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AGENCOURT_28538078 NIH_MGC_246 Rattus norvegicus cDNA clone IMAGE:7372364 5', mRNA sequence

GenBank: CO573921.1

FASTA Graphics 

LOCUS       CO573921                 750 bp    mRNA    linear   EST 11-JAN-2011
DEFINITION  AGENCOURT_28538078 NIH_MGC_246 Rattus norvegicus cDNA clone
            IMAGE:7372364 5', mRNA sequence.
ACCESSION   CO573921
VERSION     CO573921.1
DBLINK      BioSample: SAMN00175240
KEYWORDS    EST.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 750)
  CONSRTM   National Institutes of Health, Mammalian Gene Collection (MGC)
  TITLE     NIH-MGC EST Sequencing Project
  JOURNAL   Unpublished
  REMARK    http://mgc.nci.nih.gov/
COMMENT     Contact: Daniela S. Gerhard, Ph.D.
            Office of Cancer Genomics
            National Cancer Institute / NIH
            Bldg. 31 Rm10A07 Bethesda, MD 20892
            Email: cgapbs-r@mail.nih.gov
            Tissue Procurement: Drs. Josef Lazar & Howard Jacob, Medical
            College of Wisconsin
             cDNA Library Preparation: Open Biosystems
             cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
             DNA Sequencing by: Agencourt Bioscience Corporation
             Clone distribution: MGC clone distribution information can be
            found through the I.M.A.G.E. Consortium/LLNL at:
            http://image.llnl.gov
            
            Plate: LLAM15517  row: o  column: 18
            High quality sequence stop: 663.
FEATURES             Location/Qualifiers
     source          1..750
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /clone="IMAGE:7372364"
                     /clone_lib="SAMN00175240 NIH_MGC_246"
                     /lab_host="DH10B TonA"
                     /note="Organ: liver; Vector: pExpress-1; Site_1: EcoRV;
                     Site_2: NotI; RNA obtained from testis tissue of 8 wk old
                     animal. Tissues were snap-frozen and kept at -80C before
                     RNA extraction and purification (Tri-reagent method). cDNA
                     was primed using oligo-dT primer:
                     5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)25-3' and cloned into
                     the EcoRV/NotI sites of pExpress-1. Size-selection >1.25kb
                     resulted in an average insert size of 1.7 kb. This a
                     primary library (normalized library is NIH_MGC_247) and
                     was constructed by Open Biosystems. Note: this is a
                     NIH_MGC library"
ORIGIN      
        1 ctgaggcagt cagccgactc acttctgttc aaaatggccc catttttctg attctgggag
       61 acctgctgga gaccaggccc aaggttgctg agtcattcct cacttcttcc caacagcctc
      121 agtccccatt tatcaggtct ccatgctctg tgatctatgc tattggacta tgtataaggt
      181 ctgaattcta tgtctacagg tttgcctagt atgtatggtt catgtaaaca ggatcatata
      241 gtgtatgaac tttgttgtct tgtgtctact tcacacatta gttttcgata tcctacaggc
      301 catagtcaca caccttcatg agcctttctc atgatctttc ttagtatcct cttcattcac
      361 cttctgtcta ggcagaagcc tcctcagcct tgagctgcat gtttcttaac atttacctta
      421 ccttctgtta tgattctttt tagatctccc aagaatcaca tcaatatctg cattagtaat
      481 ggttcttctc taaagtaata ggactgacaa aatcaatcaa gcatccaggc aggcatggta
      541 cagaaggagc taagacttcc acatctttat ctgaaggctg ctaagataat actggcttca
      601 ggcagctaga aagagggtct tatagctcat acccacagtg acacacctac tccatcaggg
      661 nccacacttc taatagtgcc actccctggg tcaaaactca cttctctgca tgacacttca
      721 gtctcagctt tcattgcact gagctgcact
//
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