|
| Status |
Public on Aug 24, 2012 |
| Title |
control #4 |
| Sample type |
RNA |
| |
|
| Source name |
KLS cells
|
| Organism |
Mus musculus |
| Characteristics |
tissue: bone marrow
cell type: Lin- c-Kit+ Sca-1+
transduction: control
strain: C57Bl/6
|
| Treatment protocol |
c-Kit+ cells were transduced with lentiviurs expressing KLF7 or control virus. 72 hrs post-transduction, KLS cells were sorted and RNA prepared.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
KLS cells were sorted and RNA prepped used RNA XS columns (Machery-Nagel).
|
| Label |
biotin
|
| Label protocol |
RNA was amplified using the NuGen Ovation System.
|
| |
|
| Hybridization protocol |
Hybridization to Affymetrix Mouse Gene 1.0 ST array following manufacturer's recommendations
|
| Scan protocol |
Affymetrix software/protocol
|
| Data processing |
Data normalization using the Robust Multichip Average (RMA) algorithm and detection p-values were made using Affymetrix Expression Console software.
|
| |
|
| Submission date |
Aug 23, 2012 |
| Last update date |
Aug 24, 2012 |
| Contact name |
laura schuettpelz |
| E-mail(s) |
[email protected]
|
| Organization name |
washington university
|
| Street address |
660 S. Euclid
|
| City |
Saint Louis |
| State/province |
MO |
| ZIP/Postal code |
63110 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (2) |
| GSE40327 |
KLF7 overexpression in HSPCs expression array |
| GSE40331 |
Kruppel like factor 7 overexpression suppresses hematopoietic stem and progenitor cell function |
|
