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UI-R-BS2-bdo-a-11-0-UI.s1 UI-R-BS2 Rattus norvegicus cDNA clone UI-R-BS2-bdo-a-11-0-UI 3', mRNA sequence

GenBank: BF389726.1

FASTA Graphics 

LOCUS       BF389726                 634 bp    mRNA    linear   EST 27-JAN-2011
DEFINITION  UI-R-BS2-bdo-a-11-0-UI.s1 UI-R-BS2 Rattus norvegicus cDNA clone
            UI-R-BS2-bdo-a-11-0-UI 3', mRNA sequence.
ACCESSION   BF389726
VERSION     BF389726.1
DBLINK      BioSample: SAMN00161734
KEYWORDS    EST.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 634)
  AUTHORS   Bonaldo,M.F., Lennon,G. and Soares,M.B.
  TITLE     Normalization and subtraction: two approaches to facilitate gene
            discovery
  JOURNAL   Genome Res. 6 (9), 791-806 (1996)
   PUBMED   8889548
COMMENT     Contact: Soares, MB
            Cancer Biology & Epigenomics Program
            Children's Memorial Research Center
            2300 Children's Plaza, Box 220, Chicago, IL 60614-3394, USA
            Tel: 773 755 6551
            Fax: 773 755 6378
            Email: mbsoares@childrensmemorial.org
            The sequence contained an oligo-dT track that was present in the
            oligonucleotide that was used to prime the synthesis of first
            strand cDNA and therefore this may represent a bonafide poly A
            tail. The sequence tag present in the cDNA between the NotI site
            and the oligo-dT track served to verify it as a clone from the
            normalized embryo at 13 dpc library cDNA Library Preparation: M.B.
            Soares Lab Clone distribution: clones will be available through
            Research Genetics (www.resgen.com)
            Seq primer: M13 Forward
            POLYA=Yes.
FEATURES             Location/Qualifiers
     source          1..634
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="Sprague-Dawley"
                     /db_xref="taxon:10116"
                     /clone="UI-R-BS2-bdo-a-11-0-UI"
                     /clone_lib="SAMN00161734 UI-R-BS2"
                     /dev_stage="embryonic 13 dpc"
                     /lab_host="DH10B (Life Technologies)"
                     /note="Vector: pT7T3D-PacI; Site_1: Not I; Site_2: Eco RI;
                     The UI-R-BS2 library is a subtracted library derived from
                     13 dpc whole embryo tissue. For a detailed description of
                     the library from which this clone was derived, please
                     visit our web site at ratest.eng.uiowa.edu. The
                     subtraction has been previously described in (Bonaldo,
                     Lennon and Soares, Genome Research 6:791-806, 1996);
                     TAG_LIB=UI-R-BS2;
                     TAG_SEQ=AATCC;
                     TAG_TISSUE=embryo at 13 dpc"
ORIGIN      
        1 tttttttttt tttttttcaa gacacattat aatagctctt tatttcatga cccagcaatc
       61 tccaccccac ttaaagcact tccaggataa gcacaaacgg tccccaaaac tgctgtcatg
      121 attcaccagg cttaccctca ccctttatca caaaggcaca gaagtcacct gagacctcag
      181 tgtcagcgtg actgcacagc acactgatgt gcagcccaaa aaatattaaa agcatagagt
      241 tgcttcccaa gacttgtaga tttttgctat aattttagac taggccaata ttggcccgac
      301 aaggaatccg aacttctgca aaggctgtgt catattagcc tttaaagcag aaacatgtat
      361 ccactctgca aaagcgactt ctgcaaaggc tgtgtcatat tagcctttaa agcaggaaca
      421 tgaatccatt ctgcaaaagc gacttccaaa tgctagaaaa tatagctctc cctagactag
      481 ccacccactc atctccaggt cccagctaag gacattctag gtttgatctc caacaaaagg
      541 atggtaccaa ttcagtgtgt gggacatctt caccgggatg gctcagctgc acctgggtca
      601 catccaggaa aagagtctgg gttacagggg gtct
//
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