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yx49c10.s1 Soares melanocyte 2NbHM Homo sapiens cDNA clone IMAGE:265074 3', mRNA sequence

GenBank: N21329.1

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LOCUS       N21329                   460 bp    mRNA    linear   EST 19-DEC-1995
DEFINITION  yx49c10.s1 Soares melanocyte 2NbHM Homo sapiens cDNA clone
            IMAGE:265074 3', mRNA sequence.
ACCESSION   N21329
VERSION     N21329.1
DBLINK      BioSample: SAMN00154979
KEYWORDS    EST.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Hillier,L., Clark,N., Dubuque,T., Elliston,K., Hawkins,M.,
            Holman,M., Hultman,M., Kucaba,T., Le,M., Lennon,G., Marra,M.,
            Parsons,J., Rifkin,L., Rohlfing,T., Soares,M., Tan,F.,
            Trevaskis,E., Waterston,R., Williamson,A., Wohldmann,P. and
            Wilson,R.
  TITLE     The WashU-Merck EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Wilson RK
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: est@watson.wustl.edu
            Source: IMAGE Consortium, LLNL
            This clone is available royalty-free through LLNL ; contact the
            IMAGE Consortium (info@image.llnl.gov) for further information.
            Insert Length: 999   Std Error: 0.00
            Seq primer: m13 -40 forward.
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="GDB:3874716"
                     /db_xref="taxon:9606"
                     /clone="IMAGE:265074"
                     /sex="male"
                     /tissue_type="melanocyte"
                     /clone_lib="SAMN00154979 Soares melanocyte 2NbHM"
                     /lab_host="DH10B (ampicillin resistant)"
                     /note="Vector: pT7T3D (Pharmacia) with a modified
                     polylinker; Site_1: Not I; Site_2: Eco RI; 1st strand cDNA
                     was primed with a Not I - oligo(dT) primer [5'
                     TGTTACCAATCTGAAGTGGGAGCGGCCGCAGTTTTTTTTTTTTTTTTTTT 3'],
                     double-stranded cDNA was size selected, ligated to Eco RI
                     adapters (Pharmacia), digested with Not I and cloned into
                     the Not I and Eco RI sites of a modified pT7T3 vector
                     (Pharmacia). Library constructed by Bento Soares and
                     M.Fatima Bonaldo. RNA from normal foreskin melanocytes
                     (FS374) was kindly provided by Dr. Anthony P. Albino."
ORIGIN      
        1 cagtgtttaa gtattttatt aaatcagaca aggaatctcc acactgttgc acataacagc
       61 ttttatacaa tgataaggac atatcatttg tttacaaaga aagtctaaaa tttcaagaac
      121 attcaaagag ctaacacagt aaaggtcatg caagttctag aatagtgaat catgacagaa
      181 ctcattcatt ttatccttta cctccaaaag gcccatctcc ttaacgagaa gacatctcaa
      241 gaccaggagc ttgtcactag tctgatattt cattcaggaa tattgagcct gttagcacgt
      301 actggcttga taggaagtaa ctcaacccta actgtagaaa agggttttct gaagagactc
      361 actgctgcaa aatgcatgcc ctgtattcat attgtgttat acgatgaaca tgccacatgc
      421 tttcatttaa gtacgtgtgc gtaacacccg aaccaggaat
//
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