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GenBank: AI145598.1
FASTA Graphics
LOCUS AI145598 403 bp mRNA linear EST 26-JAN-2011 DEFINITION UI-R-BT0-ps-b-04-0-UI.s1 UI-R-BT0 Rattus norvegicus cDNA clone UI-R-BT0-ps-b-04-0-UI 3', mRNA sequence. ACCESSION AI145598 VERSION AI145598.1 DBLINK BioSample: SAMN00156004 KEYWORDS EST. SOURCE Rattus norvegicus (Norway rat) ORGANISM Rattus norvegicus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus. REFERENCE 1 (bases 1 to 403) AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B. TITLE Normalization and subtraction: two approaches to facilitate gene discovery JOURNAL Genome Res. 6 (9), 791-806 (1996) PUBMED 8889548 COMMENT Contact: Soares, MB Cancer Biology & Epigenomics Program Children's Memorial Research Center 2300 Children's Plaza, Box 220, Chicago, IL 60614-3394, USA Tel: 773 755 6551 Fax: 773 755 6378 Email: mbsoares@childrensmemorial.org The sequence tag present in the cDNA between the NotI site and the oligo-dT track served to identify it as a clone from the normalized adult NRBmedulla library. cDNA Library Preparation: M. Fatima Bonaldo, Ph.D. Clone distribution: clones will be available through Research Genetics This clone is also available through the I.M.A.G.E. Consortium at LLNL (info@image.llnl.gov). IMAGE ID=1788149 The following repetitive elements were found in this cDNA sequence: 1-94, >ID_RN#SINE/ID Seq primer: M13 Forward POLYA=No. FEATURES Location/Qualifiers source 1..403 /organism="Rattus norvegicus" /mol_type="mRNA" /strain="Sprague-Dawley" /db_xref="taxon:10116" /clone="UI-R-BT0-ps-b-04-0-UI" /clone_lib="SAMN00156004 UI-R-BT0" /dev_stage="adult" /lab_host="DH10B (Life Technologies)" /note="Vector: pT7T3D-PacI; Site_1: Not I; Site_2: Eco RI; This library (UI-R-BT0) consists of a mixture of individually tagged normalized libraries constructed from rat hippocampus, thalamus, mid-brain, medulla, corpus striatum, cerebral cortex and testis. The tag used to identify the source tissue is a string of 3-6 nucleotides present between the Not I site and the oligo-dT track which allows identification of the library of origin of a clone within the mixture. This library was then subtracted using a driver consisting of a mixture of all clones from UI-R-A0, UI-R-A1, UI-R-E0, UI-R-E1, UI-R-C0, UI-R-C1, UI-R-C2 and UI-R-C2p." ORIGIN 1 tttttggttc tttttttcgg agctggggac cgaacccagg gccttgcgct tcctaggtaa 61 gcgctctacc taccgctgag ctaaatcccc agcccccaga aatgccttta gtttctgccc 121 atttaggcaa aagtctggtg ggtgtggaag gtgggagagc aggcgagagc gcccactctt 181 ggcttctact ggttggcaca gtatcaggaa gctctgcaat gcaacctcag gactgacatg 241 ggaaggtgtg tacttactag ggtgatctga aggcactatg agaaccaacc attttctctc 301 ccagctacat tggtcctgtg acgcaagaaa ctcagaacag gaagttcaaa tccagtcagt 361 acccgagggc ctctttctca gtgtgaggcg ctgttgcacc tgc //
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