LOCUS AY046042 1433 bp mRNA linear PLN 18-SEP-2002
DEFINITION Arabidopsis thaliana putative sterol-C-methyltransferase
(At1g20330) mRNA, complete cds.
ACCESSION AY046042
VERSION AY046042.1
KEYWORDS FLI_CDNA.
SOURCE Arabidopsis thaliana (thale cress)
ORGANISM Arabidopsis thaliana
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae;
Pentapetalae; rosids; malvids; Brassicales; Brassicaceae;
Camelineae; Arabidopsis.
REFERENCE 1 (bases 1 to 1433)
AUTHORS Yamada,K., Liu,S.X., Sakano,H., Pham,P.K., Banh,J., Chung,M.K.,
Goldsmith,A.D., Lee,J.M., Quach,H.L., Toriumi,M., Yu,G., Bowser,L.,
Carninci,P., Chen,H., Cheuk,R., Hayashizaki,Y., Ishida,J.,
Jones,T., Kamiya,A., Karlin-Neumann,G., Kawai,J., Kim,C., Lam,B.,
Lin,J., Miranda,M., Narusaka,M., Nguyen,M., Palm,C.J., Sakurai,T.,
Satou,M., Seki,M., Shinn,P., Southwick,A., Shinozaki,K.,
Davis,R.W., Ecker,J.R. and Theologis,A.
TITLE Arabidopsis Full Length cDNA Clones
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 1433)
AUTHORS Yamada,K., Liu,S.X., Pham,P.K., Banh,J., Dale,J.M., Goldsmith,A.D.,
Jiang,P.X., Lee,J.M., Onodera,C.S., Quach,H.L., Tang,C.C.,
Toriumi,M., Yamamura,Y., Yu,G., Yu,S., Bowser,L., Carninci,P.,
Chen,H., Cheuk,R., Hayashizaki,Y., Ishida,J., Jones,T., Kamiya,A.,
Karlin-Neumann,G., Kawai,J., Kim,C., Koesema,E., Lam,B., Lin,J.,
Meyers,M.C., Miranda,M., Narusaka,M., Nguyen,M., Palm,C.J.,
Sakurai,T., Satou,M., Seki,M., Shinn,P., Southwick,A., Tracy,S.E.,
Shinozaki,K., Davis,R.W., Ecker,J.R. and Theologis,A.
TITLE Direct Submission
JOURNAL Submitted (09-JUL-2001) Plant Gene Expression Center, 800 Buchanan
Street, Albany, CA 94710, USA
COMMENT RIKEN Genomic Sciences Center (GSC) members carried out the
collection and clustering of RAFL cDNAs (RAFL cDNA : 'RIKEN
Arabidopsis Full-Length cDNA'): Seki,M., Narusaka,M., Ishida,J.,
Satou,M., Kamiya,A., Sakurai,T., Carninci,P., Kawai,J.,
Hayashizaki,Y. and Shinozaki,K.
The Salk, Stanford, PGEC (SSP) Consortium members carried out the
sequencing and annotation of the RAFL cDNAs: Yamada,K., Liu,S.X.,
Pham,P.K., Banh,J., Dale,J.M., Goldsmith,A.D., Jiang,P.X.,
Lee,J.M., Onodera,C.S., Quach,H.L., Tang,C.C., Toriumi,M.,
Yamamura,Y., Yu,G., Yu,S., Bowser,L., Chen,H., Cheuk,R., Jones,T.,
Karlin-Neumann,G., Kim,C., Koesema,E., Lam,B., Lin,J., Meyers,M.C.,
Miranda,M., Nguyen,M., Palm,C.J., Shinn,P., Southwick,A.,
Tracy,S.E., Davis,R.W., Ecker,J.R. and Theologis,A.
Yamada,K. (SSP/PGEC) and Seki,M. (RIKEN GSC) contributed equally to
this work. Shinozaki,K. (RIKEN GSC) and Theologis,A. (SSP/PGEC)
contributed equally to this work as PIs.
Annotation is based on the January 2002 version of the Arabidopsis
genome submitted to GenBank.
