LOCUS       BG378029                 535 bp    mRNA    linear   EST 27-JAN-2011
DEFINITION  UI-R-CT0-bvn-a-05-0-UI.s1 UI-R-CT0 Rattus norvegicus cDNA clone
            UI-R-CT0-bvn-a-05-0-UI 3', mRNA sequence.
ACCESSION   BG378029
VERSION     BG378029.1
DBLINK      BioSample: SAMN00163259
KEYWORDS    EST.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 535)
  AUTHORS   Bonaldo,M.F., Lennon,G. and Soares,M.B.
  TITLE     Normalization and subtraction: two approaches to facilitate gene
            discovery
  JOURNAL   Genome Res. 6 (9), 791-806 (1996)
   PUBMED   8889548
COMMENT     Contact: Soares, MB
            Cancer Biology & Epigenomics Program
            Children's Memorial Research Center
            2300 Children's Plaza, Box 220, Chicago, IL 60614-3394, USA
            Tel: 773 755 6551
            Fax: 773 755 6378
            Email: mbsoares@childrensmemorial.org
            The sequence contained an oligo-dT track that was present in the
            oligonucleotide that was used to prime the synthesis of first
            strand cDNA and therefore this may represent a bonafide poly A
            tail. The sequence tag present in the cDNA between the NotI site
            and the oligo-dT track served to verify it as a clone from the
            normalized rat brain pool library cDNA Library Preparation: M.B.
            Soares Lab Clone distribution: clones will be available through
            Research Genetics (www.resgen.com)
            Seq primer: M13 Forward
            POLYA=Yes.
FEATURES             Location/Qualifiers
     source          1..535
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="Sprague-Dawley"
                     /db_xref="taxon:10116"
                     /clone="UI-R-CT0-bvn-a-05-0-UI"
                     /clone_lib="SAMN00163259 UI-R-CT0"
                     /dev_stage="ADULT"
                     /lab_host="DH10B (Life Technologies)"
                     /note="Vector: pT7T3D-PacI; Site_1: Not I; Site_2: Eco RI;
                     The UI-R-CT0 library is a normalized library constructed
                     from the following rat brain tissues: embryonic day 17,
                     embryonic day 19, embryonic day 21, adult day 1, adult day
                     12, adult day 75, adult day 200. For a detailed
                     description of the library from which this clone was
                     derived, please visit our web site at
                     ratest.eng.uiowa.edu. The subtraction has been previously
                     described in (Bonaldo, Lennon and Soares, Genome Research
                     6:791-806, 1996);
                     TAG_LIB=UI-R-CT0;
                     TAG_SEQ=ACTTC;
                     TAG_TISSUE=rat brain pool"
ORIGIN      
        1 tttttttttt ttttttttaa ggggtgagag tttattggga ggaaggtaac atagttggtc
       61 aaaggtcatc agtgcagttt tgcccttccc ttagggacag ggcaagtcag tccagtgtgt
      121 ggtccttgga gccaaggggg aaggcccagg gagtgtcaca gtatagcagc aggaataata
      181 gtgtgagctc ccccacaggc ccacccacaa cccagtctag gaaccggggt tccctgtcaa
      241 ggtcctcagg catctgctac tgagccccag tctcctgagg cagaaagcgt cgttgccgga
      301 gtgtggactt ctcctcttcc acctctgagg cagtgctagg gctggcaccc tcaggagagc
      361 ccccttcatc ccttgagcag tcaggtcncg catcttcctc atactctgtc aagcaatccg
      421 actcctcccc atcagcacag ccatcagaga cgtagcttgt tgggggctca atccgggaca
      481 cgatctcagc caagtcagtg aagccagagc tcgggaaggc aagtatatct gcacg
//