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Sample GSM98276

Query DataSets for GSM98276

Status

Public on Feb 23, 2007

Title

5%sugar_HS_2

Sample type

RNA

Source name

mRNA

Organism

Saccharomyces cerevisiae

Characteristics

Commercial baker's yeast specilized for use in high-sugar dough.

Extracted molecule

polyA RNA

Extraction protocol

Each samples of total RNA were extracted using the hot phenol method. Poly (A)+ RNA was isolated from the total RNA by using an Oligotex® dt30 (super) mRNA purification kit (Takara, Japan) according to the manufacturer’s instructions.

Label

Biotin

Label protocol

Labeling was done according to the Affymetrix GeneChip® Expression Analysis Technical Manual.

Description

After 60min fermentation using 5% sucrose LF medium.

Data processing

Affymetrix GeneChip Operating Software Version 1.2

Submission date

Feb 23, 2006

Last update date

Feb 27, 2006

Contact name

Jun Shima

E-mail(s)

[email protected]

Organization name

National Food Research Institute

Department

Applied Microbiology

Lab

Yeast

Street address

1-2-12 Kannondai

City

Tsukuba

State/province

Ibaraki

ZIP/Postal code

305-8642

Country

Japan

Platform ID

GPL2529

Series (1)

GSE4295

Sugar stress during fermentation (baker's yeast)

Data table header descriptions
ID_REF
VALUE	Normalized value (Per Chip, Per Gene)
BKD_RAW	Control signal (calculated :GeneSpring)
SIGNAL_RAW	Raw intensities (measured: Affymetrix GeneChip)
ABS_CALL	Flag (Affymetrix)

Data table
ID_REF	VALUE	BKD_RAW	SIGNAL_RAW	ABS_CALL
1769308_at	0.376	69.38	26.07	A
1769309_at	0.993	20.34	20.19	A
1769310_at	0.526	20.34	10.7	A
1769311_at	1.815	1,476	2,679	P
1769312_at	1.004	179.8	180.6	P
1769313_at	0.452	116.3	52.55	P
1769314_at	1.558	2,911	4,533	P
1769315_at	0.0845	20.34	1.72	A
1769316_s_at	0.0627	20.34	1.275	A
1769317_at	0.388	705.8	273.6	P
1769318_at	0.0966	20.34	1.966	A
1769319_at	1.141	563	642.1	P
1769320_at	1.493	261.4	390.3	P
1769321_at	2.233	376	839.8	P
1769322_s_at	0.427	281.5	120.2	P
1769323_at	1.01	552.7	558	P
1769324_at	0.535	312.9	167.3	P
1769325_at	0.324	36.54	11.86	A
1769326_at	0.22	20.34	4.473	A
1769327_at	0.131	20.34	2.656	A

Total number of rows: 10928

Table truncated, full table size 331 Kbytes.

Supplementary file	Size	Download	File type/resource
GSM98276.CEL.gz	1.5 Mb	(ftp)(http)	CEL
GSM98276.CHP.gz	58.0 Kb	(ftp)(http)	CHP
Processed data provided as supplementary file