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BX111003 Soares_parathyroid_tumor_NbHPA Homo sapiens cDNA clone IMAGp998E01730; IMAGE:322536 5', mRNA sequence

GenBank: BX111003.1

FASTA Graphics 

LOCUS       BX111003                 729 bp    mRNA    linear   EST 07-FEB-2003
DEFINITION  BX111003 Soares_parathyroid_tumor_NbHPA Homo sapiens cDNA clone
            IMAGp998E01730; IMAGE:322536 5', mRNA sequence.
ACCESSION   BX111003
VERSION     BX111003.1
DBLINK      BioSample: SAMN00155028
KEYWORDS    EST.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 729)
  AUTHORS   Ebert,L., Heil,O., Hennig,S., Neubert,P., Partsch,E., Peters,M.,
            Radelof,U., Schneider,D. and Korn,B.
  TITLE     Human UnigeneSet - RZPD3
  JOURNAL   Unpublished (2003)
COMMENT     Contact: Ina Rolfs
            RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH
            Im Neuenheimer Feld 580, D-69120 Heidelberg, Germany
            RZPD; IMAGp998E01730.
            RZPDLIB; I.M.A.G.E. cDNA Clone Collection;
            Human UnigeneSet - RZPD3 (RZPDLIB No.972)
            http://www.rzpd.de/CloneCards/cgi-
            bin/showLib.pl.cgi/response?libNo=972 Contact: Ina Rolfs
            RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH
            Heubnerweg 6, D-14059 Berlin, Germany
            Tel: +49 30 32639 101
            Fax: +49 30 32639 111
            www.rzpd.de
            This clone is available royalty-free from RZPD;
            contact RZPD (clone@rzpd.de) for further information. Seq primer:
            M13r, Primer sequence: TTTCACACAGGAAACAGCTATGAC.
FEATURES             Location/Qualifiers
     source          1..729
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /clone="IMAGp998E01730; IMAGE:322536"
                     /tissue_type="parathyroid tumor"
                     /clone_lib="SAMN00155028 Soares_parathyroid_tumor_NbHPA"
                     /dev_stage="adult"
                     /lab_host="DH10B (ampicillin resistant)"
                     /note="Organ: parathyroid gland; Vector: pT7T3D
                     (Pharmacia) with a modified polylinker; Site_1: Not I;
                     Site_2: Eco RI; 1st strand cDNA was primed with a Not I -
                     oligo(dT) primer
                     [5'-
                     TGTTACCAATCTGAAGTGGGAGCGGCCGCACCAATTTTTTTTTTTTTTTTTTTTTTTT
                     T-3'], double-stranded cDNA was size selected, ligated to
                     Eco RI adapters (Pharmacia), digested with Not I and
                     cloned into the Not I and Eco RI sites of a modified pT7T3
                     vector (Pharmacia). Library went through one round of
                     normalization to a Cot = 5. Library constructed by Bento
                     Soares and M.Fatima Bonaldo. RNA from sporadic parathyroid
                     adenomas was kindly provided by Dr. Stephen Marx, National
                     Institute of Diabetes and Digestive and Kidney Diseases,
                     NIH."
ORIGIN      
        1 taccaatctg aagtgggcct ccagacaccc tgagctgcat ggagaacagc aagggccttt
       61 tgctcatctg cagtgtacag cactgcctct acttcatctt gatgctgctc ctggtcagtc
      121 ttctcggcac gctggggacc tccttactca cagacccaat tcctgttaaa caagttaagt
      181 gctgccaggt agggtgctct ggggtggacc ctgcagttct agggagtcac tgaagaatag
      241 ggaaattcct gcattgtctt tggaaagggg agggtttggg ggagcattgg ggtgggcaga
      301 aacaggcagg tacattttaa agggctcagc agagtgaaac aaagctgaac tcaaccagat
      361 gtcctgcagc gatttgccag gtattccttt agatcgagaa gtttggcttt taaaatgcaa
      421 attttccttg gaagttttaa atccaccaac cttagtatga attaatgcaa tcagggcact
      481 cccagctgtt acccacccag ggggaatgaa gccaatggac attcagcccc cactgtagtg
      541 cagtaggtga gagcatggca ggctctggag cagactgcca ggatgccgat cctagccttg
      601 cccttactag tgtgtgtgat cccggatgaa tatttaactt ttctgtggct cagttaaatg
      661 gatgtaataa tatctacctc aaagatcagt tatgaggatt aaatgagtta atcaatgtaa
      721 agcacttan
//
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