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UI-M-AH1-agr-e-05-0-UI.s1 NIH_BMAP_MCE_N Mus musculus cDNA clone UI-M-AH1-agr-e-05-0-UI 3', mRNA sequence

GenBank: AI849590.1

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LOCUS       AI849590                 288 bp    mRNA    linear   EST 28-JAN-2011
DEFINITION  UI-M-AH1-agr-e-05-0-UI.s1 NIH_BMAP_MCE_N Mus musculus cDNA clone
            UI-M-AH1-agr-e-05-0-UI 3', mRNA sequence.
ACCESSION   AI849590
VERSION     AI849590.1
DBLINK      BioSample: SAMN00156493
KEYWORDS    EST.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 288)
  AUTHORS   Bonaldo,M.F., Lennon,G. and Soares,M.B.
  TITLE     Normalization and subtraction: two approaches to facilitate gene
            discovery
  JOURNAL   Genome Res. 6 (9), 791-806 (1996)
   PUBMED   8889548
COMMENT     Contact: Chin, H
            National Institute of Mental Health
            6001 Executive Blvd. Room 7N-7190, MSC 9643, Bethesda, MD
            20892-9643, USA
            Tel: 301 443 1706
            Fax: 301 443 9890
            Email: mEST@mail.nih.gov
            The sequence contained an oligo-dT track that was present in the
            oligonucleotide that was used to prime the synthesis of first
            strand cDNA and therefore this may represent a bonafide poly A
            tail. The sequence tag present in the cDNA between the NotI site
            and the oligo-dT track served to verify it as a clone from the
            normalized cerebellum library cDNA Library Preparation: M.B. Soares
            Lab Clone distribution: NIH BMAP cDNA clones will be made available
            by the means that is soon to be determined. When NIH determines the
            means for distribution of the BMAP cDNA clones, this record will be
            updated accordingly when that means is determined. The following
            repetitive elements were found in this cDNA sequence: 1-49,
            >AT_rich#Low_complexity
            Seq primer: M13 Forward
            POLYA=Yes.
FEATURES             Location/Qualifiers
     source          1..288
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6J"
                     /db_xref="taxon:10090"
                     /clone="UI-M-AH1-agr-e-05-0-UI"
                     /clone_lib="SAMN00156493 NIH_BMAP_MCE_N"
                     /dev_stage="27-32 days"
                     /lab_host="DH10B (Life Technologies)"
                     /note="Vector: pT7T3D-PacI; Site_1: Not I; Site_2: Eco RI;
                     The NIH_BMAP_MCE library is a normalized library
                     constructed from mouse cerebellum. The tag is a string of
                     5 nucleotides present between the Not I site and the
                     oligo-dT track. The library was constructed as described
                     by Bonaldo, Lennon and Soares, Genome Research 6: 791-806,
                     1996. Tissue provided by Ms. Annie Novakovich,
                     Zivic-Miller Laboratories.
                     TAG_LIB=NIH_BMAP_MCE_N;
                     TAG_SEQ=AACCC;
                     TAG_TISSUE=cerebellum"
ORIGIN      
        1 tttttttttg tttttttgtt tttgttttta attattaaga aaaaaatatg ttacaaaaca
       61 atatcatcca tcatatattt cttaatttcc atttttttta aaaattaagg taataaaaaa
      121 gtcactttat aaaataatac aaacgaacat gatcagtggg ggctgggagg gactgtctgc
      181 gctgactggc tcaggggtcc agtgcacgct ttgcttctgg ctccctcccc aagctgggct
      241 gggggggcta gggaaacaga tacggggtgg gaagggtccc tggactca
//
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