|
| Status |
Public on May 18, 2012 |
| Title |
KRAS-mutant case 5 (non tumor) |
| Sample type |
RNA |
| |
|
| Source name |
nonneoplastic colon mucosa
|
| Organism |
Homo sapiens |
| Characteristics |
genotype/variation: KRAS-mutant
|
| Treatment protocol |
fresh frozen
|
| Growth protocol |
not applicable
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA of normal and CRC tissue was isolated using the mirVana miRNA Isolation Kit (Ambion/ Applied Biosystems, Darmstadt, Germany) according to the manufacturer’s instruction. To digest DNA, the samples were treated with DNAse of the Turbo DNA-free kit (Ambion). RNA quality was assessed in a 1.5% agarose gel and concentrations of the samples were measured in a NanoDrop 2000 (Thermo Fisher Scientific, Waltham, USA). RNAs were stored at -80°C.
|
| Label |
biotin
|
| Label protocol |
according to the manufacturer`s recommendations
|
| |
|
| Hybridization protocol |
according to the manufacturer`s recommendations
|
| Scan protocol |
according to the manufacturer`s recommendations
|
| Description |
KRAS-mutant colon cancer case # 5 (non tumor)
|
| Data processing |
Data Mining Tool 3.0 (Affymetrix) and GeneSpring software package 7.2 (Silicon Genetics, Redwood City, CA)
|
| |
|
| Submission date |
May 17, 2012 |
| Last update date |
May 18, 2012 |
| Contact name |
Christoph Röcken |
| E-mail(s) |
[email protected]
|
| Phone |
+49(0)4315973401
|
| Fax |
+49(0)4315973462
|
| Organization name |
Christian-Albrechts-University Kiel
|
| Department |
Pathology
|
| Street address |
Arnold-Heller-Str. 3/14
|
| City |
Kiel |
| ZIP/Postal code |
D-24105 |
| Country |
Germany |
| |
|
| Platform ID |
GPL11532 |
| Series (1) |
| GSE38026 |
KRAS-genotype dependent gene expression pattern in colorectal cancer |
|
