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bs08c04.y1 Drosophila melanogaster adult testis library Drosophila melanogaster cDNA clone bs08c04 5', mRNA sequence

GenBank: AI945036.2

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LOCUS       AI945036                 390 bp    mRNA    linear   EST 13-MAY-2010
DEFINITION  bs08c04.y1 Drosophila melanogaster adult testis library Drosophila
            melanogaster cDNA clone bs08c04 5', mRNA sequence.
ACCESSION   AI945036
VERSION     AI945036.2
DBLINK      BioSample: SAMN00156857
KEYWORDS    EST.
SOURCE      Drosophila melanogaster (fruit fly)
  ORGANISM  Drosophila melanogaster
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Diptera; Brachycera;
            Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora.
REFERENCE   1  (bases 1 to 390)
  AUTHORS   Andrews,J., Bouffard,G.G., Cheadle,C., Lu,J., Becker,K.G. and
            Oliver,B.
  TITLE     Gene discovery using computational and microarray analysis of
            transcription in the drosophila melanogaster testis
  JOURNAL   Genome Res. 10 (12), 2030-2043 (2000)
   PUBMED   11116097
COMMENT     On Aug 17, 1999 this sequence version replaced AI945036.1.
            Contact: Brian Oliver
            Laboratory of Cellular and Developmental Biology
            NIDDK, National Institutes of Health
            6 Center Drive MSC 2715, Bldg 6, Rm B1-13, Bethesda, MD 20892 USA
            Fax: (301) 496 5239
            Email: oliver@helix.nih.gov,
            http://www.niddk.nih.gov/intram/people/boliver.htm
            Tissue isolation and library construction performed at the National
            Institute of Diabetes and Digestive and Kidney Diseases, NIH (see
            http://www.niddk.nih.gov/intram/people/boliver.htm). DNA sequencing
            and analyses performed by National Institutes of Health Intramural
            Sequencing Center (NISC; see http://www.nisc.nih.gov).
            Plate: 08  row: c  column: 04
            Seq primer: M13RP1 reverse primer (ABI).
FEATURES             Location/Qualifiers
     source          1..390
                     /organism="Drosophila melanogaster"
                     /mol_type="mRNA"
                     /strain="y[*] w[67c1]/Y"
                     /db_xref="taxon:7227"
                     /clone="bs08c04"
                     /sex="male"
                     /clone_lib="SAMN00156857 Drosophila melanogaster adult
                     testis library"
                     /dev_stage="1-5 day adult"
                     /lab_host="SOLR (Stratagene)"
                     /note="Organ: testis; Vector: pBlueScript SK (Stratagene);
                     Site_1: EcoR I; Site_2: Xho I; Testes dissected from 1-5
                     day adult y[*] w[67c1]/Y males raised at 25oC. RNA
                     isolated using Trizol (Life Technologies) and a single
                     round of Poly(A)+ selection using Oligotex (Qiagen). cDNA
                     library constructed using Stratagene ZAP-cDNA syntesis
                     kit. Oligo dT-primed, size fractionated
                     1-6 kb, and directionally cloned at EcoRI and XhoI in
                     Uni-ZAP XR. Following a single round of amplification
                     pBlueScript SK phagemids were mass excised. A distribution
                     channel for clones is being sought, but not currently
                     available. Requests for clones cannot be honored."
ORIGIN      
        1 gcaccaggta gtattgtggt actatcctgt gccagtttta acttgcacaa gtcaaatcaa
       61 ttttgacgat agcgtcatcc aaaattgttt tgaaagttat cgtggtgtgg atttgcttaa
      121 agtaattgga gaatcaacta actcactaag gcctcctatt acgtttattc caacaatcac
      181 aatagatggc tcccaaggaa ggcaagaatc aatattaaaa gacttacttt ctgaagtgtg
      241 taaagcagct ggagactcca gagaagttaa agatatatgc aaaatttaaa aataaaaact
      301 gaaacttttg tttttaactt tctgtacttg ttctcaaaaa aaaaaatgtg aataaattta
      361 aaattttaag aataaaaaaa aaaaaaaaaa
//
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