cell line: VMM39 mutation: Ras treatment: Treated with Diclofenac
Growth protocol
SLM2, VMM39, and DM331 cells were plated and incubated overnight before being treated, in duplicate, with inhibitors or vehicle control in phenol red-free RPMI Medium 1640 without fetal bovine serum for 8 hours at 37°C. Cells were placed on ice and rinsed with cold 1x PBS.
Extracted molecule
total RNA
Extraction protocol
Cells were collected and RNA was isolated using the Qiashredder (Qiagen, Valencia, CA) and RNeasy Mini Kit (Qiagen, Valencia, CA).
Label
biotin
Label protocol
The gene array was performed using Illumina 3’IVT human HT-12 BeadChip arrays by Gene Analysis (Durham, NC).
Hybridization protocol
The gene array was performed using Illumina 3’IVT human HT-12 BeadChip arrays by Gene Analysis (Durham, NC).
Scan protocol
The gene array was performed using Illumina 3’IVT human HT-12 BeadChip arrays by Gene Analysis (Durham, NC).
Description
Cell line VMM39, mutant status Ras, condition Treated with Diclofenac 2
