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yx08d10.s1 Soares melanocyte 2NbHM Homo sapiens cDNA clone IMAGE:261139 3', mRNA sequence

GenBank: H98193.1

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LOCUS       H98193                   514 bp    mRNA    linear   EST 12-DEC-1995
DEFINITION  yx08d10.s1 Soares melanocyte 2NbHM Homo sapiens cDNA clone
            IMAGE:261139 3', mRNA sequence.
ACCESSION   H98193
VERSION     H98193.1
DBLINK      BioSample: SAMN00154979
KEYWORDS    EST.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Hillier,L., Clark,N., Dubuque,T., Elliston,K., Hawkins,M.,
            Holman,M., Hultman,M., Kucaba,T., Le,M., Lennon,G., Marra,M.,
            Parsons,J., Rifkin,L., Rohlfing,T., Soares,M., Tan,F.,
            Trevaskis,E., Waterston,R., Williamson,A., Wohldmann,P. and
            Wilson,R.
  TITLE     The WashU-Merck EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Wilson RK
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: est@watson.wustl.edu
            High quality sequence stops: 311
            Source: IMAGE Consortium, LLNL
            This clone is available royalty-free through LLNL ; contact the
            IMAGE Consortium (info@image.llnl.gov) for further information.
            Insert Length: 1611   Std Error: 0.00
            Seq primer: m13 -40 forward
            High quality sequence stop: 311.
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="GDB:3870781"
                     /db_xref="taxon:9606"
                     /clone="IMAGE:261139"
                     /sex="male"
                     /tissue_type="melanocyte"
                     /clone_lib="SAMN00154979 Soares melanocyte 2NbHM"
                     /lab_host="DH10B (ampicillin resistant)"
                     /note="Vector: pT7T3D (Pharmacia) with a modified
                     polylinker; Site_1: Not I; Site_2: Eco RI; 1st strand cDNA
                     was primed with a Not I - oligo(dT) primer [5'
                     TGTTACCAATCTGAAGTGGGAGCGGCCGCAGTTTTTTTTTTTTTTTTTTT 3'],
                     double-stranded cDNA was size selected, ligated to Eco RI
                     adapters (Pharmacia), digested with Not I and cloned into
                     the Not I and Eco RI sites of a modified pT7T3 vector
                     (Pharmacia). Library constructed by Bento Soares and
                     M.Fatima Bonaldo. RNA from normal foreskin melanocytes
                     (FS374) was kindly provided by Dr. Anthony P. Albino."
ORIGIN      
        1 agtgtaaaca ttcacatatt taatagtacc tttaaaataa gcattactac atttaaaatg
       61 gttccaaaat gaatctataa atggtaatat aaattaaaaa atacgaactt aaagtgaata
      121 aatttttaac cttagctatg gtataaataa tggtaaatgt atagtgtacc tttgagtcat
      181 taaaatgtct taaaagataa cagcttgtta ccagaacatt agaaaccata gccatgattc
      241 tcaagcgtta acaatctaca tttgatattt tcttggccac tgcattcttc aatgaataat
      301 aatttccaga attccattcc atgttgtttt ccaatagatc ttttcacact cgatgttgaa
      361 tacatggctc tttgcagatg aataaaacaa gtgcattcag tttagagatc cattttgcga
      421 aggctcattc gactgaagga atctctgtgg gcaggtgaca ggcaatactg ccntgtataa
      481 ctgagggatc tttcngttga tcagtggctg tagg
//
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