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UI-H-CO0-aqe-e-12-0-UI.s1 NCI_CGAP_Sub9 Homo sapiens cDNA clone IMAGE:3103941 3', mRNA sequence

GenBank: BM986967.1

FASTA Graphics 

LOCUS       BM986967                 347 bp    mRNA    linear   EST 08-JAN-2011
DEFINITION  UI-H-CO0-aqe-e-12-0-UI.s1 NCI_CGAP_Sub9 Homo sapiens cDNA clone
            IMAGE:3103941 3', mRNA sequence.
ACCESSION   BM986967
VERSION     BM986967.1
DBLINK      BioSample: SAMN00170302
KEYWORDS    EST.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 347)
  CONSRTM   NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap
  TITLE     National Cancer Institute, Cancer Genome Anatomy Project (CGAP),
            Tumor Gene Index
  JOURNAL   Unpublished
COMMENT     Contact: Robert Strausberg, Ph.D.
            Email: cgapbs-r@mail.nih.gov
            Tissue Procurement: Dr. Jose Mercuende
             cDNA Library preparation: Dr. M. Bento Soares, University of Iowa
             cDNA Library Arrayed by: Dr. M. Bento Soares, University of Iowa
             DNA Sequencing by: Dr. M. Bento Soares, University of Iowa
             Clone Distribution: Clone distribution information can be found
            through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
            
            Seq primer: M13 FORWARD
            POLYA=Yes.
FEATURES             Location/Qualifiers
     source          1..347
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /clone="IMAGE:3103941"
                     /tissue_type="mixed"
                     /clone_lib="SAMN00170302 NCI_CGAP_Sub9"
                     /dev_stage="mixed"
                     /lab_host="DH10B (Life Technologies)"
                     /note="Vector: pT7T3-Pac (Pharmacia) with a modified
                     polylinker; Site_1: EcoR I; Site_2: Not I; tissues:
                     Cholonic mucosa with Crohns disease, Cholonic mucosa with
                     ulcerative colitis, Fetal thymus, Cervix, Cervical
                     adenosquamous carcinoma, Ligament cells, Prostate
                     carcinoma, Bladder carcinoma, Brain oligodenroga;
                     NCI_CGAP_Sub9 is a subtracted cDNA library constructed
                     according to Bonaldo, Lennon and Soares, Genome Research,
                     6:791-806, 1996. First strand cDNA synthesis was primed
                     with an oligo-dT primer containing a Not I site. Double
                     stranded cDNA was ligated to an EcoR I adaptor, digested
                     with Not I, and cloned directionally into pT7T3-Pac
                     vector. The oligonucleotide used to prime the synthesis of
                     first-strand cDNA contains a library tag sequence that is
                     located between the Not I site and the (dT)18 tail. The
                     sequence tags for this library are CGTC, AACG, GGGCC,
                     GGAAG, TAGC, TAAGC, ATGG, AGACA, ATCAC. For additional
                     information, contact: Bento Soares,
                     bento-soares@uiowa.edu;
                     TAG_LIB=UI-H-CO0;
                     TAG_SEQ=GGGCC;
                     TAG_TISSUE=Cervix"
ORIGIN      
        1 tttttttttt ttttttatag gtgggatgag ttaattaatc tcaaacacat ttaaattaac
       61 aggaagaaag aaatagggca tggtaggagg agagaaagga ggacagttag ggatatagtg
      121 aatactttta aagtcagttg aattatttca tacgggataa tttttcttgt cacagtaaat
      181 caccctgtgc cccacccccg ctccaggaag gcagggaggt tgtaaaggag aagcgagaag
      241 agggttaatt tgagctccct cctgcctgct gcagtcacag tgcacgataa agtaatgaag
      301 gtgacgccgc cacatatctc atctgaactt cctctgtggg ctttttc
//
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