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GenBank: AI732080.1
FASTA Graphics
LOCUS AI732080 329 bp mRNA linear EST 05-JAN-2011 DEFINITION nc08h04.x5 NCI_CGAP_Pr1 Homo sapiens cDNA clone IMAGE:1007575, mRNA sequence. ACCESSION AI732080 VERSION AI732080.1 DBLINK BioSample: SAMN00155176 KEYWORDS EST. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 329) CONSRTM NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap TITLE National Cancer Institute, Cancer Genome Anatomy Project (CGAP), Tumor Gene Index JOURNAL Unpublished COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov Tissue Procurement: W. Marston Linehan, M.D., Rodrigo Chuaqui, M.D., Michael Emmert-Buck, M.D., Ph.D. cDNA Library Preparation: David B. Krizman, Ph.D. cDNA Library Arrayed by: Genome Systems Inc., Greg Lennon, Ph.D. DNA Sequencing by: Washington University Genome Sequencing Center Clone distribution: NCI-CGAP clone distribution information can be found through the I.M.A.G.E. Consortium/LLNL at: www-bio.llnl.gov/bbrp/image/image.html This read is a RESEQUENCE of a previously sequenced human clone Original clone citation: WashU-NCI EST Project This read has been verified (found to hit its original self in the correct orientation) Insert Length: 673 Std Error: 0.00 Seq primer: -40UP from Gibco. FEATURES Location/Qualifiers source 1..329 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="IMAGE:1007575" /sex="male" /clone_lib="SAMN00155176 NCI_CGAP_Pr1" /dev_stage="45 years old" /lab_host="DH10B" /note="Vector: pAMP10; Site_1: Not1; Site_2: EcoRI; 1st strand cDNA was primed with oligo(dT)17 on 50 ng of DNAse-treated, total cellular RNA obtained from 5,000-10,000 microdissected, histologically normal prostate epithelial cells. Double-stranded cDNA was ligated to EcoRI adaptors, 5 cycles of PCR applied to the cDNA with an adaptor-specific primer, and the resulting PCR product subcloned into pAMP10 by the UDG-cloning method (Life Technologies). Average insert size is 600 bp. NOTE: Not directionally cloned. This library was constructed by David Krizman." ORIGIN 1 gcgtcgacat tctatcctgc cagtgcttta gaataacttg cattttaaag tacatttgca 61 cattttacat ataatgctat atggttgtgt ttgggctttg tgtacagatg acttttgtta 121 ttaagtggaa aaggcctgaa atatgctgtt ctatgttaag taaattgata taacctaaga 181 aaagatcttg ttgccaattc agaaaccata accttaaata gcatgcctgg gtctcctctg 241 tatctggttt aacatctgca ttgaatattg gtattttcca ataatctcca actagcttat 301 ttattttgaa aaaaaaaaaa aagtcgacg //
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