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wz99g02.x1 Soares_NFL_T_GBC_S1 Homo sapiens cDNA clone IMAGE:2566994 3', mRNA sequence

GenBank: AW072308.1

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LOCUS       AW072308                 371 bp    mRNA    linear   EST 06-JAN-2011
DEFINITION  wz99g02.x1 Soares_NFL_T_GBC_S1 Homo sapiens cDNA clone
            IMAGE:2566994 3', mRNA sequence.
ACCESSION   AW072308
VERSION     AW072308.1
DBLINK      BioSample: SAMN00155629
KEYWORDS    EST.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 371)
  CONSRTM   NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap
  TITLE     National Cancer Institute, Cancer Genome Anatomy Project (CGAP),
            Tumor Gene Index
  JOURNAL   Unpublished
COMMENT     Contact: Robert Strausberg, Ph.D.
            Email: cgapbs-r@mail.nih.gov
            This clone is available royalty-free through LLNL ; contact the
            IMAGE Consortium (info@image.llnl.gov) for further information.
            Insert Length: 784   Std Error: 0.00
            Seq primer: -40UP from Gibco
            High quality sequence stop: 355.
FEATURES             Location/Qualifiers
     source          1..371
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /clone="IMAGE:2566994"
                     /clone_lib="SAMN00155629 Soares_NFL_T_GBC_S1"
                     /lab_host="DH10B"
                     /note="Organ: pooled; Vector: pT7T3D-PacI; Site_1: Not I;
                     Site_2: Eco RI; Equal amounts of plasmid DNA from three
                     normalized libraries (fetal lung NbHL19W, testis NHT, and
                     B-cell NCI_CGAP_GCB1) were mixed, and ss circles were made
                     in vitro. Following HAP purification, this DNA was used as
                     tracer in a subtractive hybridization reaction. The driver
                     was PCR-amplified cDNAs from pools of 5,000 clones made
                     from the same 3 libraries. The pools consisted of
                     I.M.A.G.E. clones 297480-302087, 682632-687239,
                     726408-728711, and 729096-731399. Subtraction by Bento
                     Soares and M. Fatima Bonaldo."
ORIGIN      
        1 tttgacacaa tacgcttctc atttatttta tatttataca aacagcgctt ccctgaaaaa
       61 tcagggtatc gttcaagatt ttatctttat tcacagaagt tttaaaattt ttaactttta
      121 tatgcacaag acagttctga tttgttgaat taagtgaccg cgggtgtgct aatgcttcta
      181 aaaaacagca ttagccttct cccatcaaaa gtccggaagc tgcccttcag tcgtcaaagt
      241 gtttgcctta atttgcaatc gttatgactt gagccaaatg cttatacctc atttgtgtcg
      301 tatatgtgaa gatacaattg caaatcgttc acgacctntg agtcaagact ttgagtttcc
      361 ttgaggtcag g
//
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