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L0017H10-3 NIA Mouse E12.5 Female Mesonephros and Gonads cDNA Library Mus musculus cDNA clone L0017H10 3', mRNA sequence

GenBank: AW547148.2

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LOCUS       AW547148                 567 bp    mRNA    linear   EST 12-MAY-2010
DEFINITION  L0017H10-3 NIA Mouse E12.5 Female Mesonephros and Gonads cDNA
            Library Mus musculus cDNA clone L0017H10 3', mRNA sequence.
ACCESSION   AW547148
VERSION     AW547148.2
DBLINK      BioSample: SAMN00158333
KEYWORDS    EST.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 567)
  AUTHORS   Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
            Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
            Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
  TITLE     Genome-wide expression profiling of mid-gestation placenta and
            embryo using a 15,000 mouse developmental cDNA microarray
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
   PUBMED   10922068
COMMENT     On Mar 7, 2000 this sequence version replaced AW547148.1.
            Contact: George J. Kargul
            Laboratory of Genetics
            National Institute on Aging/National Institutes of Health
            333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
            Email: cdna@lgsun.grc.nia.nih.gov
            Plate: L0017  row: H  column: 10
            Seq primer: -21M13 Forward
            POLYA=Yes.
FEATURES             Location/Qualifiers
     source          1..567
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6J"
                     /db_xref="niaEST:L0017H10-3"
                     /db_xref="taxon:10090"
                     /clone="L0017H10"
                     /sex="female"
                     /clone_lib="SAMN00158333 NIA Mouse E12.5 Female
                     Mesonephros and Gonads cDNA Library"
                     /dev_stage="12.5dpc"
                     /lab_host="DH10B"
                     /note="Vector: pSPORT1 (Gibco/BRL Life Technology);
                     Site_1: SalI; Site_2: NotI; Total RNAs were extracted from
                     2 Mesonephros. The double-stranded cDNA was synthesized by
                     Gibco's kit with an Oligo(dT) primer [NotI primer-adapter
                     from GibcoBRL]
                     [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'] from
                     3.42ug of total RNA . The double-stranded cDNAs were
                     treated with T4 DNA polymerase and purified by
                     ethanol-precipitation. The cDNAs were ligated to
                     Lone-linker LL-Sal3 (include Sal1 sequence). The cDNAs
                     were purified by phenol/chloroform and separated from free
                     linkers by Centricon 100. Then, cDNAs were amplified by
                     long-range high fidelity PCR using Takara's Ex Taq
                     polymerase. Then, the cDNAs were purified by
                     phenol/chloroform and by Centricon 100. The cDNAs were
                     digested with SalI and NotI enzymes. Then, the cDNAs were
                     size selected by Gibco's Size Fractionation Column. The
                     cDNAs were cloned into SalI/NotI site of pSPORT1 plasmid
                     vector. The DH10B E. coli host was transformed with the
                     ligation mixture by chemical method. The library was
                     constructed by Xiaohong Wang."
ORIGIN      
        1 acatagtcaa aatgctttta ttgttctgct gaaatgctta caaatactgc aaaacaccca
       61 accaggccca gcaactaagg gcccagtgct ggggagggca gggaaggtgg cttagtgtta
      121 aggcgcaagg ctgaggccag ccagctggag acttatcctc cgttctcctt tcccatcacc
      181 tttgggaaac tgaagggaga ttaccacagc tcagggcctc tgcttctgcc cgccccagcc
      241 ccaactcagg cttctttgca ggcactctga gctacccttg ttcctcactg ggaactagtc
      301 ctgttagaat gggagggcag acctcccggc ttcctctccc ctcagtgatg ctgtccctct
      361 ggtcttggat cccaggacat catcccttct ggctctaagc aaggcacagt aaaagggaga
      421 ggggctgggc tgggggctgg agacacactg cagccgggag gaattccatc tcctcccacc
      481 atgtgacatg cccccaggcc tgggttggag agatgatggc acgtaccctc atgtggccca
      541 cagccagaag cagtgggcaa agcacag
//
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