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UI-R-DK0-cfy-e-01-0-UI.s1 UI-R-DK0 Rattus norvegicus cDNA clone UI-R-DK0-cfy-e-01-0-UI 3', mRNA sequence

GenBank: BI290708.1

FASTA Graphics 

LOCUS       BI290708                 375 bp    mRNA    linear   EST 26-JAN-2011
DEFINITION  UI-R-DK0-cfy-e-01-0-UI.s1 UI-R-DK0 Rattus norvegicus cDNA clone
            UI-R-DK0-cfy-e-01-0-UI 3', mRNA sequence.
ACCESSION   BI290708
VERSION     BI290708.1
DBLINK      BioSample: SAMN00164153
KEYWORDS    EST.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Bonaldo,M.F., Lennon,G. and Soares,M.B.
  TITLE     Normalization and subtraction: two approaches to facilitate gene
            discovery
  JOURNAL   Genome Res. 6 (9), 791-806 (1996)
   PUBMED   8889548
COMMENT     Contact: Soares, MB
            Cancer Biology & Epigenomics Program
            Children's Memorial Research Center
            2300 Children's Plaza, Box 220, Chicago, IL 60614-3394, USA
            Tel: 773 755 6551
            Fax: 773 755 6378
            Email: mbsoares@childrensmemorial.org
            The sequence contained an oligo-dT track that was present in the
            oligonucleotide that was used to prime the synthesis of first
            strand cDNA and therefore this may represent a bonafide poly A
            tail. The sequence tag present in the cDNA between the NotI site
            and the oligo-dT track served to identify it as a clone from the
            normalized rat heart pool library cDNA Library Preparation: M.B.
            Soares Lab Clone distribution: clones will be available through
            Research Genetics (www.resgen.com) The following repetitive
            elements were found in this cDNA sequence: 1-23,
            >AT_rich#Low_complexity
            Seq primer: M13 Forward
            POLYA=Yes.
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="Sprague-Dawley"
                     /db_xref="taxon:10116"
                     /clone="UI-R-DK0-cfy-e-01-0-UI"
                     /clone_lib="SAMN00164153 UI-R-DK0"
                     /dev_stage="ADULT"
                     /lab_host="DH10B (Life Technologies)"
                     /note="Vector: pT7T3D-PacI; Site_1: Not I; Site_2: Eco RI;
                     The UI-R-DK0 library is a subtracted library derived from
                     a mixture of five individually tagged normalized rat
                     libraries: brain-nRBP (20%), heart-nRHP (20%), kidney-nRKP
                     (20%), aorta-nRAP (20%), and placenta-nRPP (20%). Each
                     original library was constructed from a mixture of equal
                     amounts of RNA from seven different developmental
                     time-points: embryonic day 17, embryonic day 19, embryonic
                     day 21, adult day 1, adult day 12, adult day 75, and adult
                     day 200. (Exception: the aorta pool does not contain
                     embryonic day 17 RNA and the placenta pool contains only
                     the three embryonic stages). Each library was normalized
                     individually according to the procedure described by
                     Bonaldo, Lennon & Soares (Genome Research Genome 6:
                     791-806, 1996). For construction of the DK0 subtracted
                     library, plasmid DNA from each of the five individually
                     tagged normalized libraries was mixed in the proportions
                     specified above and electroporated into competent bacteria
                     for production of single-stranded circular DNA
                     representing the pool of libraries. Single-stranded
                     circular DNA representing these five normalized libraries
                     was then used as a tracer in a subtractive hybridization
                     with a driver (PCR amplified inserts from a plasmid DNA
                     template preparation) comprising: a) a set of about 1,000
                     arrayed clones from each of the five non-normalized
                     libraries of brain (CT0s), heart (CS0s), kidney (CU0s),
                     aorta (CW0s), and placenta (CX0s). The resulting pool of
                     approximately 5,000 clones represented about 33.3% of the
                     final driver population. A set of about 2,000 arrayed
                     clones from each of the five normalized libraries of brain
                     (CT0), heart (CS0), kidney (CU0), aorta (CW0), and
                     placenta (CX0). The resulting pool of about 10,000 clones
                     represented about 66.6% of the final driver population.
                     TAG_LIB=UI-R-DK0;
                     TAG_SEQ=ATAAGATAAC;
                     TAG_TISSUE=rat heart pool"
ORIGIN      
        1 tttttttttt ttttttttaa tttcactgcc agttttatgt tccagaatgt anaacgagaa
       61 gcagttacac ctcaggaaaa ttctcagtgc ccttggagaa ttggctgacc tgctctgccc
      121 tataatgtcc tgtttatctg gctggacaga gaaaagggta gacaccttta gcttgggaag
      181 aaccttcatt ctgccactca ggagtgatga ggtgcctcca accttctctg tagccatcta
      241 acaatggtta gagcaagcct ggcatcagtt catgctcacc agaggttaat ttttgggacc
      301 aagtacaaaa tctttgggtg gacctccacc accactatgt ctctctgttg tttgggagcc
      361 agagaggggc tgcac
//
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