through one round of normalization to a Cot =
                     230. Library constructed by Bento Soares and M.Fatima
                     Bonaldo."
ORIGIN      
        1 ccagtgttga caaatggttg tttattggtc tgttacacag aagagtatag tttctgaggc
       61 aaaaatcata cacactctgc tcctcacaaa accacggagt cactaaggca ggcacacaat
      121 ggccacacgt gcttggtgga ggccaaggac tcacggacaa caaggatgga caatggggtg
      181 gtctggcccc cagacaggct gtgcagtgcc cgggggaggg ctgggctggg caggctntgc
      241 tgggtgcaga agggctgcgg ggccctttgt catgtttctc aaatgcagaa agtntgccag
      301 antttgggtg gctgctggac tagggcccca gccccgggca gggccttcca cgtttccagg
      361 ggcaaggnct tagnctgcat ntttttgggg gtagaaggng ggaagtnggg gatnggccca
      421 gcaagcagca tgcctagggc tggggggnag gagntttgcc
//
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