|
| Status |
Public on Jan 06, 2013 |
| Title |
Muscle from WT, biological rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Soleus muscle, basal state, WT
|
| Organism |
Mus musculus |
| Characteristics |
strain: B6EiC3SnF1/J (mix of B6 and C3H background)
genotype: wild-type
tissue: skeletal muscle
Sex: male
age: 5 months
|
| Treatment protocol |
N/A
|
| Growth protocol |
N/A
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the Qiagen RNeasy Mini Kit.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cDNA was prepared using the NuGEN Technologies Ovation Pico WTA System, WT Ovation Exon Module, and Biotin Module according to the manufacturer's instructions.
|
| |
|
| Hybridization protocol |
Labeled cDNA was hybridized on the Mouse Gene ST array for 18 hours at 45C. Arrays were washed and stained on an Affymetrix Fluidics Station 450 using protocol FS_450_0007.
|
| Scan protocol |
Arrays were scanned on an Affymetrix GeneChip Scanner 7G Plus.
|
| Description |
Muscle_DS15
Gene-level analysis.
|
| Data processing |
Data were analyzed using Expression Console version 1.1 (Affymetrix) using the default RMA algorithm.
|
| |
|
| Submission date |
Jul 06, 2012 |
| Last update date |
Jan 06, 2013 |
| Contact name |
Frank A Middleton |
| E-mail(s) |
[email protected]
|
| Phone |
315-464-7721
|
| Organization name |
SUNY Upstate Medical Univ
|
| Department |
Neuroscience and Physiology
|
| Street address |
505 Irving Avenue
|
| City |
Syracuse |
| State/province |
NY |
| ZIP/Postal code |
13210 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE39159 |
Skeletal muscle gene expression data from Down syndrome mice |
|
