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GenBank: AI945036.2
FASTA Graphics
LOCUS AI945036 390 bp mRNA linear EST 13-MAY-2010 DEFINITION bs08c04.y1 Drosophila melanogaster adult testis library Drosophila melanogaster cDNA clone bs08c04 5', mRNA sequence. ACCESSION AI945036 VERSION AI945036.2 DBLINK BioSample: SAMN00156857 KEYWORDS EST. SOURCE Drosophila melanogaster (fruit fly) ORGANISM Drosophila melanogaster Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora. REFERENCE 1 (bases 1 to 390) AUTHORS Andrews,J., Bouffard,G.G., Cheadle,C., Lu,J., Becker,K.G. and Oliver,B. TITLE Gene discovery using computational and microarray analysis of transcription in the drosophila melanogaster testis JOURNAL Genome Res. 10 (12), 2030-2043 (2000) PUBMED 11116097 COMMENT On Aug 17, 1999 this sequence version replaced AI945036.1. Contact: Brian Oliver Laboratory of Cellular and Developmental Biology NIDDK, National Institutes of Health 6 Center Drive MSC 2715, Bldg 6, Rm B1-13, Bethesda, MD 20892 USA Fax: (301) 496 5239 Email: oliver@helix.nih.gov, http://www.niddk.nih.gov/intram/people/boliver.htm Tissue isolation and library construction performed at the National Institute of Diabetes and Digestive and Kidney Diseases, NIH (see http://www.niddk.nih.gov/intram/people/boliver.htm). DNA sequencing and analyses performed by National Institutes of Health Intramural Sequencing Center (NISC; see http://www.nisc.nih.gov). Plate: 08 row: c column: 04 Seq primer: M13RP1 reverse primer (ABI). FEATURES Location/Qualifiers source 1..390 /organism="Drosophila melanogaster" /mol_type="mRNA" /strain="y[*] w[67c1]/Y" /db_xref="taxon:7227" /clone="bs08c04" /sex="male" /clone_lib="SAMN00156857 Drosophila melanogaster adult testis library" /dev_stage="1-5 day adult" /lab_host="SOLR (Stratagene)" /note="Organ: testis; Vector: pBlueScript SK (Stratagene); Site_1: EcoR I; Site_2: Xho I; Testes dissected from 1-5 day adult y[*] w[67c1]/Y males raised at 25oC. RNA isolated using Trizol (Life Technologies) and a single round of Poly(A)+ selection using Oligotex (Qiagen). cDNA library constructed using Stratagene ZAP-cDNA syntesis kit. Oligo dT-primed, size fractionated 1-6 kb, and directionally cloned at EcoRI and XhoI in Uni-ZAP XR. Following a single round of amplification pBlueScript SK phagemids were mass excised. A distribution channel for clones is being sought, but not currently available. Requests for clones cannot be honored." ORIGIN 1 gcaccaggta gtattgtggt actatcctgt gccagtttta acttgcacaa gtcaaatcaa 61 ttttgacgat agcgtcatcc aaaattgttt tgaaagttat cgtggtgtgg atttgcttaa 121 agtaattgga gaatcaacta actcactaag gcctcctatt acgtttattc caacaatcac 181 aatagatggc tcccaaggaa ggcaagaatc aatattaaaa gacttacttt ctgaagtgtg 241 taaagcagct ggagactcca gagaagttaa agatatatgc aaaatttaaa aataaaaact 301 gaaacttttg tttttaactt tctgtacttg ttctcaaaaa aaaaaatgtg aataaattta 361 aaattttaag aataaaaaaa aaaaaaaaaa //
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