LOCUS NM_020133 7923 bp mRNA linear PRI 06-JUN-2024
DEFINITION Homo sapiens 1-acylglycerol-3-phosphate O-acyltransferase 4
(AGPAT4), mRNA.
ACCESSION NM_020133
VERSION NM_020133.3
KEYWORDS RefSeq; MANE Select.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 7923)
AUTHORS Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
TITLE A reference map of the human binary protein interactome
JOURNAL Nature 580 (7803), 402-408 (2020)
PUBMED 32296183
REFERENCE 2 (bases 1 to 7923)
AUTHORS Zhang,D., Shi,R., Xiang,W., Kang,X., Tang,B., Li,C., Gao,L.,
Zhang,X., Zhang,L., Dai,R. and Miao,H.
TITLE The Agpat4/LPA axis in colorectal cancer cells regulates antitumor
responses via p38/p65 signaling in macrophages
JOURNAL Signal Transduct Target Ther 5 (1), 24 (2020)
PUBMED 32296017
REMARK GeneRIF: The Agpat4/LPA axis in colorectal cancer cells regulates
antitumor responses via p38/p65 signaling in macrophages.
Publication Status: Online-Only
REFERENCE 3 (bases 1 to 7923)
AUTHORS Bradley,R.M., Marvyn,P.M., Aristizabal Henao,J.J., Mardian,E.B.,
George,S., Aucoin,M.G., Stark,K.D. and Duncan,R.E.
TITLE Acylglycerophosphate acyltransferase 4 (AGPAT4) is a mitochondrial
lysophosphatidic acid acyltransferase that regulates brain
phosphatidylcholine, phosphatidylethanolamine, and
phosphatidylinositol levels
JOURNAL Biochim Biophys Acta 1851 (12), 1566-1576 (2015)
PUBMED 26417903
REFERENCE 4 (bases 1 to 7923)
AUTHORS Schosser,A., Butler,A.W., Uher,R., Ng,M.Y., Cohen-Woods,S.,
Craddock,N., Owen,M.J., Korszun,A., Gill,M., Rice,J., Hauser,J.,
Henigsberg,N., Maier,W., Mors,O., Placentino,A., Rietschel,M.,
Souery,D., Preisig,M., Craig,I.W., Farmer,A.E., Lewis,C.M. and
McGuffin,P.
TITLE Genome-wide association study of co-occurring anxiety in major
depression
JOURNAL World J Biol Psychiatry 14 (8), 611-621 (2013)
PUBMED 24047446
REFERENCE 5 (bases 1 to 7923)
AUTHORS Wu,C., Ma,M.H., Brown,K.R., Geisler,M., Li,L., Tzeng,E., Jia,C.Y.,
Jurisica,I. and Li,S.S.
TITLE Systematic identification of SH3 domain-mediated human
protein-protein interactions by peptide array target screening
JOURNAL Proteomics 7 (11), 1775-1785 (2007)
PUBMED 17474147
REFERENCE 6 (bases 1 to 7923)
AUTHORS Lu,B., Jiang,Y.J., Zhou,Y., Xu,F.Y., Hatch,G.M. and Choy,P.C.
