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GenBank: BU615915.1
FASTA Graphics
LOCUS BU615915 672 bp mRNA linear EST 05-JAN-2011 DEFINITION UI-H-DF0-bej-h-21-0-UI.s1 NCI_CGAP_DF0 Homo sapiens cDNA clone UI-H-DF0-bej-h-21-0-UI 3', mRNA sequence. ACCESSION BU615915 VERSION BU615915.1 DBLINK BioSample: SAMN00170290 KEYWORDS EST. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 672) CONSRTM NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap TITLE National Cancer Institute, Cancer Genome Anatomy Project (CGAP), Tumor Gene Index JOURNAL Unpublished COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov Tissue Procurement: Dr. Jose Mercuende cDNA Library preparation: Dr. M. Bento Soares, University of Iowa cDNA Library Arrayed by: Dr. M. Bento Soares, University of Iowa DNA Sequencing by: Dr. M. Bento Soares, University of Iowa Clone Distribution: Clone distribution information can be obtained from Dr. M. Bento Soares, bento-soares@uiowa.edu Seq primer: M13 FORWARD POLYA=Yes. FEATURES Location/Qualifiers source 1..672 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="UI-H-DF0-bej-h-21-0-UI" /tissue_type="Subchondral Bone" /clone_lib="SAMN00170290 NCI_CGAP_DF0" /dev_stage="Adult" /lab_host="DH10B (Life Technologies)" /note="Organ: Bone; Vector: pT7T3-Pac (Pharmacia) with a modified polylinker; Site_1: EcoR I; Site_2: Not I; NCI_CGAP_DF0 is a cDNA library containing the following tissue(s): Subchondral Bone. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is GTTAAGCGTC. TAG_LIB=UI-H-DF0; TAG_SEQ=GTTAAGCGTC; TAG_TISSUE=subchondral bone" ORIGIN 1 tttttttttt ttttttttgt aagtcaatgt gattatttgt ttcatttcag aagatctatg 61 ggtcccactg cccgccacac gtgtctcctg gttctcaacg aagtgtgacc agctcttctg 121 aagaggtagg gtgaatggcg actgtgttgt caaagtctgc cttcgttgct cccatcttca 181 ctgcaacagc aaaaccctgc agcatttcat cacacccaag tccctgcata tggatcccaa 241 ccaccttttc ttccttgtta gcacagacca ttttcatcac acattttgtt ttccttttgg 301 taactgcgtg atacatcggg gtaaagctcg ttgaataggt cttcacattt tctattccat 361 atttatgaat ggcttcatct tccgtgagtc ccactgtccc aatagggggg tggctgaaga 421 ccacagttgg gatgttgtta taatctaatt tggaatcttc cttatattca aaaagtcgat 481 gggcaagttt tcggccagca gctattgcaa ctggagtaag aagagttttc cacatacatc 541 cccaactgca tagatgcctt tgacgttggt attctggaat tcgtctacga tgatatgacc 601 cttgtcatcg gtttgaatcc ccagtttgtt tanactcagg tccttggtat tcgggaccgc 661 ccaatggcca ga //
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