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UI-1-BB1p-aua-h-08-0-UI.s1 NCI_CGAP_Pl6 Homo sapiens cDNA clone UI-1-BB1p-aua-h-08-0-UI 3', mRNA sequence

GenBank: BQ025484.1

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LOCUS       BQ025484                 695 bp    mRNA    linear   EST 07-JAN-2011
DEFINITION  UI-1-BB1p-aua-h-08-0-UI.s1 NCI_CGAP_Pl6 Homo sapiens cDNA clone
            UI-1-BB1p-aua-h-08-0-UI 3', mRNA sequence.
ACCESSION   BQ025484
VERSION     BQ025484.1
DBLINK      BioSample: SAMN00170300
KEYWORDS    EST.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 695)
  CONSRTM   NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap
  TITLE     National Cancer Institute, Cancer Genome Anatomy Project (CGAP),
            Tumor Gene Index
  JOURNAL   Unpublished
COMMENT     Contact: Robert Strausberg, Ph.D.
            Email: cgapbs-r@mail.nih.gov
            Tissue Procurement: Dr. Steven Brown
             cDNA Library preparation: Dr. M. Bento Soares, University of Iowa
             cDNA Library Arrayed by: Dr. M. Bento Soares, University of Iowa
             DNA Sequencing by: Dr. M. Bento Soares, University of Iowa
             Clone Distribution: Clone distribution information can be obtained
            from Dr. M. Bento Soares, bento-soares@uiowa.edu
             The following repetitive elements were found in this cDNA
            sequence: 16-147, >ALU (matched compliment)
            Seq primer: M13 FORWARD
            POLYA=No.
FEATURES             Location/Qualifiers
     source          1..695
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /clone="UI-1-BB1p-aua-h-08-0-UI"
                     /tissue_type="Placenta"
                     /clone_lib="SAMN00170300 NCI_CGAP_Pl6"
                     /dev_stage="Full Term"
                     /lab_host="DH10B (Life Technologies)"
                     /note="Organ: Placenta; Vector: pT7T3-Pac (Pharmacia) with
                     a modified polylinker; Site_1: EcoR I; Site_2: Not I;
                     NCI_CGAP_Pl6 is a subtracted cDNA library constructed
                     according to Bonaldo, Lennon and Soares, Genome Research,
                     6:791-806, 1996. First strand cDNA synthesis was primed
                     with an oligo-dT primer containing a Not I site. Double
                     stranded cDNA was ligated to an EcoR I adaptor, digested
                     with Not I, and cloned directionally into pT7T3-Pac
                     vector. The oligonucleotide used to prime the synthesis of
                     first-strand cDNA contains a library tag sequence that is
                     located between the Not I site and the (dT)18 tail. The
                     sequence tags for this library are GA, AGGAA. For
                     additional information, contact: Bento Soares,
                     bento-soares@uiowa.edu;
                     TAG_LIB=UI-1-BB1p;
                     TAG_SEQ=AGGAA;
                     TAG_TISSUE=placenta human full term"
ORIGIN      
        1 gcggccgcag gaattttttt gtgtctttgg tggggacggg agtttcacca tgttggccag
       61 gctagtctta gactcctgac ctcgtgatct gcctggcttg acctcccggg gtgttgggat
      121 tacaggcgtg agccgttgca cctggccagg gtgtgtctta ttgaaattga acaaaatacc
      181 taatttctag agcgtataag agaagtttaa aatgctttat ggatgtgttg ttttgacagc
      241 aaaatatcta ctcagaatcc tatagctatt tcaaaatcca agtaacttag aaaaaaagga
      301 aaaagaaaac ctatatagtc aaatcttttg gtgattttgt attcaatgac tgaaacttcc
      361 cagtgattat tgggcttttt agctggaatt gaacttgaat cggggcagag cagcacaatg
      421 cttcagaact tcagcgactc tgagccctgg ttctgcaatg acctgccaag tagctttagt
      481 ctacttgact gctctgaacc ttaattttct cacctgtatg ggaatcatag actctacttt
      541 atgaggctga cgtaagcatt acatgaaatt ttgtatactt atacataatg tgcttagcac
      601 cgaatacttg gtgacagcag atgcccaatg ggagttatca cagatattat ttcagaatcg
      661 tggagagtca gaagccacaa attcttgatt tctgt
//
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