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GenBank: AW547148.2
FASTA Graphics
LOCUS AW547148 567 bp mRNA linear EST 12-MAY-2010 DEFINITION L0017H10-3 NIA Mouse E12.5 Female Mesonephros and Gonads cDNA Library Mus musculus cDNA clone L0017H10 3', mRNA sequence. ACCESSION AW547148 VERSION AW547148.2 DBLINK BioSample: SAMN00158333 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 567) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Mar 7, 2000 this sequence version replaced AW547148.1. Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov Plate: L0017 row: H column: 10 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..567 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:L0017H10-3" /db_xref="taxon:10090" /clone="L0017H10" /sex="female" /clone_lib="SAMN00158333 NIA Mouse E12.5 Female Mesonephros and Gonads cDNA Library" /dev_stage="12.5dpc" /lab_host="DH10B" /note="Vector: pSPORT1 (Gibco/BRL Life Technology); Site_1: SalI; Site_2: NotI; Total RNAs were extracted from 2 Mesonephros. The double-stranded cDNA was synthesized by Gibco's kit with an Oligo(dT) primer [NotI primer-adapter from GibcoBRL] [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'] from 3.42ug of total RNA . The double-stranded cDNAs were treated with T4 DNA polymerase and purified by ethanol-precipitation. The cDNAs were ligated to Lone-linker LL-Sal3 (include Sal1 sequence). The cDNAs were purified by phenol/chloroform and separated from free linkers by Centricon 100. Then, cDNAs were amplified by long-range high fidelity PCR using Takara's Ex Taq polymerase. Then, the cDNAs were purified by phenol/chloroform and by Centricon 100. The cDNAs were digested with SalI and NotI enzymes. Then, the cDNAs were size selected by Gibco's Size Fractionation Column. The cDNAs were cloned into SalI/NotI site of pSPORT1 plasmid vector. The DH10B E. coli host was transformed with the ligation mixture by chemical method. The library was constructed by Xiaohong Wang." ORIGIN 1 acatagtcaa aatgctttta ttgttctgct gaaatgctta caaatactgc aaaacaccca 61 accaggccca gcaactaagg gcccagtgct ggggagggca gggaaggtgg cttagtgtta 121 aggcgcaagg ctgaggccag ccagctggag acttatcctc cgttctcctt tcccatcacc 181 tttgggaaac tgaagggaga ttaccacagc tcagggcctc tgcttctgcc cgccccagcc 241 ccaactcagg cttctttgca ggcactctga gctacccttg ttcctcactg ggaactagtc 301 ctgttagaat gggagggcag acctcccggc ttcctctccc ctcagtgatg ctgtccctct 361 ggtcttggat cccaggacat catcccttct ggctctaagc aaggcacagt aaaagggaga 421 ggggctgggc tgggggctgg agacacactg cagccgggag gaattccatc tcctcccacc 481 atgtgacatg cccccaggcc tgggttggag agatgatggc acgtaccctc atgtggccca 541 cagccagaag cagtgggcaa agcacag //
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