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UI-R-C0-ig-b-01-0-UI.r1 UI-R-C0 Rattus norvegicus cDNA clone UI-R-C0-ig-b-01-0-UI 5', mRNA sequence

GenBank: BF551250.1

FASTA Graphics 

LOCUS       BF551250                 554 bp    mRNA    linear   EST 26-JAN-2011
DEFINITION  UI-R-C0-ig-b-01-0-UI.r1 UI-R-C0 Rattus norvegicus cDNA clone
            UI-R-C0-ig-b-01-0-UI 5', mRNA sequence.
ACCESSION   BF551250
VERSION     BF551250.1
DBLINK      BioSample: SAMN00155877
KEYWORDS    EST.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 554)
  AUTHORS   Bonaldo,M.F., Lennon,G. and Soares,M.B.
  TITLE     Normalization and subtraction: two approaches to facilitate gene
            discovery
  JOURNAL   Genome Res. 6 (9), 791-806 (1996)
   PUBMED   8889548
COMMENT     Contact: Soares, MB
            Cancer Biology & Epigenomics Program
            Children's Memorial Research Center
            2300 Children's Plaza, Box 220, Chicago, IL 60614-3394, USA
            Tel: 773 755 6551
            Fax: 773 755 6378
            Email: mbsoares@childrensmemorial.org
            cDNA Library Preparation: M.B. Soares Lab Clone distribution:
            clones will be available through Research Genetics (www.resgen.com)
            This clone is also available through the I.M.A.G.E. Consortium at
            LLNL (info@image.llnl.gov). IMAGE ID= 1774183
            Seq primer: M13 Forward.
FEATURES             Location/Qualifiers
     source          1..554
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="Sprague-Dawley"
                     /db_xref="taxon:10116"
                     /clone="UI-R-C0-ig-b-01-0-UI"
                     /clone_lib="SAMN00155877 UI-R-C0"
                     /dev_stage="adult"
                     /lab_host="DH10B (Life Technologies)"
                     /note="Vector: pT7T3D-PacI; Site_1: Not I; Site_2: Eco RI;
                     The UI-R-C0 library is a subtracted library derived from
                     the UI-R-A1 and UI-R-E1 libraries. The UI-R-A1 library
                     consisted of a mixture of individually tagged normalized
                     libraries constructed from rat placenta, adult lung,
                     brain, liver, kidney, heart, spleen, ovary, and muscle.
                     The UI-R-E1 library consisted of a mixture of individually
                     tagged normalized libraries constructed from 8, 12 and
                     18-day embryo. The tag is a string of 3-5 nucleotides
                     present between the Not I site and the oligo-dT track
                     which allows identification of the library of origin of a
                     clone within the mixture. The subtracted library (UI-R-C0)
                     was constructed as follows: PCR amplified cDNA inserts
                     from a pool of UI-R-A1 and UI-R-E1 clones from which 3'
                     ESTs had been derived was used as a driver in a
                     hybridization with the pooled UI-R-A1 and UI-R-E1 library
                     in the form of single-stranded circles. The remaining
                     single-stranded circles (subtracted library) was purified
                     by hydroxyapatite column chromatography, converted to
                     double-stranded circles and electroporated into DH10B
                     bacteria (Life Technologies) to generate the UI-R-C0
                     library. This procedure has been previously described
                     (Bonaldo, Lennon and Soares, Genome Research 6: 791-806,
                     1996)"
ORIGIN      
        1 cacgaggggg cactgaatta gggcaaatct taagtagttt tggagttgga ttttagtcct
       61 acaggtgagt tatgggtttg agcagggctt gctcctcacc cctgtgggga acacccacca
      121 gcccaaggat ccatgcacag ccctctcccc gccctaagga ccaccgcact caagacgtgt
      181 ggctgtagta agtcggtcag tcatcacgtc aggaccttac caccaacggg tcacttgttt
      241 cccggagttg agttttagtc tgcatgcagc aggtgggata gagctgtgca gagtctgtga
      301 cgtggctgag accctcgagt tcacactttg cacttctgcc tccttgcgtt gttttagagc
      361 cctggctccc ccaggaggta ctgagccgga gccgacttgg tgtgcagtgc agtgtcggca
      421 gttgtattga ccagaattgc aggccaggga ggctgtgctg gaatttgagc ctaactagtt
      481 aggtttttac aggaaaactt gttctaactt gggcgaccct caccagctgt ctccaagtgt
      541 atactgtggg ccta
//
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