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GenBank: AI091296.1
FASTA Graphics
LOCUS AI091296 617 bp mRNA linear EST 06-JAN-2011 DEFINITION ow62d05.x1 Soares_NSF_F8_9W_OT_PA_P_S1 Homo sapiens cDNA clone IMAGE:1651401 3', mRNA sequence. ACCESSION AI091296 VERSION AI091296.1 DBLINK BioSample: SAMN00155770 KEYWORDS EST. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 617) CONSRTM NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap TITLE National Cancer Institute, Cancer Genome Anatomy Project (CGAP), Tumor Gene Index JOURNAL Unpublished COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov This clone is available royalty-free through LLNL ; contact the IMAGE Consortium (info@image.llnl.gov) for further information. Insert Length: 1233 Std Error: 0.00 Seq primer: -40m13 fwd. ET from Amersham High quality sequence stop: 456. FEATURES Location/Qualifiers source 1..617 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="IMAGE:1651401" /clone_lib="SAMN00155770 Soares_NSF_F8_9W_OT_PA_P_S1" /lab_host="DH10B" /note="Organ: pooled; Vector: pT7T3D-PacI; Site_1: Not I; Site_2: Eco RI; Equal amounts of plasmid DNA from five normalized libraries were mixed, and ss circles were made in vitro. Following HAP purification, this DNA was used as tracer in a subtractive hybridization reaction. The driver was PCR-amplified cDNAs from pools of 5,000 clones made from the same 5 libraries. The pools consisted of the following libraries and cloneIDs: Soares NbHSF pool 1: 309384-310919, 323208-325895 Soares Nb2HP pool 1: 145032-147335, 147720-148103, 148872-149255, 15002 - 150407, 151176-152327 Soares Nb2HF8-9W pool 1: 758280-760583, 772104-774407 Soares NbHPA pool 1: 304776-306311, 320136-322823, 326280-326663 Soares NbHOT pool 1: 723720-726407, 739080-740999 Subtraction by Bento Soares and M. Fatima Bonaldo." ORIGIN 1 tttttacaaa gtgatcttag gtttttcagt ttcatttatt tttaaagtat tttcacatat 61 ctttccattt ttcttccatg cttagatgac atcctaatga attacattaa taaattcaat 121 gtatggacat agcatttaca tgaaagcagt ttgtttaagt tctatttatt ttttatcccc 181 tccaactcca aagtaattga tcactaagtg cttaaaaaaa gagcttcttt actttgacag 241 aatatgtgca tgcctgcaca aaaatgcagg ctttttgatt ctaagcaaaa tgaaaattaa 301 agttacctaa agagttgctt tttccagatg cagaagggca cagaaaatac agtacttacc 361 cagaaagagt gaaaagagaa gtgttcagtc agggtttctg agtctcaaca cccaccatct 421 cccatccaga cccaaatcaa tcttaagata ttgctagaat ttgccatgca tttaaataga 481 acttcaaagg ggaaatgaga cgggatggca actctttnta cactgaagga atagaacaga 541 tagaattccc tgtagctggg tgaccgaatt ctcagccaga gtgcaatcgg caaaaggatt 601 acttggtggt ggagagc //
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