U.S. flag

An official website of the United States government

CDK7_2764 Rhesus macaque genomic DNA Macaca mulatta STS genomic clone MMA2764, sequence tagged site

GenBank: BV448093.1

FASTA Graphics 

LOCUS       BV448093                 375 bp    DNA     linear   STS 05-MAR-2005
DEFINITION  CDK7_2764 Rhesus macaque genomic DNA Macaca mulatta STS genomic
            clone MMA2764, sequence tagged site.
ACCESSION   BV448093
VERSION     BV448093.1
KEYWORDS    STS.
SOURCE      Macaca mulatta (Rhesus monkey)
  ORGANISM  Macaca mulatta
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Cercopithecidae; Cercopithecinae; Macaca.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Spindel,E.R., Pauley,M., Jia,Y., Thompson,S., Lankhorst,M.,
            Gravett,C., Lupo,S.L., Tchourbanov,A., Ali,H., Ojeda,S.R. and
            Norgren,R.B.
  TITLE     Targeted amplification of the 3' end of rhesus macaque orthologs of
            human genes
  JOURNAL   Unpublished
COMMENT     
            Contact: Spindel ER
            Division of Neuroscience
            Oregon National Primate Research Center
            505 NW 185th Avenue, Beaverton, OR 97006, USA
            Tel: 403-690-5388
            Fax: 503-690-5384
            Email: spindele@ohsu.edu
            Primer A: atgtgaactttgttaaatgtg
            Primer B: actacccatgctactacagat
            STS size: 375
            PCR Profile:
                  Hot Start:       95 degrees C for 2.00 min
                  Denaturation:    95 degrees C for 0.50 min
                  Annealing:       51 degrees C for 0.50 min
                  Polymerization:  72 degrees C for 1.00 min
                  PCR Cycles:      35
                  Extension        72 degrees C for 7.0 min
                  Thermal Cycler:  MJ Instruments PTC100
            Protocol:
                       Template:        200 ng
                       Primer:          each 1uM
                       dNTP's:          each 200 uM
                       Taq Polymerase:  0.05 units/ul (Fast Start High
            Fidelity, Roche)
                       Total Vol:       50 ul
            
            Buffer:
                      MgCl2:           1.8 mM
                      Fast Start polymerase reaction buffer (Roche)
            
            Bases 36-347 are 93% homologous (Blast) to bases 1099-1413 of
            NM_001799.2.   Primers were chosen to amplify genomic DNA in the 3'
            region of CDK7.  As human sequence was used to design the primers,
            the primer sequences are not included in the rhesus sequence
            provided below.   To obtain additional information regarding
            primers or clones contact:  Dr. Robert Norgren; Dept of Genetics,
            Cell Biology & Anatomy; University of Nebraska Medical Center;
            986395 Nebraska Medical Center; Omaha, NE  68198.  Email:
            rnorgren@unmc.edu
            A database containing sequences associated with this project can be
            found at:  http://rhesusgenechip.unomaha.edu/index.html.
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Macaca mulatta"
                     /mol_type="genomic DNA"
                     /strain="Indian orgin"
                     /db_xref="taxon:9544"
                     /clone="MMA2764"
                     /clone_lib="Rhesus macaque genomic DNA"
                     /dev_stage="Adult"
                     /note="Organ: Liver; Vector: pGEM-T Easy; V-type: Plasmid;
                     STS was amplified from rhesus genomic DNA with the human
                     forward and reverse primers listed above and subcloned
                     into pGEM-T Easy"
     gene            1..375
                     /gene="CDK7"
                     /note="cyclin-dependent kinase 7"
     STS             <1..>375
                     /gene="CDK7"
ORIGIN      
        1 aacaacttct tttttttttt ttctttttgc tttctaggag gattgcccaa gaaactaatt
       61 ttttaaagag aacactggac aacattttcc tactgaagga aatagccaaa aaaggcaaat
      121 agtggaaaaa tagtaaacat taagtaaatg ctgtagaagt gagtttgtaa atattctaca
      181 catgtaaaat atgtaaaact atggggtttt tttattaaat gtattttaaa ataaaaattc
      241 tgtttttttc tgattagact gcaaaagtga gaaaagtcca attctcttga aatgtagaat
      301 tgaaaatgca gtagggaaaa cttaataaaa attattacca gttattttga agatctggcc
      361 cctatagtac cacaa
//
Feature
Display: FASTA GenBank Help
Details

Supplemental Content

Change region shown

Customize view

Recent activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...
External link. Please review our privacy policy.