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Mus musculus cDNA, clone:Y1G0104C20, strand:minus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000010254, based on BLAT search

GenBank: AK190896.1

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LOCUS       AK190896                 232 bp    mRNA    linear   HTC 19-SEP-2008
DEFINITION  Mus musculus cDNA, clone:Y1G0104C20, strand:minus,
            reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000010254, based
            on BLAT search.
ACCESSION   AK190896
VERSION     AK190896.1
KEYWORDS    HTC; ASSETS.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1
  AUTHORS   Watahiki,A., Waki,K., Hayatsu,N., Shiraki,T., Kondo,S.,
            Nakamura,M., Sasaki,D., Arakawa,T., Kawai,J., Harbers,M.,
            Hayashizaki,Y. and Carninci,P.
  TITLE     Libraries enriched for alternatively spliced exons reveal splicing
            patterns in melanocytes and melanomas
  JOURNAL   Nat. Methods 1 (3), 233-239 (2004)
   PUBMED   15782199
REFERENCE   2  (bases 1 to 232)
  AUTHORS   Arakawa,T., Carninci,P., Fukuda,S., Harbers,M., Hayatsu,N.,
            Hori,F., Imotani,K., Kawai,J., Kondo,S., Murata,M., Nakamura,M.,
            Nomura,K., Ohno,M., Sasaki,D., Shiraki,T., Waki,K., Watahiki,A. and
            Hayashizaki,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-SEP-2004) Contact:Yoshihide Hayashizaki The Institute
            of Physical and Chemical Research (RIKEN), Omics Science Center,
            RIKEN Yokohama Institute; 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama,
            Kanagawa 230-0045, Japan URL :http://www.osc.riken.jp/
COMMENT     Alternative Splicing Libraries (ASLs) are prepared by: Preparating
            of single-stranded DNA using a RNA template from full length cDNA
            libraries, hybridizating of
            single-stranded DNAs, removing of remaining single-stranded DNA,
            digesting of regions comprising double-stranded DNA by a set of 4
            bp-cutters, capturing of DNA hybrids with loop structures
            (alternative spliced exon), ligating of Y-shaped primers to
            isolated DNA hybrids with loop structures, PCR amplification of
            ligation products and their cloning into pFLCI vector. (Reference).
FEATURES             Location/Qualifiers
     source          1..232
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10090"
                     /clone="Y1G0104C20"
                     /cell_line="mixture of B16-F10Y and melan-c"
                     /cell_type="mixture of melanoma cell and melanocyte cell"
                     /clone_lib="Alternative Splicing Library L1"
                     /note="strand:minus, reference:ENSEMBL:Mouse-Transcript-
                     ENST:ENSMUST00000010254, based on BLAT search"
ORIGIN      
        1 cggcatctgc agacccattc taacaccagt gtggtttcgt tgcagtcaaa gctggcttct
       61 atgtccaatg acttcaagtc agttctagaa gtgaggactg agaatctgaa acagcagagg
      121 aaccgtcggg aacagttctc cagggcacca gtgtcagccc tgcctcttgc ccccaacaac
      181 cttggaggtg gtcccataat tttgggagca gagtcccgag cttccaggga cg
//
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