TITLE Cloning and characterization of murine 1-acyl-sn-glycerol
3-phosphate acyltransferases and their regulation by PPARalpha in
murine heart
JOURNAL Biochem J 385 (Pt 2), 469-477 (2005)
PUBMED 15367102
REFERENCE 7 (bases 1 to 7923)
AUTHORS Mungall,A.J., Palmer,S.A., Sims,S.K., Edwards,C.A., Ashurst,J.L.,
Wilming,L., Jones,M.C., Horton,R., Hunt,S.E., Scott,C.E.,
Gilbert,J.G., Clamp,M.E., Bethel,G., Milne,S., Ainscough,R.,
Almeida,J.P., Ambrose,K.D., Andrews,T.D., Ashwell,R.I.,
Babbage,A.K., Bagguley,C.L., Bailey,J., Banerjee,R., Barker,D.J.,
Barlow,K.F., Bates,K., Beare,D.M., Beasley,H., Beasley,O.,
Bird,C.P., Blakey,S., Bray-Allen,S., Brook,J., Brown,A.J.,
Brown,J.Y., Burford,D.C., Burrill,W., Burton,J., Carder,C.,
Carter,N.P., Chapman,J.C., Clark,S.Y., Clark,G., Clee,C.M.,
Clegg,S., Cobley,V., Collier,R.E., Collins,J.E., Colman,L.K.,
Corby,N.R., Coville,G.J., Culley,K.M., Dhami,P., Davies,J.,
Dunn,M., Earthrowl,M.E., Ellington,A.E., Evans,K.A., Faulkner,L.,
Francis,M.D., Frankish,A., Frankland,J., French,L., Garner,P.,
Garnett,J., Ghori,M.J., Gilby,L.M., Gillson,C.J., Glithero,R.J.,
Grafham,D.V., Grant,M., Gribble,S., Griffiths,C., Griffiths,M.,
Hall,R., Halls,K.S., Hammond,S., Harley,J.L., Hart,E.A.,
Heath,P.D., Heathcott,R., Holmes,S.J., Howden,P.J., Howe,K.L.,
Howell,G.R., Huckle,E., Humphray,S.J., Humphries,M.D., Hunt,A.R.,
Johnson,C.M., Joy,A.A., Kay,M., Keenan,S.J., Kimberley,A.M.,
King,A., Laird,G.K., Langford,C., Lawlor,S., Leongamornlert,D.A.,
Leversha,M., Lloyd,C.R., Lloyd,D.M., Loveland,J.E., Lovell,J.,
Martin,S., Mashreghi-Mohammadi,M., Maslen,G.L., Matthews,L.,
McCann,O.T., McLaren,S.J., McLay,K., McMurray,A., Moore,M.J.,
Mullikin,J.C., Niblett,D., Nickerson,T., Novik,K.L., Oliver,K.,
Overton-Larty,E.K., Parker,A., Patel,R., Pearce,A.V., Peck,A.I.,
Phillimore,B., Phillips,S., Plumb,R.W., Porter,K.M., Ramsey,Y.,
Ranby,S.A., Rice,C.M., Ross,M.T., Searle,S.M., Sehra,H.K.,
Sheridan,E., Skuce,C.D., Smith,S., Smith,M., Spraggon,L.,
Squares,S.L., Steward,C.A., Sycamore,N., Tamlyn-Hall,G., Tester,J.,
Theaker,A.J., Thomas,D.W., Thorpe,A., Tracey,A., Tromans,A.,
Tubby,B., Wall,M., Wallis,J.M., West,A.P., White,S.S.,
Whitehead,S.L., Whittaker,H., Wild,A., Willey,D.J., Wilmer,T.E.,
Wood,J.M., Wray,P.W., Wyatt,J.C., Young,L., Younger,R.M.,
Bentley,D.R., Coulson,A., Durbin,R., Hubbard,T., Sulston,J.E.,
Dunham,I., Rogers,J. and Beck,S.
TITLE The DNA sequence and analysis of human chromosome 6
JOURNAL Nature 425 (6960), 805-811 (2003)
PUBMED 14574404
REFERENCE 8 (bases 1 to 7923)
AUTHORS Clark,H.F., Gurney,A.L., Abaya,E., Baker,K., Baldwin,D., Brush,J.,
Chen,J., Chow,B., Chui,C., Crowley,C., Currell,B., Deuel,B.,
Dowd,P., Eaton,D., Foster,J., Grimaldi,C., Gu,Q., Hass,P.E.,
Heldens,S., Huang,A., Kim,H.S., Klimowski,L., Jin,Y., Johnson,S.,
Lee,J., Lewis,L., Liao,D., Mark,M., Robbie,E., Sanchez,C.,
Schoenfeld,J., Seshagiri,S., Simmons,L., Singh,J., Smith,V.,
Stinson,J., Vagts,A., Vandlen,R., Watanabe,C., Wieand,D., Woods,K.,
Xie,M.H., Yansura,D., Yi,S., Yu,G., Yuan,J., Zhang,M., Zhang,Z.,
Goddard,A., Wood,W.I., Godowski,P. and Gray,A.
