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UI-H-EI1-ayv-d-06-0-UI.s1 NCI_CGAP_EI1 Homo sapiens cDNA clone IMAGE:5844053 3', mRNA sequence

GenBank: BQ002893.1

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LOCUS       BQ002893                 564 bp    mRNA    linear   EST 04-JAN-2011
DEFINITION  UI-H-EI1-ayv-d-06-0-UI.s1 NCI_CGAP_EI1 Homo sapiens cDNA clone
            IMAGE:5844053 3', mRNA sequence.
ACCESSION   BQ002893
VERSION     BQ002893.1
DBLINK      BioSample: SAMN00153784
KEYWORDS    EST.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 564)
  CONSRTM   NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap
  TITLE     National Cancer Institute, Cancer Genome Anatomy Project (CGAP),
            Tumor Gene Index
  JOURNAL   Unpublished
COMMENT     Contact: Robert Strausberg, Ph.D.
            Email: cgapbs-r@mail.nih.gov
            Tissue Procurement: Dr. Jose Mercuende
             cDNA Library preparation: Dr. M. Bento Soares, University of Iowa
             cDNA Library Arrayed by: Dr. M. Bento Soares, University of Iowa
             DNA Sequencing by: Dr. M. Bento Soares, University of Iowa
             Clone Distribution: Clone distribution information can be found
            through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
             The following repetitive elements were found in this cDNA
            sequence: 1-69, >AT_rich#Low_complexity (matched compliment)
            288-344, >MIR#SINE/MIR (matched compliment)
            Seq primer: M13 FORWARD
            POLYA=Yes.
FEATURES             Location/Qualifiers
     source          1..564
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /clone="IMAGE:5844053"
                     /tissue_type="Chondrosarcoma"
                     /clone_lib="SAMN00153784 NCI_CGAP_EI1"
                     /dev_stage="Adult"
                     /lab_host="DH10B (Life Technologies)"
                     /note="Organ: Left Pelvis; Vector: pT7T3-Pac (Pharmacia)
                     with a modified polylinker; Site_1: EcoR I; Site_2: Not I;
                     NCI_CGAP_EI1 is a normalized cDNA library containing the
                     following tissue(s): Chondrosarcoma. The library was
                     constructed according to Bonaldo, Lennon and Soares,
                     Genome Research, 6:791-806, 1996. First strand cDNA
                     synthesis was primed with an oligo-dT primer containing a
                     Not I site. Double stranded cDNA was ligated to an EcoR I
                     adaptor, digested with Not I, and cloned directionally
                     into pT7T3-Pac vector. The oligonucleotide used to prime
                     the synthesis of first-strand cDNA contains a library tag
                     sequence that is located between the Not I site and the
                     (dT)18 tail. The sequence tag for this library is
                     ACACTTGCAC.
                     TAG_LIB=UI-H-EI1;
                     TAG_SEQ=ACACTTGCAC;
                     TAG_TISSUE=chondrosarcoma"
ORIGIN      
        1 tttttttttt ttttttttac atccacaatt ttatttaata agtatatcat taacattttc
       61 ttaatatatc agaaaagatg aaaagaaaca atatagaaat attttcagct atacaaattc
      121 agggcttcca tgacaaataa aggctaaaga ttccaccttt atttacatac atttttttct
      181 tcatagaaaa agtcgatatc caataaaatg ttacctaatg taaccccagg caaagtgaat
      241 gccatgactg gacaatggag acttattcgt tgaaccgtta cacctaatta ccatgtgcca
      301 gatactgttc taagcactct atggatgtta gctcatttaa tcttaaaacc atgtcataga
      361 atatggtaaa aaataaaaat gaaaaaacta aaattgctac ttttctcccc acctcttcat
      421 cctaccccat catatattgt cagtaaatgg acagcaggag tccaatgaaa atatatttaa
      481 cttggactca aaagatttga gttagtttct ggctctattt agggttctgt gtgaccttga
      541 acaagttatt gaaaccagaa cttc
//
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