|
| Status |
Public on Apr 01, 2015 |
| Title |
SAS rep 1 |
| Sample type |
RNA |
| |
|
| Source name |
oral tumor
|
| Organism |
Homo sapiens |
| Characteristics |
gender: male
cell line: SAS
tissue: epithelia
treatment: mock
|
| Growth protocol |
Oral cancer cell lines, such as OC3, SAS, SCC-15, and HSC3, were cultured with or without 10 uM of 5-aza-2’-deoxycytidine (AzC) for 4 days.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using an RNeasy mini kit (Qiagen) according to the manufacturer's instruction.
|
| Label |
biotin
|
| Label protocol |
500ng total RNA was converted into cDNA using biotinylated random nonamers, oligodeoxythymidine 18 primers and Illumina-supplied reagents, according to Illumina manufacturer's instructions.
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
replicate 1
SAS (1)
|
| Data processing |
Data analysis was performed with GeneSpring GX software (Agilent Technologies). Quantile normalization was performed. Differentially expressed gene lists were generated by using Fold Change analysis tool.
|
| |
|
| Submission date |
Jun 20, 2012 |
| Last update date |
Apr 01, 2015 |
| Contact name |
Chia Huei Lee |
| E-mail(s) |
[email protected]
|
| Organization name |
Taiwan National Health Research Institute
|
| Department |
National Institute of Cancer Research
|
| Street address |
35, Keyan Rd.
|
| City |
Zhunan Miaoli County |
| ZIP/Postal code |
114 |
| Country |
Taiwan |
| |
|
| Platform ID |
GPL6883 |
| Series (1) |
| GSE38823 |
Gene expression profiling of oral cancer cell lines with or without AzC treatment |
|
