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Sample GSM9527 Query DataSets for GSM9527
Status Public on Oct 29, 2003
Title 12 hr B-chip 1-21-03
Sample type RNA
 
Source name IB3-1 bronchial epithelial cell line
Organism Homo sapiens
Extracted molecule total RNA
 
Description IB3-1 (deltaF508/W1282X) bronchial epithelial cells were cultured in T75 flasks with gentamicin-free LHC-8 medium. After reaching 80% confluence cells were treated with 1 mM PBA for 12 hr. A T75 flask of confluent IB3-1 cells was rinsed twice with ice cold Hank’s buffer then scraped into 3ml of ice cold TRIzol (Gibco BRL) then rinsed with 3 ml ice cold TRIzol and the mRNA was isolated according to the TRIzol protocol. Array images were assessed by eye to confirm scanner alignment and the absence of significant bubbles or scratches. Chip scanning and expression analysis was performed using Microarray Analysis Suite 5.0 software and scaled to an average probe set intensity of 150.
 
Submission date Sep 03, 2003
Last update date Oct 28, 2005
Contact name Jerry M. Wright
E-mail(s) [email protected]
Phone 410-614-5010
Organization name Johns Hopkins Medical Institutions
Department Physiology
Street address 725 N. Wolfe St
City Baltimore
State/province MD
ZIP/Postal code 21205
Country USA
 
Platform ID GPL97
Series (1)
GSE620 Gene expression profile analysis of 4-phenylbutyrate treatment of IB3-1 bronchial epithelial cell line

Data table header descriptions
ID_REF
VALUE chip scaled to 150
ABS_CALL Present/Marginal/Absent

Data table
ID_REF VALUE ABS_CALL
AFFX-BioB-5_at 516.4 P
AFFX-BioB-M_at 712.5 P
AFFX-BioB-3_at 422.2 P
AFFX-BioC-5_at 1255.7 P
AFFX-BioC-3_at 1065.7 P
AFFX-BioDn-5_at 845.5 P
AFFX-BioDn-3_at 5857.0 P
AFFX-CreX-5_at 11640.0 P
AFFX-CreX-3_at 23166.4 P
AFFX-DapX-5_at 5.3 A
AFFX-DapX-M_at 30.9 A
AFFX-DapX-3_at 22.5 A
AFFX-LysX-5_at 8.7 A
AFFX-LysX-M_at 98.2 A
AFFX-LysX-3_at 58.7 A
AFFX-PheX-5_at 4.4 A
AFFX-PheX-M_at 7.7 A
AFFX-PheX-3_at 47.9 A
AFFX-ThrX-5_at 79.8 A
AFFX-ThrX-M_at 83.3 A

Total number of rows: 22645

Table truncated, full table size 393 Kbytes.




Supplementary file Size Download File type/resource
GSM9527.CEL.gz 3.3 Mb (ftp)(http) CEL

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