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GenBank: BU759390.1
FASTA Graphics
LOCUS BU759390 539 bp mRNA linear EST 27-JAN-2011 DEFINITION UI-R-FF0-cph-e-18-0-UI.s1 NCI_CGAP_FF0 Rattus norvegicus cDNA clone UI-R-FF0-cph-e-18-0-UI 3', mRNA sequence. ACCESSION BU759390 VERSION BU759390.1 DBLINK BioSample: SAMN00170794 KEYWORDS EST. SOURCE Rattus norvegicus (Norway rat) ORGANISM Rattus norvegicus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus. REFERENCE 1 (bases 1 to 539) AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B. TITLE Normalization and subtraction: two approaches to facilitate gene discovery JOURNAL Genome Res. 6 (9), 791-806 (1996) PUBMED 8889548 COMMENT Contact: Soares, MB Cancer Biology & Epigenomics Program Children's Memorial Research Center 2300 Children's Plaza, Box 220, Chicago, IL 60614-3394, USA Tel: 773 755 6551 Fax: 773 755 6378 Email: mbsoares@childrensmemorial.org Tissue Procurement: Jeff Stevens, University of Iowa cDNA Library preparation: Dr. M. Bento Soares, University of Iowa cDNA Library Arrayed by: Dr. M. Bento Soares, University of Iowa DNA Sequencing by: Dr. M. Bento Soares, University of Iowa Clone Distribution: DISTRIBUTION: Researchers may obtain clones from Research Genetics (www.resgen.com). Seq primer: M13 FORWARD POLYA=Yes. FEATURES Location/Qualifiers source 1..539 /organism="Rattus norvegicus" /mol_type="mRNA" /db_xref="taxon:10116" /clone="UI-R-FF0-cph-e-18-0-UI" /tissue_type="Mixed tissues" /clone_lib="SAMN00170794 NCI_CGAP_FF0" /dev_stage="Adult" /lab_host="DH10B (Life Technologies) (T1 phage resistant)" /note="Vector: pT7T3-Pac (Pharmacia) with a modified polylinker; Site_1: EcoR I; Site_2: Not I; UI-R-FF0 is a subtracted cDNA library containing the following tissue(s): Normal cartilage and SR-JWS Tumor Line . The subtraction was made according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tags for these libraries are: CTAATGGACG, CATTCTTGTA. TAG_LIB=UI-R-FF0; TAG_SEQ=CTAATGGACG; TAG_TISSUE=cartilage" ORIGIN 1 tttttttttt tttttgttgg agaatgaagg tgttaggtca tatggatatt gtttttacag 61 gcaaatttta ttcccaaatc agcagggcta agcacaccct atgctaacac tgccttctgt 121 gtcttctgat gcagactaag ataaaaataa ggctgaggag gtttaagatt cccactgtgc 181 ctcatccttc tgacattttt gtccttaggt catataaggg tttctgctcc aagccctcca 241 atcaagcatt agctcttccc tgagaaggct agggttaccc tctggtctgc agagaaaccc 301 acctgtacgg ttcaggaaag gctcagcaga gcactttgct tgtccaccct ctcactagac 361 tagaggagct gcctgcctta ggagcagcaa gccttgcttt ttgactaatc ttttattaaa 421 cacatgtgaa cacacagaca ccatggctct tccaagccag gcagaataat ttccagacgt 481 cagttctgcc ccttcaccgt caacaagtca ggttgtcaga cttggcccgc cctcgtgcc //
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