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GenBank: AW291821.1
FASTA Graphics
LOCUS AW291821 411 bp mRNA linear EST 08-JAN-2011 DEFINITION UI-H-BI2-agl-e-02-0-UI.s1 NCI_CGAP_Sub4 Homo sapiens cDNA clone IMAGE:2724579 3', mRNA sequence. ACCESSION AW291821 VERSION AW291821.1 DBLINK BioSample: SAMN00157280 KEYWORDS EST. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 411) CONSRTM NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap TITLE National Cancer Institute, Cancer Genome Anatomy Project (CGAP), Tumor Gene Index JOURNAL Unpublished COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov The sequence contained an oligo-dT track that was present in the oligonucleotide that was used to prime the synthesis of first strand cDNA and therefore this may represent a bonafide poly A tail. cDNA Library Preparation: M.B. Soares Lab Clone distribution: NCI-CGAP clone distribution information can be found through the I.M.A.G.E. Consortium/LLNL at: www-bio.llnl.gov/bbrp/image/image.html Seq primer: M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..411 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="IMAGE:2724579" /clone_lib="SAMN00157280 NCI_CGAP_Sub4" /lab_host="DH10B (Life Technologies)" /note="Vector: pT7T3D-PacI; Site_1: Not I; Site_2: Eco RI; The NCI_CGAP_Sub4 library is a subtracted library derived from the NCI_CGAP_Sub2 library which is a subtracted library derived from the NCI_CGAP_Sub1 library, which is a subtracted library derived from BI. BI constitutes a mixture of 21 normalized or subtracted NCI_CGAP libraries: NCI_CGAP_Co4, NCI_CGAP_Pr22, NCI_CGAP_Pr28, NCI_CGAP_Co10, NCI_CGAP_Co16, NCI_CGAP_Kid5, NCI_CGAP_Kid12, NCI_CGAP_Kid3, NCI_CGAP_Kid11, NCI_CGAP_Lym2, NCI_CGAP_Br2, NCI_CGAP_Co8, NCI_CGAP_CLL1, NCI_CGAP_Lei2, NCI_CGAP_Brn23, NCI_CGAP_Lu5, NCI_CGAP_Lu24, NCI_CGAP_Lu19, NCI_CGAP_GC4, NCI_CGAP_GC6, NCI_CGAP_Brn25. These 21 libraries were pooled and a single-stranded DNA preparation of the resulting mixture was used as a tracer in a subtractive hybridization with a driver whose composition is detailed below: NCI_CGAP_Kid3 pool 1 : LLAM 3334-3337, 3682-3683, 3798-3803 (IMAGE CloneIDs 1322376-1323911, 1456008-1456775, 1500552-1502855) NCI_CGAP_Kid5 pool 1 : LLAM 3338-3342, 3722-3725, 3776-3778 (IMAGE CloneIDs 1323912-1325831, 1471368-1472903, 1492104-1493255) NCI_CGAP_Lu5 pool 1 : LLAM 3575-3582, 3851-3854 (IMAGE CloneIDs 1414920-1417991, 1520904-1522439) NCI_CGAP_GC4 pool 1 : LLAM 3164-3167, 3716-3720, 3733-3735 (IMAGE CloneIDs 1257096-1258631, 1469064-1470983, 1475592-1476743) NCI_CGAP_Pr22 pool 1 : LLAM 2457-2459, 2758-2759, 3062-3068 (IMAGE CloneIDs 985608-986759, 1101192-1101959, 1217928-1220615) NCI_CGAP_Co10 pool 1 : LLAM 2644-2653, 2871-2872 (IMAGE CloneIDs 1057416-1061255, 1144584-1145351) Subtraction was performed as previously described [Bonaldo, Lennon & Soares (1996): Normalization and Subtraction: Two Approaches To Facilitate Gene Discovery. Genome Research 6, 791-806.]; TAG_LIB=NCI_CGAP_Lu5; TAG_SEQ=CAAC; TAG_TISSUE=lung" ORIGIN 1 tttttttttt tttttttgat ctgaaaatga ctttaattgt ttaggctctg ggaagagatg 61 aaggataaaa taacatctac tttttacaaa acaatttata aaaatacaag cagaaatgaa 121 gccagagcag gtgacaaaca acctgcacac ctggaggatt ccaggcatct tcccaggaac 181 aggtgacccc tcacacattt gctatatctt ccagcagatg gttagcttgt gtcattatgc 241 cacactcctc tctgatggac acttctttgc cgtactccaa tctgggacta gaaagtcaag 301 gtgggaggaa gggataaggg gaagcaactt cagcccctct tcatctaagg ttctccccac 361 accatccaaa gcccctccag tgctgtctct ctccatctgt gttctccttt g //
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