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ow62d05.x1 Soares_NSF_F8_9W_OT_PA_P_S1 Homo sapiens cDNA clone IMAGE:1651401 3', mRNA sequence

GenBank: AI091296.1

FASTA Graphics 

LOCUS       AI091296                 617 bp    mRNA    linear   EST 06-JAN-2011
DEFINITION  ow62d05.x1 Soares_NSF_F8_9W_OT_PA_P_S1 Homo sapiens cDNA clone
            IMAGE:1651401 3', mRNA sequence.
ACCESSION   AI091296
VERSION     AI091296.1
DBLINK      BioSample: SAMN00155770
KEYWORDS    EST.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 617)
  CONSRTM   NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap
  TITLE     National Cancer Institute, Cancer Genome Anatomy Project (CGAP),
            Tumor Gene Index
  JOURNAL   Unpublished
COMMENT     Contact: Robert Strausberg, Ph.D.
            Email: cgapbs-r@mail.nih.gov
            This clone is available royalty-free through LLNL ; contact the
            IMAGE Consortium (info@image.llnl.gov) for further information.
            Insert Length: 1233   Std Error: 0.00
            Seq primer: -40m13 fwd. ET from Amersham
            High quality sequence stop: 456.
FEATURES             Location/Qualifiers
     source          1..617
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /clone="IMAGE:1651401"
                     /clone_lib="SAMN00155770 Soares_NSF_F8_9W_OT_PA_P_S1"
                     /lab_host="DH10B"
                     /note="Organ: pooled; Vector: pT7T3D-PacI; Site_1: Not I;
                     Site_2: Eco RI; Equal amounts of plasmid DNA from five
                     normalized libraries were mixed, and ss circles were made
                     in vitro. Following HAP purification, this DNA was used as
                     tracer in a subtractive hybridization reaction. The driver
                     was PCR-amplified cDNAs from pools of 5,000 clones made
                     from the same 5 libraries. The pools consisted of the
                     following libraries and cloneIDs: Soares NbHSF pool 1:
                     309384-310919, 323208-325895 Soares Nb2HP pool 1:
                     145032-147335, 147720-148103, 148872-149255, 15002 -
                     150407, 151176-152327 Soares Nb2HF8-9W pool 1:
                     758280-760583, 772104-774407 Soares NbHPA pool 1:
                     304776-306311, 320136-322823, 326280-326663 Soares NbHOT
                     pool 1: 723720-726407, 739080-740999 Subtraction by Bento
                     Soares and M. Fatima Bonaldo."
ORIGIN      
        1 tttttacaaa gtgatcttag gtttttcagt ttcatttatt tttaaagtat tttcacatat
       61 ctttccattt ttcttccatg cttagatgac atcctaatga attacattaa taaattcaat
      121 gtatggacat agcatttaca tgaaagcagt ttgtttaagt tctatttatt ttttatcccc
      181 tccaactcca aagtaattga tcactaagtg cttaaaaaaa gagcttcttt actttgacag
      241 aatatgtgca tgcctgcaca aaaatgcagg ctttttgatt ctaagcaaaa tgaaaattaa
      301 agttacctaa agagttgctt tttccagatg cagaagggca cagaaaatac agtacttacc
      361 cagaaagagt gaaaagagaa gtgttcagtc agggtttctg agtctcaaca cccaccatct
      421 cccatccaga cccaaatcaa tcttaagata ttgctagaat ttgccatgca tttaaataga
      481 acttcaaagg ggaaatgaga cgggatggca actctttnta cactgaagga atagaacaga
      541 tagaattccc tgtagctggg tgaccgaatt ctcagccaga gtgcaatcgg caaaaggatt
      601 acttggtggt ggagagc
//
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