FEATURES Location/Qualifiers
source 1..1433
/organism="Arabidopsis thaliana"
/mol_type="mRNA"
/db_xref="taxon:3702"
/chromosome="1"
/clone="RAFL07-11-E12 (R10887)"
/ecotype="Columbia"
/note="This clone is in a modified pBluescript vector
(FLC-1) as a BamHI/XhoI insert."
gene 1..1433
/gene="At1g20330"
5'UTR 1..71
/gene="At1g20330"
misc_difference 2
/gene="At1g20330"
/note="compared to genomic sequence"
/replace="c"
CDS 72..1157
/gene="At1g20330"
/experiment="experimental evidence, no additional details
recorded"
/note="Member of the ubiE/COQ5 methyltransferase family
PF|01209"
/codon_start=1
/product="putative sterol-C-methyltransferase"
/protein_id="AAK76716.1"
/translation="MDSLTLFFTGALVAVGIYWFLCVLGPAERKGKRAVDLSGGSISA
EKVQDNYKQYWSFFRRPKEIETAEKVPDFVDTFYNLVTDIYEWGWGQSFHFSPSIPGK
SHKDATRLHEEMAVDLIQVKPGQKILDVGCGVGGPMRAIASHSRANVVGITINEYQVN
RARLHNKKAGLDALCEVVCGNFLQMPFDDNSFDGAYSIEATCHAPKLEEVYAEIYRVL
KPGSMYVSYEWVTTEKFKAEDDEHVEVIQGIERGDALPGLRAYVDIAETAKKVGFEIV
KEKDLASPPAEPWWTRLKMGRLAYWRNHIVVQILSAVGVAPKGTVDVHEMLFKTADYL
TRGGETGIFSPMHMILCRKPESPEESS"
3'UTR 1158..1433
/gene="At1g20330"
misc_difference 1350
/gene="At1g20330"
/note="compared to genomic sequence"
/replace="g"
misc_feature 1417
/gene="At1g20330"
/note="artifact within poly A tail"
ORIGIN
1 tgaggcaaag aagtaaaaga gacttaaaac tctctctctc tctctcttgg tcttcctcac
61 tcttaacgaa aatggactct ttaacactct tcttcaccgg tgcactcgtc gccgtcggta
121 tctactggtt cctctgcgtt ctcggtccag cagagcgtaa aggcaaacga gccgtagatc
181 tctctggtgg ctcaatctcc gccgagaaag tccaagacaa ctacaaacag tactggtctt
241 tcttccgccg tccaaaagaa atcgaaaccg ccgagaaagt tccagacttc gtcgacacat
301 tctacaatct cgtcaccgac atatacgagt ggggatgggg acaatccttc cacttctcac
361 catcaatccc cggaaaatct cacaaagacg ccacgcgcct ccacgaagag atggccgtag
421 atctgatcca agtcaaacct ggtcaaaaga tcctagacgt cggatgcggt gtcggcggtc
481 cgatgcgagc gattgcatct cactcgcgag ctaacgtagt cgggattaca ataaacgagt
541 atcaggtgaa cagagctcgt ctccacaata agaaagctgg tctcgacgcg ctttgcgagg
601 tcgtgtgtgg taacttcctc cagatgccgt tcgatgacaa cagtttcgac ggtgcttatt
661 ccatcgaagc cacgtgtcac gcgccgaagc tggaagaagt gtacgcagag atctacaggg
721 tgttgaaacc cggatctatg tatgtgtcgt acgagtgggt tacgacggag aaatttaagg
781 cggaggatga cgaacacgtg gaggtaatcc aagggattga gagaggcgat gcgttaccag
841 ggcttagggc ttacgtggat atagctgaga cggctaaaaa ggttgggttt gagatagtga
901 aggagaagga tctggcgagt ccaccggctg agccgtggtg gactaggctt aagatgggta
961 ggcttgctta ttggaggaat cacattgtgg ttcagatttt gtcagcggtt ggagttgctc
1021 ctaaaggaac tgttgatgtt catgagatgt tgtttaagac tgctgattat ttgaccagag
1081 gaggtgaaac cggaatattc tctccgatgc atatgattct ctgcagaaaa ccggagtcac
1141 cggaggagag ttcttgagaa aggtagaaag gaaacatcac cggaaaaagt atggagaatt
1201 ttctcaattt gtttttattt ttaagttaaa tcaacttggt tattgtacta tttttgtgtt
1261 ttaatttggt ttgtgtttca agaattatta gttttttttt gttttgttgc atatgagaat
1321 cttactcttg atttctccgc cgtagtgcca gcgagacata ggggattatt agtattttta
1381 agtgtgttta agattgatta acaagttagt aaaaaagaaa aaaaaaaaaa aaa
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