|
| Status |
Public on Apr 12, 2012 |
| Title |
dNC-PCs differentiated 1 |
| Sample type |
RNA |
| |
|
| Source name |
dNC-PCs differentiated
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: dental neural crest progenitor cells (dNC-PCs)
culture medium: differentiation medium
|
| Treatment protocol |
After subconfluence cells at passage 6 were grown 7 days in the STEMPRO® Osteogenesis Differentiation Kit (Invitrogen).
|
| Growth protocol |
Cell were grown in DMEM medium (PAA, Pasching, Austria) and 10 % serum at 37°C in 5% CO2.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNAs were extracted using the kit NucleoSpin® RNA II kit (Macherey Nagel, Düren, Germany) and quality-controlled using the RNA 6000 Nano LabChip (Agilent Technologies, Santa Clara, CA, USA).
|
| Label |
biotin
|
| Label protocol |
Double-stranded cDNA was prepared from 5 μg of total cellular RNA (cDNA synthesis kit, Affymetrix Santa Clara, CA, USA), in vitro transcribed using an IVT labeling kit, and the cRNA product purified using a GeneChip Sample Cleanup Module (Affymetrix). All procedures according to the Affymetrix protocol.
|
| |
|
| Hybridization protocol |
Hybridization to Affymetrix oligonucleotide microarray Human Gene 1.1 ST array was carried out overnight with 1biotin-labeled cRNA in a hybridization oven 640.
|
| Scan protocol |
GeneTitan scanner (Affymetrix)
|
| Description |
SAMPLE 7
Gene expression of dNC-PCs at day 7 of differentiation
|
| Data processing |
Affymetrix GeneChip Operating Software (GCOS) 1.4 and the Expression Console 1.1. Software. For the creation of the summarized log-signal the robust multi-chip analysis (RMA) algorithm was used.
|
| |
|
| Submission date |
Apr 11, 2012 |
| Last update date |
Apr 12, 2012 |
| Contact name |
Christian Morsczeck |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Regensburg
|
| Street address |
Franz-Josef Strauss Allee 11
|
| City |
Regensburg |
| ZIP/Postal code |
93053 |
| Country |
Germany |
| |
|
| Platform ID |
GPL11532 |
| Series (1) |
| GSE37189 |
Gene expression profiles of dental neural crest progenitor cells (dNC-PCs) and stem cells of exfoliated descidious teeth (SHED) |
|
