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GenBank: AA859710.1
FASTA Graphics
LOCUS AA859710 336 bp mRNA linear EST 26-JAN-2011 DEFINITION UI-R-E0-bx-g-09-0-UI.s1 UI-R-E0 Rattus norvegicus cDNA clone UI-R-E0-bx-g-09-0-UI 3', mRNA sequence. ACCESSION AA859710 VERSION AA859710.1 DBLINK BioSample: SAMN00155713 KEYWORDS EST. SOURCE Rattus norvegicus (Norway rat) ORGANISM Rattus norvegicus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus. REFERENCE 1 (bases 1 to 336) AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B. TITLE Normalization and subtraction: two approaches to facilitate gene discovery JOURNAL Genome Res. 6 (9), 791-806 (1996) PUBMED 8889548 COMMENT On Mar 10, 1998 this sequence version replaced gi:2949230. Contact: Soares, MB Cancer Biology & Epigenomics Program Children's Memorial Research Center 2300 Children's Plaza, Box 220, Chicago, IL 60614-3394, USA Tel: 773 755 6551 Fax: 773 755 6378 Email: mbsoares@childrensmemorial.org The sequence tag present in the cDNA between the NotI site and the oligo-dT track served to identify it as a clone from the normalized adult 18-Day-Embryo library. cDNA Library Preparation: M. Fatima Bonaldo, Ph.D. Clone distribution: clones will be available through Research Genetics This clone is also available through the I.M.A.G.E. Consortium at LLNL (info@image.llnl.gov). IMAGE ID=1769961 Seq primer: M13 Forward POLYA=No. FEATURES Location/Qualifiers source 1..336 /organism="Rattus norvegicus" /mol_type="mRNA" /strain="Sprague-Dawley" /db_xref="taxon:10116" /clone="UI-R-E0-bx-g-09-0-UI" /clone_lib="SAMN00155713 UI-R-E0" /dev_stage="embryonic" /lab_host="DH10B (Life Technologies)" /note="Vector: pT7T3D-PacI; Site_1: NotI; Site_2: EcoRI; This library consists of a mixture of individually tagged normalized libraries constructed from 8, 12 and 18-day embryo. The tag is a string of 3-5 nucleotides present between the Not I site and the oligo-dT track which allows identification of the library of origin of a clone within the mixture." ORIGIN 1 tttttttttt tttttttcag atttcaagat atttttattt taaacggttt tattataaaa 61 ctaatacaaa taacaaatga aatgtaaaga aggaaagcag tccacttgct gccctgccat 121 cctacaggga ccatcgctgg tgatctgatt gtcaactttc acagcctggt gtgtcattcg 181 catccatgcc acgttaacgc tgtggccctt cccttacggt agtgttgtcc ccatgctgct 241 cacggatacc tgcctatcga ctaaagctgt gggacagcag agacacagcc agcctccgcc 301 actctgaacg tccccttggg gtaaaccccc atgact //
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