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GenBank: BI290708.1
FASTA Graphics
LOCUS BI290708 375 bp mRNA linear EST 26-JAN-2011 DEFINITION UI-R-DK0-cfy-e-01-0-UI.s1 UI-R-DK0 Rattus norvegicus cDNA clone UI-R-DK0-cfy-e-01-0-UI 3', mRNA sequence. ACCESSION BI290708 VERSION BI290708.1 DBLINK BioSample: SAMN00164153 KEYWORDS EST. SOURCE Rattus norvegicus (Norway rat) ORGANISM Rattus norvegicus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus. REFERENCE 1 (bases 1 to 375) AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B. TITLE Normalization and subtraction: two approaches to facilitate gene discovery JOURNAL Genome Res. 6 (9), 791-806 (1996) PUBMED 8889548 COMMENT Contact: Soares, MB Cancer Biology & Epigenomics Program Children's Memorial Research Center 2300 Children's Plaza, Box 220, Chicago, IL 60614-3394, USA Tel: 773 755 6551 Fax: 773 755 6378 Email: mbsoares@childrensmemorial.org The sequence contained an oligo-dT track that was present in the oligonucleotide that was used to prime the synthesis of first strand cDNA and therefore this may represent a bonafide poly A tail. The sequence tag present in the cDNA between the NotI site and the oligo-dT track served to identify it as a clone from the normalized rat heart pool library cDNA Library Preparation: M.B. Soares Lab Clone distribution: clones will be available through Research Genetics (www.resgen.com) The following repetitive elements were found in this cDNA sequence: 1-23, >AT_rich#Low_complexity Seq primer: M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..375 /organism="Rattus norvegicus" /mol_type="mRNA" /strain="Sprague-Dawley" /db_xref="taxon:10116" /clone="UI-R-DK0-cfy-e-01-0-UI" /clone_lib="SAMN00164153 UI-R-DK0" /dev_stage="ADULT" /lab_host="DH10B (Life Technologies)" /note="Vector: pT7T3D-PacI; Site_1: Not I; Site_2: Eco RI; The UI-R-DK0 library is a subtracted library derived from a mixture of five individually tagged normalized rat libraries: brain-nRBP (20%), heart-nRHP (20%), kidney-nRKP (20%), aorta-nRAP (20%), and placenta-nRPP (20%). Each original library was constructed from a mixture of equal amounts of RNA from seven different developmental time-points: embryonic day 17, embryonic day 19, embryonic day 21, adult day 1, adult day 12, adult day 75, and adult day 200. (Exception: the aorta pool does not contain embryonic day 17 RNA and the placenta pool contains only the three embryonic stages). Each library was normalized individually according to the procedure described by Bonaldo, Lennon & Soares (Genome Research Genome 6: 791-806, 1996). For construction of the DK0 subtracted library, plasmid DNA from each of the five individually tagged normalized libraries was mixed in the proportions specified above and electroporated into competent bacteria for production of single-stranded circular DNA representing the pool of libraries. Single-stranded circular DNA representing these five normalized libraries was then used as a tracer in a subtractive hybridization with a driver (PCR amplified inserts from a plasmid DNA template preparation) comprising: a) a set of about 1,000 arrayed clones from each of the five non-normalized libraries of brain (CT0s), heart (CS0s), kidney (CU0s), aorta (CW0s), and placenta (CX0s). The resulting pool of approximately 5,000 clones represented about 33.3% of the final driver population. A set of about 2,000 arrayed clones from each of the five normalized libraries of brain (CT0), heart (CS0), kidney (CU0), aorta (CW0), and placenta (CX0). The resulting pool of about 10,000 clones represented about 66.6% of the final driver population. TAG_LIB=UI-R-DK0; TAG_SEQ=ATAAGATAAC; TAG_TISSUE=rat heart pool" ORIGIN 1 tttttttttt ttttttttaa tttcactgcc agttttatgt tccagaatgt anaacgagaa 61 gcagttacac ctcaggaaaa ttctcagtgc ccttggagaa ttggctgacc tgctctgccc 121 tataatgtcc tgtttatctg gctggacaga gaaaagggta gacaccttta gcttgggaag 181 aaccttcatt ctgccactca ggagtgatga ggtgcctcca accttctctg tagccatcta 241 acaatggtta gagcaagcct ggcatcagtt catgctcacc agaggttaat ttttgggacc 301 aagtacaaaa tctttgggtg gacctccacc accactatgt ctctctgttg tttgggagcc 361 agagaggggc tgcac //
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