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GenBank: BM986967.1
FASTA Graphics
LOCUS BM986967 347 bp mRNA linear EST 08-JAN-2011 DEFINITION UI-H-CO0-aqe-e-12-0-UI.s1 NCI_CGAP_Sub9 Homo sapiens cDNA clone IMAGE:3103941 3', mRNA sequence. ACCESSION BM986967 VERSION BM986967.1 DBLINK BioSample: SAMN00170302 KEYWORDS EST. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 347) CONSRTM NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap TITLE National Cancer Institute, Cancer Genome Anatomy Project (CGAP), Tumor Gene Index JOURNAL Unpublished COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov Tissue Procurement: Dr. Jose Mercuende cDNA Library preparation: Dr. M. Bento Soares, University of Iowa cDNA Library Arrayed by: Dr. M. Bento Soares, University of Iowa DNA Sequencing by: Dr. M. Bento Soares, University of Iowa Clone Distribution: Clone distribution information can be found through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov Seq primer: M13 FORWARD POLYA=Yes. FEATURES Location/Qualifiers source 1..347 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="IMAGE:3103941" /tissue_type="mixed" /clone_lib="SAMN00170302 NCI_CGAP_Sub9" /dev_stage="mixed" /lab_host="DH10B (Life Technologies)" /note="Vector: pT7T3-Pac (Pharmacia) with a modified polylinker; Site_1: EcoR I; Site_2: Not I; tissues: Cholonic mucosa with Crohns disease, Cholonic mucosa with ulcerative colitis, Fetal thymus, Cervix, Cervical adenosquamous carcinoma, Ligament cells, Prostate carcinoma, Bladder carcinoma, Brain oligodenroga; NCI_CGAP_Sub9 is a subtracted cDNA library constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tags for this library are CGTC, AACG, GGGCC, GGAAG, TAGC, TAAGC, ATGG, AGACA, ATCAC. For additional information, contact: Bento Soares, bento-soares@uiowa.edu; TAG_LIB=UI-H-CO0; TAG_SEQ=GGGCC; TAG_TISSUE=Cervix" ORIGIN 1 tttttttttt ttttttatag gtgggatgag ttaattaatc tcaaacacat ttaaattaac 61 aggaagaaag aaatagggca tggtaggagg agagaaagga ggacagttag ggatatagtg 121 aatactttta aagtcagttg aattatttca tacgggataa tttttcttgt cacagtaaat 181 caccctgtgc cccacccccg ctccaggaag gcagggaggt tgtaaaggag aagcgagaag 241 agggttaatt tgagctccct cctgcctgct gcagtcacag tgcacgataa agtaatgaag 301 gtgacgccgc cacatatctc atctgaactt cctctgtggg ctttttc //
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