TITLE The secreted protein discovery initiative (SPDI), a large-scale
effort to identify novel human secreted and transmembrane proteins:
a bioinformatics assessment
JOURNAL Genome Res 13 (10), 2265-2270 (2003)
PUBMED 12975309
REMARK Erratum:[Genome Res. 2003 Dec;13(12):2759]
REFERENCE 9 (bases 1 to 7923)
AUTHORS Leung,D.W.
TITLE The structure and functions of human lysophosphatidic acid
acyltransferases
JOURNAL Front Biosci 6, D944-D953 (2001)
PUBMED 11487472
REMARK Review article
Publication Status: Online-Only
REFERENCE 10 (bases 1 to 7923)
AUTHORS Dominguez,O., Ashhab,Y., Sabater,L., Belloso,E., Caro,P. and
Pujol-Borrell,R.
TITLE Cloning of ARE-containing genes by AU-motif-directed display
JOURNAL Genomics 54 (2), 278-286 (1998)
PUBMED 9828130
COMMENT REVIEWED REFSEQ: This record has been curated by NCBI staff. The
reference sequence was derived from BQ072907.1, AF156776.1,
AL109942.13 and AL122123.1.
On Nov 22, 2018 this sequence version replaced NM_020133.2.
Summary: This gene encodes a member of the
1-acylglycerol-3-phosphate O-acyltransferase family. This integral
membrane protein converts lysophosphatidic acid to phosphatidic
acid, the second step in de novo phospholipid biosynthesis.
[provided by RefSeq, Jul 2008].
##Evidence-Data-START##
Transcript exon combination :: SRR14038194.2059716.1,
SRR1660803.159285.1 [ECO:0000332]
RNAseq introns :: mixed sample support SAMEA1965299,
SAMEA1966682 [ECO:0006172]
##Evidence-Data-END##
##RefSeq-Attributes-START##
MANE Ensembl match :: ENST00000320285.9/ ENSP00000314036.4
RefSeq Select criteria :: based on single protein-coding transcript
##RefSeq-Attributes-END##
COMPLETENESS: full length.
PRIMARY REFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP
1-56 BQ072907.1 15-70
57-1815 AF156776.1 1-1759
1816-7864 AL109942.13 20526-26574 c
7865-7865 AL122123.1 5272-5272
7866-7923 AL109942.13 20467-20524 c
FEATURES Location/Qualifiers
source 1..7923
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/chromosome="6"
/map="6q26"
gene 1..7923
/gene="AGPAT4"
/gene_synonym="1-AGPAT4; dJ473J16.2; LPAAT-delta; LPAAT4;
LPLAT4"
/note="1-acylglycerol-3-phosphate O-acyltransferase 4"
/db_xref="GeneID:56895"
/db_xref="HGNC:HGNC:20885"
/db_xref="MIM:614795"
exon 1..124
/gene="AGPAT4"
/gene_synonym="1-AGPAT4; dJ473J16.2; LPAAT-delta; LPAAT4;
LPLAT4"
/inference="alignment:Splign:2.1.0"
exon 125..391
/gene="AGPAT4"
/gene_synonym="1-AGPAT4; dJ473J16.2; LPAAT-delta; LPAAT4;
LPLAT4"
/inference="alignment:Splign:2.1.0"
misc_feature 196..198
/gene="AGPAT4"
/gene_synonym="1-AGPAT4; dJ473J16.2; LPAAT-delta; LPAAT4;
LPLAT4"
/note="upstream in-frame stop codon"
