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mi22a08.r1 Soares mouse embryo NbME13.5 14.5 Mus musculus cDNA clone IMAGE:464246 5', mRNA sequence

GenBank: AA032906.1

FASTA Graphics 

LOCUS       AA032906                 390 bp    mRNA    linear   EST 22-AUG-1996
DEFINITION  mi22a08.r1 Soares mouse embryo NbME13.5 14.5 Mus musculus cDNA
            clone IMAGE:464246 5', mRNA sequence.
ACCESSION   AA032906
VERSION     AA032906.1
DBLINK      BioSample: SAMN00155048
KEYWORDS    EST.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 390)
  AUTHORS   Marra,M., Hillier,L., Allen,M., Bowles,M., Dietrich,N., Dubuque,T.,
            Geisel,S., Kucaba,T., Lacy,M., Le,M., Martin,J., Morris,M.,
            Schellenberg,K., Steptoe,M., Tan,F., Underwood,K., Moore,B.,
            Theising,B., Wylie,T., Lennon,G., Soares,B., Wilson,R. and
            Waterston,R.
  TITLE     The WashU-HHMI Mouse EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Marra M/Mouse EST Project
            WashU-HHMI Mouse EST Project
            Washington University School of MedicineP
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: mouseest@watson.wustl.edu
            This clone is available royalty-free through LLNL ; contact the
            IMAGE Consortium (info@image.llnl.gov) for further information.
            MGI:278062
            Seq primer: -28M13 rev2 from Amersham.
FEATURES             Location/Qualifiers
     source          1..390
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6J"
                     /db_xref="taxon:10090"
                     /clone="IMAGE:464246"
                     /sex="unknown"
                     /tissue_type="embryo"
                     /clone_lib="SAMN00155048 Soares mouse embryo NbME13.5
                     14.5"
                     /dev_stage="13.5-14.5dpc total fetus"
                     /lab_host="DH10B"
                     /note="Vector: pT7T3D-PacI; Site_1: Not I; Site_2: Eco RI;
                     1st strand cDNA was primed with a Not I - oligo(dT) primer
                     [5'
                     TGTTACCAATCTGAAGTGGGAGCGGCCGCGGAAATTTTTTTTTTTTTTTTTTTTTTTT
                     T 3'], on equal amounts of mRNA from 2 13.5dpc and 2
                     14.5dpc embryos [total RNA provided by Minoru Ko, Wayne
                     State Univ., from 2 ]; double-stranded cDNA was ligated to
                     Eco RI adaptors (Pharmacia), digested with Not I and
                     cloned into the Not I and Eco RI sites of the modified
                     pT7T3 vector. Library went through one round of
                     normalization, and was constructed by Bento Soares and
                     M.Fatima Bonaldo."
ORIGIN      
        1 cgcggcacac ggagccaaca tgacgaatta acacagtggc tttggggact ggctctcctg
       61 ggctctgctg ggctgccctg accatgggag cactgggctt ggagttgcct ttcccctgcc
      121 gagaggtcct gtggccactg cctgcctacc tgttggtctc cgctggctgc tatgccctgg
      181 gcacggtggg ctatcgcgta ctacatttca cgactgcgag gacgctgccc gagagctgca
      241 gaccagatcg tggaggcccg agctgattta gcacgcaggg ggctgcgctt ctgacaccta
      301 aacccattcc agtctggaca tctttcctca ttccgcatta aaatgcagtt ttattttcta
      361 aaaaaaaaaa aaaaaaaaaa aaaaatttcc
//
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