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GenBank: AI849590.1
FASTA Graphics
LOCUS AI849590 288 bp mRNA linear EST 28-JAN-2011 DEFINITION UI-M-AH1-agr-e-05-0-UI.s1 NIH_BMAP_MCE_N Mus musculus cDNA clone UI-M-AH1-agr-e-05-0-UI 3', mRNA sequence. ACCESSION AI849590 VERSION AI849590.1 DBLINK BioSample: SAMN00156493 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 288) AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B. TITLE Normalization and subtraction: two approaches to facilitate gene discovery JOURNAL Genome Res. 6 (9), 791-806 (1996) PUBMED 8889548 COMMENT Contact: Chin, H National Institute of Mental Health 6001 Executive Blvd. Room 7N-7190, MSC 9643, Bethesda, MD 20892-9643, USA Tel: 301 443 1706 Fax: 301 443 9890 Email: mEST@mail.nih.gov The sequence contained an oligo-dT track that was present in the oligonucleotide that was used to prime the synthesis of first strand cDNA and therefore this may represent a bonafide poly A tail. The sequence tag present in the cDNA between the NotI site and the oligo-dT track served to verify it as a clone from the normalized cerebellum library cDNA Library Preparation: M.B. Soares Lab Clone distribution: NIH BMAP cDNA clones will be made available by the means that is soon to be determined. When NIH determines the means for distribution of the BMAP cDNA clones, this record will be updated accordingly when that means is determined. The following repetitive elements were found in this cDNA sequence: 1-49, >AT_rich#Low_complexity Seq primer: M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..288 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="taxon:10090" /clone="UI-M-AH1-agr-e-05-0-UI" /clone_lib="SAMN00156493 NIH_BMAP_MCE_N" /dev_stage="27-32 days" /lab_host="DH10B (Life Technologies)" /note="Vector: pT7T3D-PacI; Site_1: Not I; Site_2: Eco RI; The NIH_BMAP_MCE library is a normalized library constructed from mouse cerebellum. The tag is a string of 5 nucleotides present between the Not I site and the oligo-dT track. The library was constructed as described by Bonaldo, Lennon and Soares, Genome Research 6: 791-806, 1996. Tissue provided by Ms. Annie Novakovich, Zivic-Miller Laboratories. TAG_LIB=NIH_BMAP_MCE_N; TAG_SEQ=AACCC; TAG_TISSUE=cerebellum" ORIGIN 1 tttttttttg tttttttgtt tttgttttta attattaaga aaaaaatatg ttacaaaaca 61 atatcatcca tcatatattt cttaatttcc atttttttta aaaattaagg taataaaaaa 121 gtcactttat aaaataatac aaacgaacat gatcagtggg ggctgggagg gactgtctgc 181 gctgactggc tcaggggtcc agtgcacgct ttgcttctgg ctccctcccc aagctgggct 241 gggggggcta gggaaacaga tacggggtgg gaagggtccc tggactca //
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