CDS 214..1350
/gene="AGPAT4"
/gene_synonym="1-AGPAT4; dJ473J16.2; LPAAT-delta; LPAAT4;
LPLAT4"
/EC_number="2.3.1.51"
/note="1-AGP acyltransferase 4; lysophosphatidic acid
acyltransferase-delta (LPAAT-delta); 1-AGPAT 4;
lysophosphatidic acid acyltransferase delta;
1-acylglycerol-3-phosphate O-acyltransferase 4
(lysophosphatidic acid acyltransferase, delta);
lysophospholipid acyltransferase 4"
/codon_start=1
/product="1-acyl-sn-glycerol-3-phosphate acyltransferase
delta"
/protein_id="NP_064518.1"
/db_xref="CCDS:CCDS5280.1"
/db_xref="GeneID:56895"
/db_xref="HGNC:HGNC:20885"
/db_xref="MIM:614795"
/translation="MDLAGLLKSQFLCHLVFCYVFIASGLIINTIQLFTLLLWPINKQ
LFRKINCRLSYCISSQLVMLLEWWSGTECTIFTDPRAYLKYGKENAIVVLNHKFEIDF
LCGWSLSERFGLLGGSKVLAKKELAYVPIIGWMWYFTEMVFCSRKWEQDRKTVATSLQ
HLRDYPEKYFFLIHCEGTRFTEKKHEISMQVARAKGLPRLKHHLLPRTKGFAITVRSL
RNVVSAVYDCTLNFRNNENPTLLGVLNGKKYHADLYVRRIPLEDIPEDDDECSAWLHK
LYQEKDAFQEEYYRTGTFPETPMVPPRRPWTLVNWLFWASLVLYPFFQFLVSMIRSGS
SLTLASFILVFFVASVGVRWMIGVTEIDKGSAYGNSDSKQKLND"
misc_feature 244..306
/gene="AGPAT4"
/gene_synonym="1-AGPAT4; dJ473J16.2; LPAAT-delta; LPAAT4;
LPLAT4"
/note="propagated from UniProtKB/Swiss-Prot (Q9NRZ5.1);
transmembrane region"
misc_feature 499..516
/gene="AGPAT4"
/gene_synonym="1-AGPAT4; dJ473J16.2; LPAAT-delta; LPAAT4;
LPLAT4"
/note="propagated from UniProtKB/Swiss-Prot (Q9NRZ5.1);
Region: HXXXXD motif.
/evidence=ECO:0000250|UniProtKB:Q9D517"
misc_feature 586..648
/gene="AGPAT4"
/gene_synonym="1-AGPAT4; dJ473J16.2; LPAAT-delta; LPAAT4;
LPLAT4"
/note="propagated from UniProtKB/Swiss-Prot (Q9NRZ5.1);
transmembrane region"
misc_feature 1132..1194
/gene="AGPAT4"
/gene_synonym="1-AGPAT4; dJ473J16.2; LPAAT-delta; LPAAT4;
LPLAT4"
/note="propagated from UniProtKB/Swiss-Prot (Q9NRZ5.1);
transmembrane region"
misc_feature 1225..1287
/gene="AGPAT4"
/gene_synonym="1-AGPAT4; dJ473J16.2; LPAAT-delta; LPAAT4;
LPLAT4"
/note="propagated from UniProtKB/Swiss-Prot (Q9NRZ5.1);
transmembrane region"
exon 392..561
/gene="AGPAT4"
/gene_synonym="1-AGPAT4; dJ473J16.2; LPAAT-delta; LPAAT4;
LPLAT4"
/inference="alignment:Splign:2.1.0"
exon 562..723
/gene="AGPAT4"
/gene_synonym="1-AGPAT4; dJ473J16.2; LPAAT-delta; LPAAT4;
LPLAT4"
/inference="alignment:Splign:2.1.0"
exon 724..877
/gene="AGPAT4"
/gene_synonym="1-AGPAT4; dJ473J16.2; LPAAT-delta; LPAAT4;
LPLAT4"
/inference="alignment:Splign:2.1.0"
exon 878..980
/gene="AGPAT4"
/gene_synonym="1-AGPAT4; dJ473J16.2; LPAAT-delta; LPAAT4;
LPLAT4"
/inference="alignment:Splign:2.1.0"
exon 981..1056
/gene="AGPAT4"
/gene_synonym="1-AGPAT4; dJ473J16.2; LPAAT-delta; LPAAT4;
LPLAT4"
/inference="alignment:Splign:2.1.0"
