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GenBank: AA032906.1
FASTA Graphics
LOCUS AA032906 390 bp mRNA linear EST 22-AUG-1996 DEFINITION mi22a08.r1 Soares mouse embryo NbME13.5 14.5 Mus musculus cDNA clone IMAGE:464246 5', mRNA sequence. ACCESSION AA032906 VERSION AA032906.1 DBLINK BioSample: SAMN00155048 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 390) AUTHORS Marra,M., Hillier,L., Allen,M., Bowles,M., Dietrich,N., Dubuque,T., Geisel,S., Kucaba,T., Lacy,M., Le,M., Martin,J., Morris,M., Schellenberg,K., Steptoe,M., Tan,F., Underwood,K., Moore,B., Theising,B., Wylie,T., Lennon,G., Soares,B., Wilson,R. and Waterston,R. TITLE The WashU-HHMI Mouse EST Project JOURNAL Unpublished COMMENT Contact: Marra M/Mouse EST Project WashU-HHMI Mouse EST Project Washington University School of MedicineP 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108 Tel: 314 286 1800 Fax: 314 286 1810 Email: mouseest@watson.wustl.edu This clone is available royalty-free through LLNL ; contact the IMAGE Consortium (info@image.llnl.gov) for further information. MGI:278062 Seq primer: -28M13 rev2 from Amersham. FEATURES Location/Qualifiers source 1..390 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="taxon:10090" /clone="IMAGE:464246" /sex="unknown" /tissue_type="embryo" /clone_lib="SAMN00155048 Soares mouse embryo NbME13.5 14.5" /dev_stage="13.5-14.5dpc total fetus" /lab_host="DH10B" /note="Vector: pT7T3D-PacI; Site_1: Not I; Site_2: Eco RI; 1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCGGAAATTTTTTTTTTTTTTTTTTTTTTTT T 3'], on equal amounts of mRNA from 2 13.5dpc and 2 14.5dpc embryos [total RNA provided by Minoru Ko, Wayne State Univ., from 2 ]; double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library went through one round of normalization, and was constructed by Bento Soares and M.Fatima Bonaldo." ORIGIN 1 cgcggcacac ggagccaaca tgacgaatta acacagtggc tttggggact ggctctcctg 61 ggctctgctg ggctgccctg accatgggag cactgggctt ggagttgcct ttcccctgcc 121 gagaggtcct gtggccactg cctgcctacc tgttggtctc cgctggctgc tatgccctgg 181 gcacggtggg ctatcgcgta ctacatttca cgactgcgag gacgctgccc gagagctgca 241 gaccagatcg tggaggcccg agctgattta gcacgcaggg ggctgcgctt ctgacaccta 301 aacccattcc agtctggaca tctttcctca ttccgcatta aaatgcagtt ttattttcta 361 aaaaaaaaaa aaaaaaaaaa aaaaatttcc //
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