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yx42c09.s1 Soares melanocyte 2NbHM Homo sapiens cDNA clone IMAGE:264400 3', mRNA sequence

GenBank: N21217.1

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LOCUS       N21217                   463 bp    mRNA    linear   EST 19-DEC-1995
DEFINITION  yx42c09.s1 Soares melanocyte 2NbHM Homo sapiens cDNA clone
            IMAGE:264400 3', mRNA sequence.
ACCESSION   N21217
VERSION     N21217.1
DBLINK      BioSample: SAMN00154979
KEYWORDS    EST.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 463)
  AUTHORS   Hillier,L., Clark,N., Dubuque,T., Elliston,K., Hawkins,M.,
            Holman,M., Hultman,M., Kucaba,T., Le,M., Lennon,G., Marra,M.,
            Parsons,J., Rifkin,L., Rohlfing,T., Soares,M., Tan,F.,
            Trevaskis,E., Waterston,R., Williamson,A., Wohldmann,P. and
            Wilson,R.
  TITLE     The WashU-Merck EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Wilson RK
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: est@watson.wustl.edu
            High quality sequence stops: 258
            Source: IMAGE Consortium, LLNL
            This clone is available royalty-free through LLNL ; contact the
            IMAGE Consortium (info@image.llnl.gov) for further information.
            Insert Length: 1404   Std Error: 0.00
            Seq primer: m13 -40 forward
            High quality sequence stop: 258.
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="GDB:3874042"
                     /db_xref="taxon:9606"
                     /clone="IMAGE:264400"
                     /sex="male"
                     /tissue_type="melanocyte"
                     /clone_lib="SAMN00154979 Soares melanocyte 2NbHM"
                     /lab_host="DH10B (ampicillin resistant)"
                     /note="Vector: pT7T3D (Pharmacia) with a modified
                     polylinker; Site_1: Not I; Site_2: Eco RI; 1st strand cDNA
                     was primed with a Not I - oligo(dT) primer [5'
                     TGTTACCAATCTGAAGTGGGAGCGGCCGCAGTTTTTTTTTTTTTTTTTTT 3'],
                     double-stranded cDNA was size selected, ligated to Eco RI
                     adapters (Pharmacia), digested with Not I and cloned into
                     the Not I and Eco RI sites of a modified pT7T3 vector
                     (Pharmacia). Library constructed by Bento Soares and
                     M.Fatima Bonaldo. RNA from normal foreskin melanocytes
                     (FS374) was kindly provided by Dr. Anthony P. Albino."
ORIGIN      
        1 nttttttctg gatctgaaaa taatcaaaaa ttttatttta attcacattt atttaaatat
       61 aacatttttt atagacacat gaaggtactt atgaacattt ataacatggt aggcacaaat
      121 atagatgaaa tgtttaggta ttttcgacaa aatttcacac aaaattagac aaactgacta
      181 tgtgcttgca tttaatttca agggtacatt aacatagata tttgaaaagg atgttctttt
      241 acaaaactac tacaaaatgt tcacatttgc atcagtaata agtcatattt cctagactga
      301 tgctttgtaa gaactatgta aaganctaat ttaatcatta acaccatatg ggtaacntta
      361 tatggtaaat gaagcntatt tttgnccaat agaaatgntc cccnaacctt taaaataacn
      421 taggcntttg gantaaaacc ntggccncat ttattaaacc aac
//
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