exon 1057..1255
/gene="AGPAT4"
/gene_synonym="1-AGPAT4; dJ473J16.2; LPAAT-delta; LPAAT4;
LPLAT4"
/inference="alignment:Splign:2.1.0"
exon 1256..7923
/gene="AGPAT4"
/gene_synonym="1-AGPAT4; dJ473J16.2; LPAAT-delta; LPAAT4;
LPLAT4"
/inference="alignment:Splign:2.1.0"
regulatory 1795..1800
/regulatory_class="polyA_signal_sequence"
/gene="AGPAT4"
/gene_synonym="1-AGPAT4; dJ473J16.2; LPAAT-delta; LPAAT4;
LPLAT4"
/note="hexamer: ATTAAA"
polyA_site 1816
/gene="AGPAT4"
/gene_synonym="1-AGPAT4; dJ473J16.2; LPAAT-delta; LPAAT4;
LPLAT4"
/note="major polyA site"
regulatory 7839..7844
/regulatory_class="polyA_signal_sequence"
/gene="AGPAT4"
/gene_synonym="1-AGPAT4; dJ473J16.2; LPAAT-delta; LPAAT4;
LPLAT4"
/note="hexamer: AATAAA"
polyA_site 7860
/gene="AGPAT4"
/gene_synonym="1-AGPAT4; dJ473J16.2; LPAAT-delta; LPAAT4;
LPLAT4"
regulatory 7891..7896
/regulatory_class="polyA_signal_sequence"
/gene="AGPAT4"
/gene_synonym="1-AGPAT4; dJ473J16.2; LPAAT-delta; LPAAT4;
LPLAT4"
/note="hexamer: AATAAA"
polyA_site 7923
/gene="AGPAT4"
/gene_synonym="1-AGPAT4; dJ473J16.2; LPAAT-delta; LPAAT4;
LPLAT4"
ORIGIN
1 gctttttctt tccagtgttg gctgacttac agctcttata aactagtggc aatttctgaa
61 cccagccggc tccatctcag cttctggttt ctaagtccat gtgccaaagg ctgccaggaa
121 ggagacgcct tcctgagtcc tggatctttc ttccttctgg aaatctttga ctgtgggtag
181 ttatttattt ctgaataaga gcgtccacgc atcatggacc tcgcgggact gctgaagtct
241 cagttcctgt gccacctggt cttctgctac gtctttattg cctcagggct aatcatcaac
301 accattcagc tcttcactct cctcctctgg cccattaaca agcagctctt ccggaagatc
361 aactgcagac tgtcctattg catctcaagc cagctggtga tgctgctgga gtggtggtcg
421 ggcacggaat gcaccatctt cacggacccg cgcgcctacc tcaagtatgg gaaggaaaat
481 gccatcgtgg ttctcaacca caagtttgaa attgactttc tgtgtggctg gagcctgtcc
541 gaacgctttg ggctgttagg gggctccaag gtcctggcca agaaagagct ggcctatgtc
601 ccaattatcg gctggatgtg gtacttcacc gagatggtct tctgttcgcg caagtgggag
661 caggatcgca agacggttgc caccagtttg cagcacctcc gggactaccc cgagaagtat
721 tttttcctga ttcactgtga gggcacacgg ttcacggaga agaagcatga gatcagcatg
781 caggtggccc gggccaaggg gctgcctcgc ctcaagcatc acctgttgcc acgaaccaag
841 ggcttcgcca tcaccgtgag gagcttgaga aatgtagttt cagctgtata tgactgtaca
901 ctcaatttca gaaataatga aaatccaaca ctgctgggag tcctaaacgg aaagaaatac
961 catgcagatt tgtatgttag gaggatccca ctggaagaca tccctgaaga cgatgacgag
1021 tgctcggcct ggctgcacaa gctctaccag gagaaggatg cctttcagga ggagtactac
1081 aggacgggca ccttcccaga gacgcccatg gtgccccccc ggcggccctg gaccctcgtg
1141 aactggctgt tttgggcctc gctggtgctc taccctttct tccagttcct ggtcagcatg
1201 atcaggagcg ggtcttccct gacgctggcc agcttcatcc tcgtcttctt tgtggcctcc
1261 gtgggagttc gatggatgat tggtgtgacg gaaattgaca agggctctgc ctacggcaac
