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GenBank: BQ002893.1
FASTA Graphics
LOCUS BQ002893 564 bp mRNA linear EST 04-JAN-2011 DEFINITION UI-H-EI1-ayv-d-06-0-UI.s1 NCI_CGAP_EI1 Homo sapiens cDNA clone IMAGE:5844053 3', mRNA sequence. ACCESSION BQ002893 VERSION BQ002893.1 DBLINK BioSample: SAMN00153784 KEYWORDS EST. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 564) CONSRTM NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap TITLE National Cancer Institute, Cancer Genome Anatomy Project (CGAP), Tumor Gene Index JOURNAL Unpublished COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov Tissue Procurement: Dr. Jose Mercuende cDNA Library preparation: Dr. M. Bento Soares, University of Iowa cDNA Library Arrayed by: Dr. M. Bento Soares, University of Iowa DNA Sequencing by: Dr. M. Bento Soares, University of Iowa Clone Distribution: Clone distribution information can be found through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov The following repetitive elements were found in this cDNA sequence: 1-69, >AT_rich#Low_complexity (matched compliment) 288-344, >MIR#SINE/MIR (matched compliment) Seq primer: M13 FORWARD POLYA=Yes. FEATURES Location/Qualifiers source 1..564 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="IMAGE:5844053" /tissue_type="Chondrosarcoma" /clone_lib="SAMN00153784 NCI_CGAP_EI1" /dev_stage="Adult" /lab_host="DH10B (Life Technologies)" /note="Organ: Left Pelvis; Vector: pT7T3-Pac (Pharmacia) with a modified polylinker; Site_1: EcoR I; Site_2: Not I; NCI_CGAP_EI1 is a normalized cDNA library containing the following tissue(s): Chondrosarcoma. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is ACACTTGCAC. TAG_LIB=UI-H-EI1; TAG_SEQ=ACACTTGCAC; TAG_TISSUE=chondrosarcoma" ORIGIN 1 tttttttttt ttttttttac atccacaatt ttatttaata agtatatcat taacattttc 61 ttaatatatc agaaaagatg aaaagaaaca atatagaaat attttcagct atacaaattc 121 agggcttcca tgacaaataa aggctaaaga ttccaccttt atttacatac atttttttct 181 tcatagaaaa agtcgatatc caataaaatg ttacctaatg taaccccagg caaagtgaat 241 gccatgactg gacaatggag acttattcgt tgaaccgtta cacctaatta ccatgtgcca 301 gatactgttc taagcactct atggatgtta gctcatttaa tcttaaaacc atgtcataga 361 atatggtaaa aaataaaaat gaaaaaacta aaattgctac ttttctcccc acctcttcat 421 cctaccccat catatattgt cagtaaatgg acagcaggag tccaatgaaa atatatttaa 481 cttggactca aaagatttga gttagtttct ggctctattt agggttctgt gtgaccttga 541 acaagttatt gaaaccagaa cttc //
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