LOCUS AI545450 433 bp mRNA linear EST 07-JUN-2001
DEFINITION fb81d12.x1 Zebrafish WashU MPIMG EST Danio rerio cDNA clone
IMAGE:3718295 3', mRNA sequence.
ACCESSION AI545450
VERSION AI545450.1
DBLINK BioSample: SAMN00156101
KEYWORDS EST.
SOURCE Danio rerio (zebrafish)
ORGANISM Danio rerio
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Actinopterygii; Neopterygii; Teleostei; Ostariophysi;
Cypriniformes; Danionidae; Danioninae; Danio.
REFERENCE 1 (bases 1 to 433)
AUTHORS Clark,M., Johnson,S.L., Lehrach,H., Lee,R., Li,F., Marra,M.,
Eddy,S., Hillier,L., Kucaba,T., Martin,J., Beck,C., Wylie,T.,
Underwood,K., Steptoe,M., Theising,B., Allen,M., Bowers,Y.,
Person,B., Swaller,T., Gibbons,M., Pape,D., Harvey,N., Schurk,R.,
Ritter,E., Kohn,S., Shin,T., Jackson,Y., Cardenas,M., McCann,R.,
Waterston,R. and Wilson,R.
TITLE WashU Zebrafish EST Project 1998
JOURNAL Unpublished
COMMENT Contact: Stephen L. Johnson
Washington University School of Medicine
4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
Tel: 314 286 1800
Fax: 314 286 1810
Email: zbrafish@watson.wustl.edu
cDNA Library Preparation: Matthew Clark. cDNA Library Arrayed by:
Matthew Clark. DNA Sequencing by: Washington University Genome
Sequencing Center Clone distribution: Genome Systems, St. Louis,
Missouri (web address: www.genomesystems.com) (email contact:
info@genomesystems.com) and Research Genetics, Huntsville, Alabama
(web address: www.resgen.com) (email contact: info@resgen.com) and
RessourcenZentrumPrimarDatenbank, Berlin, Germany (web address:
www.rzpd.de)
Seq primer: T7 ET from Amersham
High quality sequence stop: 404
POLYA=No.
FEATURES Location/Qualifiers
source 1..433
/organism="Danio rerio"
/mol_type="mRNA"
/db_xref="taxon:7955"
/clone="IMAGE:3718295"
/sex="mixed"
/tissue_type="26 somite embryos, adult livers, shield
stage embryos"
/clone_lib="SAMN00156101 Zebrafish WashU MPIMG EST"
/lab_host="XL1-blue MRF"
/note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1st
strand cDNA was primed with a Not I - oligo(dT)15 primer
[5'pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT3'];
double-stranded cDNA was ligated to Sal I adaptors (BRL),
digested with Not I and cloned into the Not I and Sal I
sites of the pSPORT1 vector (BRL). Library was constructed
by Matthew Clark (Lehrach lab; ICRF, London and Max Planck
Institut fuer Molekulare Genetik,Berlin). cDNAs for EST
analysis were selected following oligonucleotide
hybridization fingerprinting of arrayed clones from
zebrafish late somitogenesis (26 ss), adult liver or
embryonic shield stage (5.6 h) libraries. Fingerprint data
were used to computationally cluster cDNAs, and a single
cDNA from each cluster was chosen for sequencing. In some
cases multiple members of the same cluster were sequenced
to assess clustering parameters or single clones were
sequenced additional times to assess quality control."
ORIGIN
1 tgggtttttt tttttttttt ttggggcctt tacatatatt taatagggca ttttgaccac
61 aattaatctg tacagaaata acattaataa taaaaaagat aaacaacttt tctcatttct
121 gtaacttagg gtaacattta caataaaata atatacgtaa aaaaaaataa taacatgata
181 taagggatat aaatacagag aggtttaaat taactggcat tggcatgcgt tattctctgt
241 ttggcagaat aagtttaatt tacacacgat tatgatacac cgtcatagac aggcacataa
301 gtagtaataa atcgagacat caaacccagt cggtcaattt tcagcaggag tcatctgaaa
361 acccaaaaca aaaggaaatc tgttgagcaa tcatacaaac tcccaccctg atctgctctg
421 tgaccgcagg cct
//