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GenBank: BQ025484.1
FASTA Graphics
LOCUS BQ025484 695 bp mRNA linear EST 07-JAN-2011 DEFINITION UI-1-BB1p-aua-h-08-0-UI.s1 NCI_CGAP_Pl6 Homo sapiens cDNA clone UI-1-BB1p-aua-h-08-0-UI 3', mRNA sequence. ACCESSION BQ025484 VERSION BQ025484.1 DBLINK BioSample: SAMN00170300 KEYWORDS EST. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 695) CONSRTM NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap TITLE National Cancer Institute, Cancer Genome Anatomy Project (CGAP), Tumor Gene Index JOURNAL Unpublished COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov Tissue Procurement: Dr. Steven Brown cDNA Library preparation: Dr. M. Bento Soares, University of Iowa cDNA Library Arrayed by: Dr. M. Bento Soares, University of Iowa DNA Sequencing by: Dr. M. Bento Soares, University of Iowa Clone Distribution: Clone distribution information can be obtained from Dr. M. Bento Soares, bento-soares@uiowa.edu The following repetitive elements were found in this cDNA sequence: 16-147, >ALU (matched compliment) Seq primer: M13 FORWARD POLYA=No. FEATURES Location/Qualifiers source 1..695 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="UI-1-BB1p-aua-h-08-0-UI" /tissue_type="Placenta" /clone_lib="SAMN00170300 NCI_CGAP_Pl6" /dev_stage="Full Term" /lab_host="DH10B (Life Technologies)" /note="Organ: Placenta; Vector: pT7T3-Pac (Pharmacia) with a modified polylinker; Site_1: EcoR I; Site_2: Not I; NCI_CGAP_Pl6 is a subtracted cDNA library constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tags for this library are GA, AGGAA. For additional information, contact: Bento Soares, bento-soares@uiowa.edu; TAG_LIB=UI-1-BB1p; TAG_SEQ=AGGAA; TAG_TISSUE=placenta human full term" ORIGIN 1 gcggccgcag gaattttttt gtgtctttgg tggggacggg agtttcacca tgttggccag 61 gctagtctta gactcctgac ctcgtgatct gcctggcttg acctcccggg gtgttgggat 121 tacaggcgtg agccgttgca cctggccagg gtgtgtctta ttgaaattga acaaaatacc 181 taatttctag agcgtataag agaagtttaa aatgctttat ggatgtgttg ttttgacagc 241 aaaatatcta ctcagaatcc tatagctatt tcaaaatcca agtaacttag aaaaaaagga 301 aaaagaaaac ctatatagtc aaatcttttg gtgattttgt attcaatgac tgaaacttcc 361 cagtgattat tgggcttttt agctggaatt gaacttgaat cggggcagag cagcacaatg 421 cttcagaact tcagcgactc tgagccctgg ttctgcaatg acctgccaag tagctttagt 481 ctacttgact gctctgaacc ttaattttct cacctgtatg ggaatcatag actctacttt 541 atgaggctga cgtaagcatt acatgaaatt ttgtatactt atacataatg tgcttagcac 601 cgaatacttg gtgacagcag atgcccaatg ggagttatca cagatattat ttcagaatcg 661 tggagagtca gaagccacaa attcttgatt tctgt //
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