1321 tctgacagca agcagaaact gaatgactga ctcagggagg tgtcaccatc cgaagggaac
1381 cttggggaac tggtggcctc tgcatatcct ccttagtggg acacggtgac aaaggctggg
1441 tgagcccctg ctgggcacgg cggaagtcac gacctctcca gccagggagt ctggtctcaa
1501 ggccggatgg ggaggaagat gttttgtaat ctttttttcc ccatgtgctt tagtgggctt
1561 tggttttctt tttgtgcgag tgtgtgtgag aatggctgtg tggtgagtgt gaactttgtt
1621 ctgtgatcat agaaagggta ttttaggctg caggggaggg cagggctggg gaccgaaggg
1681 gacaagttcc cctttcatcc tttggtgctg agttttctgt aacccttggt tgccagagat
1741 aaagtgaaaa gtgctttagg tgagatgact aaattatgcc tccaagaaaa aaaaattaaa
1801 gtgcttttct gggtcatctg tggtgtatgt tgacatggac cgctgcccct ccatcctgcc
1861 cttgcccgtg gctttggtgt ttcagatcct actcacggga ggcagctgcc gcggaggtgt
1921 gggcagctga gaggggtggg caggccagca cagctcctgg caggagacac aggtccagga
1981 gcccgtgagc atttgtgaga gcagagatgg caagcacgtg cgtggagacc aacgaagcgt
2041 gtccctggca cacagcaagg gagagtttcg ctcggtctct gcagtgcagt ctcctggcgt
2101 aagtcttgaa gatggaccca actccctgaa gaaatggatc ttgaaattga acacaaacat
2161 catgaacgta cagcctgggc attctgcagt gatttgtgag atgagcttgc atcgactgtt
2221 cctctcagca gtgacagcca aagtcacccc tgataaaatg cagtttcact ttataaaatg
2281 cctcgcccct tcccagcatg tagagcttgt ctgcagccct aaaggagcca ggtcctgctc
2341 tgatgagggt caaatcagag ccgacatccc atgtacaaaa aacccccagg cttctgcctt
2401 ccttccgggc tcctgtctgc tcccatttta ctccacggaa tagttctgcc ctgtagcatc
2461 tccagcggcc tttgcagact gctgtcagcc aacaggtgcc aaaaatgcaa aagaagaaga
2521 aactaaaagc agtgctattt ttgcagaaca aatgctagct attaaatcat cttaggccaa
2581 gacaccaatg gcacttggac cgataggtat ttggagcatg agttaggaag gatatgtctt
2641 tctccaggtg cgcagctgaa agttgctgag aaaagctgcc tgtgttgctg tggtaacatg
2701 agaaggaatg aacagctcta tacaaaaact ggccacacat tataaggcct attctatgca
2761 gttatgtttt tggctatttg ctcatttagt cattgtttat caaccaaact aggtaagcca
2821 aggagttaca agacagctct agagaagctc cctctgctta caggacaata atgcattgtt
2881 tgtttgttga tacaaatgaa ttatttgaat ccacagaagt gaaaaaggga aaaaatcctg
2941 gctgacttgt gggaagagcg tactgcagag cctagggcct gagttaactc gctggcggcc
3001 ctgcaggcag gcttgatggg agcgcccagc acatctgccc accctgcaca gggcatgaag
3061 caccgtgtgc agagatctgt gggcaaaagc ccaggacgtg accttaggca agaggggtcc
3121 ttggagaagt ggaggtgtgc atggtgtgta gccttgcgtg tctgtgagca cgccctagtg
3181 gacctgcgcc tggattatcg ggccagtctc tcggaggggt ccgtgatgct gagttacgcc
3241 attcacctga actctgcagc tcatcggctg taggatttgg gtgagacact tttccttgct
3301 gactcatttc cccatctgca aaatgggggt gacgatatct acctcttggg gtgtgtcaag
3361 ggtaaaagag cacactttaa gaatcctaaa gcactaagcc aaggatgaaa gactagaaag
3421 ctgcctctat gttgctactc tcaaaaatgc tcagaaattt tcttgtcgta ctaaaaatgt
