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fb81d12.x1 Zebrafish WashU MPIMG EST Danio rerio cDNA clone IMAGE:3718295 3', mRNA sequence

GenBank: AI545450.1

FASTA Graphics 

LOCUS       AI545450                 433 bp    mRNA    linear   EST 07-JUN-2001
DEFINITION  fb81d12.x1 Zebrafish WashU MPIMG EST Danio rerio cDNA clone
            IMAGE:3718295 3', mRNA sequence.
ACCESSION   AI545450
VERSION     AI545450.1
DBLINK      BioSample: SAMN00156101
KEYWORDS    EST.
SOURCE      Danio rerio (zebrafish)
  ORGANISM  Danio rerio
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Ostariophysi;
            Cypriniformes; Danionidae; Danioninae; Danio.
REFERENCE   1  (bases 1 to 433)
  AUTHORS   Clark,M., Johnson,S.L., Lehrach,H., Lee,R., Li,F., Marra,M.,
            Eddy,S., Hillier,L., Kucaba,T., Martin,J., Beck,C., Wylie,T.,
            Underwood,K., Steptoe,M., Theising,B., Allen,M., Bowers,Y.,
            Person,B., Swaller,T., Gibbons,M., Pape,D., Harvey,N., Schurk,R.,
            Ritter,E., Kohn,S., Shin,T., Jackson,Y., Cardenas,M., McCann,R.,
            Waterston,R. and Wilson,R.
  TITLE     WashU Zebrafish EST Project 1998
  JOURNAL   Unpublished
COMMENT     Contact: Stephen L. Johnson
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: zbrafish@watson.wustl.edu
            cDNA Library Preparation: Matthew Clark. cDNA Library Arrayed by:
            Matthew Clark. DNA Sequencing by: Washington University Genome
            Sequencing Center Clone distribution: Genome Systems, St. Louis,
            Missouri (web address: www.genomesystems.com) (email contact:
            info@genomesystems.com) and Research Genetics, Huntsville, Alabama
            (web address: www.resgen.com) (email contact: info@resgen.com) and
            RessourcenZentrumPrimarDatenbank, Berlin, Germany (web address:
            www.rzpd.de)
            Seq primer: T7 ET from Amersham
            High quality sequence stop: 404
            POLYA=No.
FEATURES             Location/Qualifiers
     source          1..433
                     /organism="Danio rerio"
                     /mol_type="mRNA"
                     /db_xref="taxon:7955"
                     /clone="IMAGE:3718295"
                     /sex="mixed"
                     /tissue_type="26 somite embryos, adult livers, shield
                     stage embryos"
                     /clone_lib="SAMN00156101 Zebrafish WashU MPIMG EST"
                     /lab_host="XL1-blue MRF"
                     /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1st
                     strand cDNA was primed with a Not I - oligo(dT)15 primer
                     [5'pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT3'];
                     double-stranded cDNA was ligated to Sal I adaptors (BRL),
                     digested with Not I and cloned into the Not I and Sal I
                     sites of the pSPORT1 vector (BRL). Library was constructed
                     by Matthew Clark (Lehrach lab; ICRF, London and Max Planck
                     Institut fuer Molekulare Genetik,Berlin). cDNAs for EST
                     analysis were selected following oligonucleotide
                     hybridization fingerprinting of arrayed clones from
                     zebrafish late somitogenesis (26 ss), adult liver or
                     embryonic shield stage (5.6 h) libraries. Fingerprint data
                     were used to computationally cluster cDNAs, and a single
                     cDNA from each cluster was chosen for sequencing. In some
                     cases multiple members of the same cluster were sequenced
                     to assess clustering parameters or single clones were
                     sequenced additional times to assess quality control."
ORIGIN      
        1 tgggtttttt tttttttttt ttggggcctt tacatatatt taatagggca ttttgaccac
       61 aattaatctg tacagaaata acattaataa taaaaaagat aaacaacttt tctcatttct
      121 gtaacttagg gtaacattta caataaaata atatacgtaa aaaaaaataa taacatgata
      181 taagggatat aaatacagag aggtttaaat taactggcat tggcatgcgt tattctctgt
      241 ttggcagaat aagtttaatt tacacacgat tatgatacac cgtcatagac aggcacataa
      301 gtagtaataa atcgagacat caaacccagt cggtcaattt tcagcaggag tcatctgaaa
      361 acccaaaaca aaaggaaatc tgttgagcaa tcatacaaac tcccaccctg atctgctctg
      421 tgaccgcagg cct
//
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