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GenBank: AI059986.1
FASTA Graphics
LOCUS AI059986 330 bp mRNA linear EST 26-JAN-2011 DEFINITION UI-R-C1-la-h-06-0-UI.s1 UI-R-C1 Rattus norvegicus cDNA clone UI-R-C1-la-h-06-0-UI 3', mRNA sequence. ACCESSION AI059986 VERSION AI059986.1 DBLINK BioSample: SAMN00155931 KEYWORDS EST. SOURCE Rattus norvegicus (Norway rat) ORGANISM Rattus norvegicus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus. REFERENCE 1 (bases 1 to 330) AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B. TITLE Normalization and subtraction: two approaches to facilitate gene discovery JOURNAL Genome Res. 6 (9), 791-806 (1996) PUBMED 8889548 COMMENT Contact: Soares, MB Cancer Biology & Epigenomics Program Children's Memorial Research Center 2300 Children's Plaza, Box 220, Chicago, IL 60614-3394, USA Tel: 773 755 6551 Fax: 773 755 6378 Email: mbsoares@childrensmemorial.org The sequence tag present in the cDNA between the NotI site and the oligo-dT track served to identify it as a clone from the normalized adult Lung library. cDNA Library Preparation: M. Fatima Bonaldo, Ph.D. Clone distribution: clones will be available through Research Genetics This clone is also available through the I.M.A.G.E. Consortium at LLNL (info@image.llnl.gov). IMAGE ID=1775753 Seq primer: M13 Forward POLYA=No. FEATURES Location/Qualifiers source 1..330 /organism="Rattus norvegicus" /mol_type="mRNA" /strain="Sprague-Dawley" /db_xref="taxon:10116" /clone="UI-R-C1-la-h-06-0-UI" /clone_lib="SAMN00155931 UI-R-C1" /dev_stage="Adult" /lab_host="DH10B (Life Technologies)" /note="Vector: pT7T3D-PacI; Site_1: Not I; Site_2: Eco RI; The UI-R-C1 library is a subtracted library derived from the UI-R-C0 library, which is a subtracted library derived from the UI-R-A1 and UI-R-E1 libraries. The UI-R-A1 library consisted of a mixture of individually tagged normalized libraries constructed from rat placenta, adult lung, brain, liver, kidney, heart, spleen, ovary, and muscle. The UI-R-E1 library consisted of a mixture of individually tagged normalized libraries constructed from 8, 12 and 18-day embryo. The tag is a string of 3-5 nucleotides present between the Not I site and the oligo-dT track which allows identification of the library of origin of a clone within the mixture. The subtracted library (UI-R-C1) was constructed as follows: PCR amplified cDNA inserts from UI-R-C0 clones from which 3' ESTs had been derived was used as a driver in a hybridization with the UI-R-C0 library in the form of single-stranded circles. The remaining single-stranded circles (subtracted library) was purified by hydroxyapatite column chromatography, converted to double-stranded circles and electroporated into DH10B bacteria (Life Technologies) to generate the UI-R-C1 library. This procedure has been previously described (Bonaldo, Lennon and Soares, Genome Research 6: 791-806, 1996)" ORIGIN 1 tttttttttt tttttttgag tggtacctta tttttatttt aacactgata atgttaataa 61 ttgtgatata atgtagtaac gaaatttaat acctcaactc cttttgttct ttctctttat 121 caagcctttt aaaatacata gttcaataca taaaatagtc aaaatgaatc ccaaaccact 181 tcaccttgcc aaacagaaac tacacatctg tgctgtccca gcacctggca tctactctct 241 ttctccctcc aggaactgaa atacatgtta ttggtatttt ttgtaggggg ctattttttt 301 ttaactgaaa tccacatatt aatcaagttt //
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