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UI-E-EJ0-ahm-h-15-0-UI.s1 UI-E-EJ0 Homo sapiens cDNA clone UI-E-EJ0-ahm-h-15-0-UI 3', mRNA sequence

GenBank: BM674836.1

FASTA Graphics 

LOCUS       BM674836                 485 bp    mRNA    linear   EST 26-JAN-2011
DEFINITION  UI-E-EJ0-ahm-h-15-0-UI.s1 UI-E-EJ0 Homo sapiens cDNA clone
            UI-E-EJ0-ahm-h-15-0-UI 3', mRNA sequence.
ACCESSION   BM674836
VERSION     BM674836.1
DBLINK      BioSample: SAMN00170185
KEYWORDS    EST.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Bonaldo,M.F., Lennon,G. and Soares,M.B.
  TITLE     Normalization and subtraction: two approaches to facilitate gene
            discovery
  JOURNAL   Genome Res. 6 (9), 791-806 (1996)
   PUBMED   8889548
COMMENT     Contact: Soares, MB
            Cancer Biology & Epigenomics Program
            Children's Memorial Research Center
            2300 Children's Plaza, Box 220, Chicago, IL 60614-3394, USA
            Tel: 773 755 6551
            Fax: 773 755 6378
            Email: mbsoares@childrensmemorial.org
            Tissue Procurement: Dr. Gregg Hageman
             cDNA Library preparation: Dr. M. Bento Soares, Univeristy of Iowa
             cDNA Library Arrayed by: Dr. M. Bento Soares, Univeristy of Iowa
             DNA Sequencing by: Dr. M. Bento Soares, Univeristy of Iowa
             Clone Distribution: Researchers may obtain clones from Research
            Genetics (www.resgen.com).
            
            Seq primer: M13 Forward
            POLYA=Yes.
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /clone="UI-E-EJ0-ahm-h-15-0-UI"
                     /tissue_type="fetal eyes, lens, eye anterior segment,
                     optic nerve, retina, Retina Foveal and Macular, RPE and
                     Choroid"
                     /clone_lib="SAMN00170185 UI-E-EJ0"
                     /dev_stage="fetal and adult"
                     /lab_host="DH10B (Life Technologies) (T1 phage resistant)"
                     /note="Organ: eye; Vector: pT7T3-Pac (Pharmacia) with a
                     modified polylinker; Site_1: EcoR I; Site_2: Not I;
                     UI-E-EJ0 is a subtracted cDNA library constructed
                     according to Bonaldo, Lennon and Soares, Genome Research,
                     6:791-806, 1996. First strand cDNA synthesis was primed
                     with an oligo-dT primer containing a Not I site. Double
                     stranded cDNA was ligated to an EcoR I adaptor, digested
                     with Not I, and cloned directionally into pT7T3-Pac
                     vector. The oligonucleotide used to prime the synthesis of
                     first-strand cDNA contains a library tag sequence that is
                     located between the Not I site and the (dT)18 tail. The
                     sequence tags for this library are: fetal eyes,
                     AGAATCAAGA; lens, CGATTAGCGA; eye anterior segment,
                     AATGCCGCAT; optic nerve, CCATTAAGTG; retina, CCGCG; Retina
                     Foveal and Macular, GTCC; RPE and Choroid, ACCTA. This
                     library was created for the program, Gene Discovery in the
                     Visual System, supported by National Eye Institute (NEI).
                     TAG_LIB=UI-E-EJ0;
                     TAG_SEQ=CCGCG;
                     TAG_TISSUE=human retina"
ORIGIN      
        1 tttttttttt tttttctgtt ctgccttcca cttgtcagtt ccatccttat tctgcttcct
       61 ttagggatct ctaggcactg ccaacactgt caggactcta gacttcctaa cgactagagg
      121 aagaaagact agtttacata tgggcgcttc cttatggagc taaggaaatt tctttctcaa
      181 aagcccacaa tcactttctc gttgtgtctc attgcccaaa ttgggtcata ggactgtctg
      241 taagcatggg ctaggagatg ggataatgct gattactata tgccacattc ccctacccct
      301 ggagatacag aggattcaac atctctccaa gctgatgaga tgcactgggg acatatgaat
      361 ggttgaatta aaatgagaga taccatgaga aagaatacca tgagaaagaa tagataatac
      421 catgagaaag aatagataat accatgagaa agaatagata atgggaacca aaccaccctc
      481 gtgcc
//
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