|
| Status |
Public on Feb 16, 2006 |
| Title |
Patient-97-122_HR-CGH (Experiment-ID: 36230) |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
Genomic DNA isolated from a lymphoblast cell line
|
| Organism |
Homo sapiens |
| Characteristics |
Patient with Der22/ES; lymphoblast cell line.
|
| Extracted molecule |
genomic DNA |
| Label |
Cy3
|
| |
|
| Channel 2 |
| Source name |
Genomic DNA from 7 normal male individuals (Promega)
|
| Organism |
Homo sapiens |
| Characteristics |
Pooled genomic DNA from 7 normal male individuals (Promega); used as control.
|
| Extracted molecule |
genomic DNA |
| Label |
Cy5
|
| |
|
| |
| Description |
High resolution comparative genome hybridization (HR-CGH) was performed in order to identify the exact breakpoints of large aberrations causing the respective disease.
|
| Data processing |
For each spot on the array log2-ratios of the Cy3-labeled test sample versus the Cy5-labeled reference sample were calculated. Fluorescence intensity normalization was performed using the Qspline algorithm (Workman et al 2002), implemented as normalize.qspline in the Bioconductor package (www.bioconductor.org).
|
| |
|
| Submission date |
Feb 13, 2006 |
| Last update date |
Feb 15, 2006 |
| Contact name |
Jan Oliver Korbel |
| Phone |
+1 203 432 5405
|
| Fax |
+1 203 432 5175
|
| URL |
http://bioinfo.mbb.yale.edu/array/
|
| Organization name |
Yale University
|
| Street address |
266 Whitney Avenue
|
| City |
New Haven |
| State/province |
CT |
| ZIP/Postal code |
06520 |
| Country |
USA |
| |
|
| Platform ID |
GPL3448 |
| Series (1) |
| GSE4240 |
High Resolution Mapping of DNA Copy Alterations in Human Chromosome 22 Using High Density Tiling Oligonucleotide Arrays |
|