3481 gttatttttc tcattttcat tcttccattc aacacagaag taatctaagt aagttctaaa
3541 aacatagatc cgggggccca cagacattca attttcttct gaattggctc tggtatcaca
3601 caacacacaa gcaagcaaac agcctgcctg ctagggcctt gggttccagg ctggaggaat
3661 gcaggcagaa atctcttagg aaataactaa gtgagcgcca agttttgaat tgccttagag
3721 ccgaaagaga atgtggcgtg ctggggagag atctggacct ctttctcatc agtcatttga
3781 cctgtccacg aagaacaatt ttgttatgca gcctgggaac ctggtttcct tacaccctgc
3841 gcccttaatg acacagacaa ccacgggtac tcctggacgg aagctcatca cgcctcctca
3901 aataccttca cccatgttca ggtcacacgg aggtcaccat ctcctcagca cacaacaact
3961 gctttggttc ccagggttat tttttctacg agttgaaaac ccacggagcc taagccatct
4021 ttttgtcccc taaggagagc cccatagact aagagctaca cttcctgtct gtctttccca
4081 cctttccatc tcattctagc ttaaagctta tcacgctact gagagcctgt tctgtagttc
4141 aaggtggcag agaaaaatac gaaccttgag tgaaatctgg agagcagtgc agggtcatcc
4201 ccagacgtga cgggccatat cctaggcgac tcctgcagct tatagaagca gggaatttaa
4261 tggacttctt ccaggaaagc cagcccacgg ggactcttaa aaagacacca accatttgga
4321 agatgcagct tgaggtctgg gggactggac tgattctgcc accatatagt gaacacgatt
4381 aatcgggagg aagaagtaag gcactctgtg agttagtcta ccaccctatt gactttgaca
4441 gagagaaaac caactttttt catatgatca cacagtagta ctggaatatc tcgactgcaa
4501 tatcctctaa acaaatgcat gcaaagcttt gtaaaagcag ctgatgatta aaaaaatttt
4561 aaaacattag ggtttttcta atatatccat atgtgggaca gtgatttaaa tgtcattaat
4621 tgtccagcca caacttgtaa cagtgatgtg aaagacacaa aaaaggcccc actggagtgg
4681 aggagtggtc tggctggtcc ccaagcaaaa cagaatgggc ttcgcatagg ctttagaact
4741 tattcataaa tattgaccaa agtgtgaagc aaggtatcca gtgaagattc tctagggtct
4801 ctacttctgc tcaagagatt taatccagct ttctctaaag cagtggtcca gacttgccac
4861 atactaggtt aaagataaag tgagaaaaac aaatactttg gtttgcacgt ctcttgaggg
4921 gccctaggct gtatggagga atatttagag gaatatttgt gagctgccat ttgtgtttaa
4981 aagaacatga aagcaagacg ggccctaacc cgcctgtgtc tgtagctgac caggaggcca
5041 ctgcttcctt cccccagagc ttctaggaga gccccccaaa atgcaactct ccaaggtcct
5101 gtggagtagt cccctcataa tgcctccacc acacctgtgt ctcacgatgc cttaactgac
5161 gtagctccta agtgatgttc acagagaagg aaatacttag gcaagcccat gtgtaacatg
5221 ctcacagatg tgctggcttt gccaatgctt tcggactcca ccatgctttc tgattcttct
5281 ggtcctagtc acgccttggc acggggcaga agcggccgag ctatgctgac aggtgctgct
5341 gagagccaac agccattcag ccgagaaggc gtttctaggt gagggcatga gagctggcat
5401 tccaggctgc tgagtgcggc atctgcaggt gcaccacatt gctccacggt gtgttcagaa
5461 gagaccagca tcttgaatct caggctctgg gctcattctt tgcctctgcc aagggaccat
5521 tcaggggctt caagccagct gctgaaaatc ccccttttct ggatttttcc ccatctatat
5581 aaggatatgg tggctgtgtg gacaagaaat tggtttcatc tcagaagggt gcttccctac
5641 aaagatcttc agaaatttaa ttgaaaacaa ttttttgaac tttttttgtt tggccaggat
