|
| Status |
Public on Oct 01, 2013 |
| Title |
30 mins after birth neonates, aorta Ecs-rep3 |
| Sample type |
RNA |
| |
|
| Source name |
rat 30 mins after birth neonates, aorta Ecs
|
| Organism |
Rattus norvegicus |
| Characteristics |
cell type: vascular endothelial cells
tissue: aorta
strain: Wistar
developmental stage: 30 mins after birth neonate (day0)
|
| Treatment protocol |
Pooled tissues from the DA or the aorta were treated with collagenase-dispase enzyme mixture and reacted with FITC-conjugated anti-CD31 and APC/Cy7-conjugated anti-CD45 antibodies as cell surface markers for EC isolation using FACS.
|
| Growth protocol |
Rat fetuses at the 21st day of gestation (e21) and neonates 30 minutes after a delivery by cesarean section (day 0).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from the collected cells by RNeasy micro kit (QIAGEN) according to the manufacturer instructions.
|
| Label |
biotin
|
| Label protocol |
Samples were enzymatically fragmented and biotinylated using the WT Terminal Labeling Kit (Affymetrix)
|
| |
|
| Hybridization protocol |
Samples were hybridized using Affymetrix hybridization kit materials. Heat cocktails at 99° for 5 minutes, then 45° for 5 minutes centrifuge at max speed for 1 minute. Transfer 80μl of hyb solution to each array, then tape holes. Hybridize 16 hours at 45° at 60rpm. Fluidics Station 450.
|
| Scan protocol |
Affymetrix, GeneChip Scanner 3000 7G
|
| Description |
Gene expression data from endothelial cells in rat neonatal aorta
|
| Data processing |
Data were processed using GeneBASE software. MAT background correction were applied to probe intensities, gene level expression were summarized from the corrected intensities and then quantile normalized.
|
| |
|
| Submission date |
Aug 30, 2012 |
| Last update date |
Oct 01, 2013 |
| Contact name |
Norika Mengchia Liu |
| E-mail(s) |
[email protected]
|
| Organization name |
Waseda University
|
| Department |
Dep. Life science & Medical Bioscience
|
| Street address |
2-2, Wakamatsu-cho
|
| City |
Shinjuku-ku |
| State/province |
Tokyo |
| ZIP/Postal code |
162-8480 |
| Country |
Japan |
| |
|
| Platform ID |
GPL6247 |
| Series (1) |
| GSE40500 |
Expression data from endthelial cells in ductus arteriosus and aorta in rat fetuses or neonates |
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