|
| Status |
Public on Sep 01, 2012 |
| Title |
Adamts12 KO 2%DSS |
| Sample type |
RNA |
| |
|
| Source name |
distal colon from Adamts12 KO mouse after induction of ulcerative colitis with DSS
|
| Organism |
Mus musculus |
| Characteristics |
genotype: Adamts12 KO
tissue: distal colon
treatment: 2% DSS
|
| Treatment protocol |
2% DSS (dextran sodium sulfate) in drinking water during 8 days, followed by 1 day without treatment
|
| Growth protocol |
Mice were fed ad libitum and treatment started at 8-10 weeks of age
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA were hybridized on Affymetrix Mouse Gene 1.0 ST Array according to the protocols recommended by the manufacturer (Affymetrix, Santa Clara, CA, USA).
|
| Scan protocol |
Washing and scanning were performed using Fluidics Station 400 and GeneChip Scanner (Affymetrix Inc.).
|
| Description |
Gene expression data from distal colon of an Adamts12-KO mouse with ulcerative colitis
|
| Data processing |
After scanning, rawdata (Affymetrix Cel files) were processed with RMAExpress (http://RMAExpress.bmbolstad.com), using default settings
|
| |
|
| Submission date |
Aug 31, 2012 |
| Last update date |
Sep 01, 2012 |
| Contact name |
ANGELA MONCADA PAZOS |
| Organization name |
UNIVERSIDAD DE OVIEDO
|
| Street address |
FERNANDO BONGERA
|
| City |
OVIEDO |
| ZIP/Postal code |
33006 |
| Country |
Spain |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE40529 |
Expression data from untreated and DSS-treated Adamts12 WT and KO mice |
|
