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nc08h04.x5 NCI_CGAP_Pr1 Homo sapiens cDNA clone IMAGE:1007575, mRNA sequence

GenBank: AI732080.1

FASTA Graphics 

LOCUS       AI732080                 329 bp    mRNA    linear   EST 05-JAN-2011
DEFINITION  nc08h04.x5 NCI_CGAP_Pr1 Homo sapiens cDNA clone IMAGE:1007575, mRNA
            sequence.
ACCESSION   AI732080
VERSION     AI732080.1
DBLINK      BioSample: SAMN00155176
KEYWORDS    EST.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 329)
  CONSRTM   NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap
  TITLE     National Cancer Institute, Cancer Genome Anatomy Project (CGAP),
            Tumor Gene Index
  JOURNAL   Unpublished
COMMENT     Contact: Robert Strausberg, Ph.D.
            Email: cgapbs-r@mail.nih.gov
            Tissue Procurement: W. Marston Linehan, M.D., Rodrigo Chuaqui,
            M.D., Michael Emmert-Buck, M.D., Ph.D.
             cDNA Library Preparation: David B. Krizman, Ph.D.
             cDNA Library Arrayed by: Genome Systems Inc., Greg Lennon, Ph.D.
             DNA Sequencing by: Washington University Genome Sequencing Center
             Clone distribution: NCI-CGAP clone distribution information can be
            found through the I.M.A.G.E. Consortium/LLNL at:
            www-bio.llnl.gov/bbrp/image/image.html
            
            This read is a RESEQUENCE of a previously sequenced human clone
            Original clone citation: WashU-NCI EST Project
            This read has been verified (found to hit its original self in the
            correct orientation)
            Insert Length: 673   Std Error: 0.00
            Seq primer: -40UP from Gibco.
FEATURES             Location/Qualifiers
     source          1..329
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /clone="IMAGE:1007575"
                     /sex="male"
                     /clone_lib="SAMN00155176 NCI_CGAP_Pr1"
                     /dev_stage="45 years old"
                     /lab_host="DH10B"
                     /note="Vector: pAMP10; Site_1: Not1; Site_2: EcoRI; 1st
                     strand cDNA was primed with oligo(dT)17 on 50 ng of
                     DNAse-treated, total cellular RNA obtained from
                     5,000-10,000 microdissected, histologically normal
                     prostate epithelial cells. Double-stranded cDNA was
                     ligated to EcoRI adaptors, 5 cycles of PCR applied to the
                     cDNA with an adaptor-specific primer, and the resulting
                     PCR product subcloned into pAMP10 by the UDG-cloning
                     method (Life Technologies). Average insert size is 600 bp.
                     NOTE: Not directionally cloned. This library was
                     constructed by David Krizman."
ORIGIN      
        1 gcgtcgacat tctatcctgc cagtgcttta gaataacttg cattttaaag tacatttgca
       61 cattttacat ataatgctat atggttgtgt ttgggctttg tgtacagatg acttttgtta
      121 ttaagtggaa aaggcctgaa atatgctgtt ctatgttaag taaattgata taacctaaga
      181 aaagatcttg ttgccaattc agaaaccata accttaaata gcatgcctgg gtctcctctg
      241 tatctggttt aacatctgca ttgaatattg gtattttcca ataatctcca actagcttat
      301 ttattttgaa aaaaaaaaaa aagtcgacg
//
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