5701 gaatgaagct gaagtcacat cctgaatgtg catgccagac aaagggtaag caagcagtct
5761 ttacaaatgc caatccctct gcactccaaa accggagccc gtaatgaagg cccaggttcc
5821 agtcatcaca ggatccccca agccagcaca gtgcctgatg cctccctagg cattagcaca
5881 tcccagcact ctaattcaca tcctcagcat ccctgcctcc tgcatccagg gcttaggttg
5941 agtgtttcca gagtgtcaag tacatttaaa cacaaaccaa atactccagg gttttgacct
6001 gtcacaatgg cttttgaaaa caacagacgt acaatttgag agaaggactc gatggacttc
6061 ttaggctgag gactaaggac atgttgctgt tcctcttggt ccaggacatt caataccatt
6121 cacagcattc atcaccaact gacatagtat cgatcacgac attcaactgt ttgaaattac
6181 tgctattttg gtcaaaaact aagaaatcag aaattgcacc tacatagtgt gtctcctcat
6241 tggagtccca tctctctgag cacagggatg ggctcctgag ctcacagccc tgccctgccc
6301 ccccagcagg gcctcaccct catggccctc agtgaatagt gactacatga agggggcact
6361 tactagaggc ctgagtaatg gaatttcaat tagcttctgc ttcccttttc taaaaaagaa
6421 ccacctttct taagaataat taggccaaaa taaacttgag taatttgtac agtcaatatt
6481 ttgaagtttc ggagttacca gtgaaatagc aaagctgttc tccaggaaag gacatccagg
6541 ttcccaccca ggccttcctt cctcagctcc tgcagcctca tcctagttta atatgaaggt
6601 tgtgaatggg ttaaaaagca gttcttaagc acctacttag atcttttcag tatctggaaa
6661 tagcacccag ggctgtgtta gtaaagcttt tcactgggta agagtcatac aatttttaca
6721 tttaatttgt ttttttaaaa caaggcagtc ttgttttaca tattggaatg actagatatt
6781 ttttgacatt tctggcacct ctggttctgc tgaattttaa gggaggtgag tggtatgtac
6841 acaaagttaa gattgctgca taataattcc tttgcatttc caaaataaat tacacaaatg
6901 tcacttttcc ttttctattc catagtacta gttttagcca gatctttgcc aaacattcta
6961 ggacattcag aaaagtcatc catggaattt tggaatgcca ttctctctgt atctttgcag
7021 atcatgagga aggaaagttg atgaactagt acgggatggc tcagacttgt aaagtgtcta
7081 agtcacaacg ctgcagagcc agcttccggg tagcaatggc atggcctcag ctgtgccaag
7141 gctacaactg gagcttcctt actcgcatct gaggcaaaca cagcaaggct ggcccaggcc
7201 gcccacagtc aacagatgtt cacgggagat ggatcagccc ctcttttgct tgttctatca
7261 aggaaagaga tctaaccatg ggcaagggtg gaacacaccc ggttcaggtc agggaatgag
7321 ttgctctaag tgaacagtga attcttactt ctcagcctgt atcaattgta cccagggttc
7381 atgattactg tgacccacta agggatgatc tttgaggaaa cagacctgga gggaggtaat
7441 ttaaggaggg aaagcgagtg ggttttacag actgctttgt ggcgacattc aggaacagac
7501 cattcaatgg atgacttttc agtacataaa agcaagcagt aatggccagg agagagcaaa
7561 gatgccctaa gaatgacatc agactgacca aatgtgcctt ttcaaataag ttatgagaac
7621 gaatgaattg gaattagagc aaggtattgg acagaaccat gctagtcttt gagagaacgt
7681 gtggattggg tggatgtttg gaccgaatga atggtaactt tttaatgtga aacagagaac
7741 tgatatatgg gctgtcatgt caccctaggg tactgtcgct ctgtcatgtg tagcaatctt
7801 tccaaatttt tgttggtggg ctgaacaaag ataaaactaa taaagtatgt gtggatacca
7861 ctagacagca cagcttactg gactggatgt aataaaactg actgaaactt taacttggaa
7921 aaa
//