|
| Status |
Public on Jul 28, 2012 |
| Title |
OCSC, IL17,4h, rep1 |
| Sample type |
RNA |
| |
|
| Source name |
ovarian cancer stem cell, IL17,4h, replicate 1
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: A2780-derived ovarian cancer stem cell
|
| Treatment protocol |
ovarian cancer stem cells from A2780 cell line was stimulated by IL17A in the stem cell medium.The cultures were incubated for 4 or 6 hours at 37 ºC in a humidified incubator with 5% CO2.
|
| Growth protocol |
ovarian cancer stem cells from A2780 cell line was maintain in serum-free DMEM/F12 supplemented with 10 ng/mL basic fibroblast growth factor , 20 ng/mL epidermal growth factor , 5 μg/mL insulin, 0.4% bovine serum albumin.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using Trizol. Total RNA from each sample was quantified by the NanoDrop ND-1000 and RNA integrity was assessed by standard denaturing agarose gel electrophoresis
|
| Label |
Cy3
|
| Label protocol |
1 μg of total RNA from each sample was amplified and transcribed into fluorescent cRNA with using the manufacturer’s Agilent’s Quick Amp Labeling protocol (version 5.7, Agilent Technologies).
|
| |
|
| Hybridization protocol |
The labeled cRNAs were hybridized onto the Whole Genome Oligo Array (4x44K, Agilent Technologies).
|
| Scan protocol |
After having washed the slides, the arrays were scanned by the Agilent Scanner G2505B.
|
| Description |
Gene expression in ovarian cancer stem cells
|
| Data processing |
The scanned images were analyzed with Agilent Feature Extraction software (version 10.5.1.1).
Raw signal intensities were normalized in Median method by GeneSpring GX v11.5.1, and low intensity genes were filtered (Genes that at least 2 out of 3 samples have flags in Detected were chosen for further analysis).
|
| |
|
| Submission date |
Jul 27, 2012 |
| Last update date |
Jul 28, 2012 |
| Contact name |
tong xiang |
| E-mail(s) |
[email protected]
|
| Organization name |
xinqiao hospital
|
| Street address |
xinqiao
|
| City |
chongqing |
| ZIP/Postal code |
400000 |
| Country |
China |
| |
|
| Platform ID |
GPL6480 |
| Series (1) |
| GSE39698 |
Comparing control and IL17A treated A2780-derived ovarian cancer stem cell |
|
