LOCUS BM037052 471 bp mRNA linear EST 05-NOV-2001
DEFINITION fu82d05.y1 Gong zebrafish ovary Danio rerio cDNA clone
IMAGE:5377568 5', mRNA sequence.
ACCESSION BM037052
VERSION BM037052.1
DBLINK BioSample: SAMN00164273
KEYWORDS EST.
SOURCE Danio rerio (zebrafish)
ORGANISM Danio rerio
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Actinopterygii; Neopterygii; Teleostei; Ostariophysi;
Cypriniformes; Danionidae; Danioninae; Danio.
REFERENCE 1 (bases 1 to 471)
AUTHORS Clark,M., Johnson,S.L., Lehrach,H., Lee,R., Li,F., Marra,M.,
Eddy,S., Hillier,L., Kucaba,T., Martin,J., Beck,C., Wylie,T.,
Underwood,K., Steptoe,M., Theising,B., Allen,M., Bowers,Y.,
Person,B., Swaller,T., Gibbons,M., Pape,D., Harvey,N., Schurk,R.,
Ritter,E., Kohn,S., Shin,T., Jackson,Y., Cardenas,M., McCann,R.,
Waterston,R. and Wilson,R.
TITLE WashU Zebrafish EST Project 1998
JOURNAL Unpublished
COMMENT Other_ESTs: fu82d05.x1
Contact: Stephen L. Johnson
Washington University School of Medicine
4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
Tel: 314 286 1800
Fax: 314 286 1810
Email: zbrafish@watson.wustl.edu
The library was constructed by Dr. Z. Gong. DNA Sequencing by:
Washington University Genome Sequencing Center St. Louis. Please
contact Zhiyuan Gong for further information on this library
(National University of Singapore, Department of Biological
Sciences, Lower Kent Ridge Road, Singapore 119260).
High quality sequence stop: 398.
FEATURES Location/Qualifiers
source 1..471
/organism="Danio rerio"
/mol_type="mRNA"
/db_xref="taxon:7955"
/clone="IMAGE:5377568"
/sex="female"
/clone_lib="SAMN00164273 Gong zebrafish ovary"
/dev_stage="4-5 month"
/lab_host="DH10B (phage-resistant)"
/note="Organ: ovary (pooled); Vector: pBluescript SK-;
Site_1: XhoI; Site_2: EcoRI; Poly A+ RNA was isolatd from
the ovaries of 2 female adult zebrafish (4-5 month old).
cDNAs were made using oligo-dT primers and inserted into
lambda ZAP II vector (Stratagene) by Dr. Z. Gong, in vivo
mass-excised to pBluescript SK- following the Washington
University protocol
(http://genome.wustl.edu/est/lambda_protocol.shtml).
Please contact Zhiyuan Gong for further information on
this library (National University of Singapore, Department
of Biological Sciences, Lower Kent Ridge Road, Singapore
119260)."
ORIGIN
1 actcatgcaa aaagatggac aggatttctc agtgtatctt tcggatggac tgcatctgtc
61 tgataaaggc aatcagtttg tggcagaaca tttatggacg cttctagaga ggcgagtgtc
121 tgatttgccc ttcattctgc cttattgggg ggacgtggac ccaaaatgcc ctgagagcag
181 cctactgtgc gactaaaact caggtcaaat gatgcataaa ggatataaaa cagcattgtg
241 tcccttttgc ttacatttag acatgtttgg atatgatgaa gatcatgaaa ggctcagcca
301 ccatgttcac ttatcctttt tcaactgaaa caaactgtat ctaaatacag gaggtattta
361 cagttacaaa tgcaagctga atgactgata caaaaatgtt tgatgttgaa aatagtcctt
421 tttttaaaac ggtttaataa agttttgatg aagaaaatgg aaaaaaaaaa a
//