|
| Status |
Public on Dec 05, 2012 |
| Title |
Bcl6 KO 2 sample B |
| Sample type |
RNA |
| |
|
| Source name |
Treg cell
|
| Organism |
Mus musculus |
| Characteristics |
genotype: Bcl6 knock-out
cell type: FoxP3+ Treg
|
| Treatment protocol |
Tregs were stimulated with anti-CD3 nd anti-CD28 for 16 hours prior to RNA extraction
|
| Extracted molecule |
total RNA |
| Extraction protocol |
standard RNA isolation method-- Qiagen Rneasy
|
| Label |
biotin
|
| Label protocol |
Biotinylated DNA were prepared according to the Affymetrix standard WT protocol using 100ng of total RNA (not pretreated with Ribominus (Invitrogen))
|
| |
|
| Hybridization protocol |
Labeled DNA were hybridized for 17 hr at 45C on Mouse Gene 1.0 St Genechips. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
Scanned using Affymetrix GeneChip® Scanner 3000 7G
|
| Data processing |
The data were analyzed with Partek Genomics Suite to produce RMA
|
| |
|
| Submission date |
Aug 30, 2012 |
| Last update date |
Dec 05, 2012 |
| Contact name |
Alexander Dent |
| E-mail(s) |
[email protected]
|
| Phone |
317 274-7524
|
| Organization name |
Alexander Dent
|
| Street address |
950 W. Walnut St. R2 302, Walther Oncology Center
|
| City |
Indianapolis |
| State/province |
IN |
| ZIP/Postal code |
46202 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE40493 |
Bcl6-deficient regulatory T cells |
|
