|
| Status |
Public on May 24, 2012 |
| Title |
MD10/TetOx2 dox memory rep1 |
| Sample type |
RNA |
| |
|
| Source name |
MD10/TetOx2 exposed to dox, sorted memory cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: U2OS
cell type: MD10/TetOx2
treatment: doxycycline (1 ug/mL, 24 hours)
sorted: memory cells
|
| Treatment protocol |
Cells were either exposed to UV (10uJ/m^2) or doxycycline (1 ug/mL, 24 hours) and allowed to recover 3 days before sortng and RNA extraction
|
| Growth protocol |
Cells were grown in McCoy's 5A + 10% Tet-screened FBS, Pen/strep, and appropriate antibiotics
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the Rneasy kit (Qiagen)
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1ug RNA
|
| |
|
| Hybridization protocol |
cRNA were fragmented and hybridized for overnight at 45C on GeneChip Human Gene 1.0 ST Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
The chip is scanned using a confocal laser scanner
|
| Data processing |
Data were processed using Expression Console (Affymetrix)
|
| |
|
| Submission date |
May 23, 2012 |
| Last update date |
May 24, 2012 |
| Contact name |
Mara Christine Inniss |
| E-mail(s) |
[email protected]
|
| Organization name |
Harvard Medical School
|
| Department |
Systems Biology
|
| Lab |
Silver lab
|
| Street address |
200 Longwood Ave.
|
| City |
Boston |
| ZIP/Postal code |
02215 |
| Country |
USA |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE38189 |
Synthetic circuits for tracking human cell fate |
|
