|
| Status |
Public on Jun 01, 2012 |
| Title |
embryo at HH8_NF_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
cranial neural fold, HH8
|
| Organism |
Gallus gallus |
| Characteristics |
embryo stage: HH8
tissue: cranial neural fold (NF)
|
| Treatment protocol |
Cranial neural folds and neural plates were dissected from HH stage 8 embryos using tungsten needles in ice-cold Hank's balanced salt solution with supplemented with 1 mM MgCl2, 1 mM CaCl2, and 1 mM HEPES at pH 7.5.
|
| Growth protocol |
Fertile chicken eggs were incubated at 38C in a humidified incubator.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The isolated tissues were dissolved in TRIzol (Invitrogen), snap frozen in dry ice/ethanol, and then stored at -80C overnight. Total RNA were isolated according to the manufacturer's protocol.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Chicken Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Data processing |
The data were analyzed with Affymetrix GeneChip Operating System (GCOS) 1.x using Affymetrix MSA5 default analysis settings and global scaling as normalization method.
|
| |
|
| Submission date |
May 31, 2012 |
| Last update date |
Jun 01, 2012 |
| Contact name |
Yukinori Endo |
| E-mail(s) |
[email protected]
|
| Phone |
301-827-0737
|
| Fax |
301-496-1810
|
| Organization name |
NIDCR, NIH
|
| Department |
Laboratory of Cell and Developmental Biology
|
| Lab |
Cell Biology Section
|
| Street address |
30 Convent Drive, MSC 4370, Room 402
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL3213 |
| Series (1) |
| GSE38381 |
Chicken neural crest and neural plate |
|
