LOCUS AK190896 232 bp mRNA linear HTC 19-SEP-2008
DEFINITION Mus musculus cDNA, clone:Y1G0104C20, strand:minus,
reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000010254, based
on BLAT search.
ACCESSION AK190896
VERSION AK190896.1
KEYWORDS HTC; ASSETS.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1
AUTHORS Watahiki,A., Waki,K., Hayatsu,N., Shiraki,T., Kondo,S.,
Nakamura,M., Sasaki,D., Arakawa,T., Kawai,J., Harbers,M.,
Hayashizaki,Y. and Carninci,P.
TITLE Libraries enriched for alternatively spliced exons reveal splicing
patterns in melanocytes and melanomas
JOURNAL Nat. Methods 1 (3), 233-239 (2004)
PUBMED 15782199
REFERENCE 2 (bases 1 to 232)
AUTHORS Arakawa,T., Carninci,P., Fukuda,S., Harbers,M., Hayatsu,N.,
Hori,F., Imotani,K., Kawai,J., Kondo,S., Murata,M., Nakamura,M.,
Nomura,K., Ohno,M., Sasaki,D., Shiraki,T., Waki,K., Watahiki,A. and
Hayashizaki,Y.
TITLE Direct Submission
JOURNAL Submitted (15-SEP-2004) Contact:Yoshihide Hayashizaki The Institute
of Physical and Chemical Research (RIKEN), Omics Science Center,
RIKEN Yokohama Institute; 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama,
Kanagawa 230-0045, Japan URL :http://www.osc.riken.jp/
COMMENT Alternative Splicing Libraries (ASLs) are prepared by: Preparating
of single-stranded DNA using a RNA template from full length cDNA
libraries, hybridizating of
single-stranded DNAs, removing of remaining single-stranded DNA,
digesting of regions comprising double-stranded DNA by a set of 4
bp-cutters, capturing of DNA hybrids with loop structures
(alternative spliced exon), ligating of Y-shaped primers to
isolated DNA hybrids with loop structures, PCR amplification of
ligation products and their cloning into pFLCI vector. (Reference).
FEATURES Location/Qualifiers
source 1..232
/organism="Mus musculus"
/mol_type="mRNA"
/db_xref="taxon:10090"
/clone="Y1G0104C20"
/cell_line="mixture of B16-F10Y and melan-c"
/cell_type="mixture of melanoma cell and melanocyte cell"
/clone_lib="Alternative Splicing Library L1"
/note="strand:minus, reference:ENSEMBL:Mouse-Transcript-
ENST:ENSMUST00000010254, based on BLAT search"
ORIGIN
1 cggcatctgc agacccattc taacaccagt gtggtttcgt tgcagtcaaa gctggcttct
61 atgtccaatg acttcaagtc agttctagaa gtgaggactg agaatctgaa acagcagagg
121 aaccgtcggg aacagttctc cagggcacca gtgtcagccc tgcctcttgc ccccaacaac
181 cttggaggtg gtcccataat tttgggagca gagtcccgag cttccaggga cg
//